瑞舒伐他汀对载脂蛋白E基因敲除小鼠动脉粥样硬化的治疗作用及其机制

doi:10.13481/j.1671-587x.20160210

Abstract

Abstract:

Objective: To explore the influence of rosuvastatin in the lipid levels, atherosclerosis plaque and apoptosis in the plaque of atherosclerotic apolipoprotein E(ApoE)-knockout mouse models, and to clarify the mechanism of rosuvastatin in inhibiting atheromatous plaque and apoptosis in the plaque.Methods: Thirty healthy six-week old male mice were randomly divided into high fat diet group(n=10), rosuvastatin group(n=10) and general diet control group(n=10).The mice in first two groups were fed with high fat diet, and the mice in general diet control group were fed with general diet;4 weeks later the mice in three groups were respectively given 0.9% NaCl solution, rosuvastatin(10 mg·kg·d^-1) and 0.9% NaCl solution by gavage for 8 weeks.And then ELISA was used to detect the serum lipid levels, the area of atherosclerotic plaque was measured by HE staining, and the apoptosis in plaques was detected by TUNEL method;the expression of apoptosis-assoicated gene Bax protein was measured by immunohistochemical staining.Results: Compared with high fat diet group, the levels of total cholesterol(TC) and low density lipoprotein cholesterol(LDL-C) of the mice in rosuvastatin group were significantly decreased(P<0.05 or P<0.01), and the 1evel of high density lipoprotein cholesterol(HDL-C) was significantly increased(P<0.01).The aortic atherosclerotic plaque of the mice in high fat diet group was massive with a great quantity of foam cells, cholesterol crystal, necrotic cells and inflammatory cells in the plaque;the aortic atherosclerotic plaque of the mice in rosuvastatin group was smaller with less foam cells, necrotic cells and inflammatory cells; the aortic atherosclerotic plaque area of the mice in rosuvastatin group was significantly smaller than that in high fat diet group(P<0.01);the apoptotic index in the aortic atherosclerotic plaque of the mice in rosuvastatin group was significantly lower than that in high fat diet group(P<0.01).Compared with high fat diet group, the expression of Bax protein in rosuvastatin group was significantly decreased.Conclusion: Rosuvastatin may inhibit the progression of atherosclerotic plaque and the apoptosis in plaques through mitochondrial pathway.

Key words: rosuvastatin, aortic atherosclerosis, apoptosis, Bax

CLC Number:

R972

LYU Wenxuan, LI Wenyao, FAN Sijia, ZHAO Shu, GUO Wuhu, CHEN Lijuan, DENG Yue. Therapeutic effect of rosuvastatin on atherosclerosis of apolipoprotein E(ApoE)-knockout mice and its mechanism[J].Journal of Jilin University Medicine Edition, 2016, 42(02): 240-244.

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