IL-17对破骨细胞中MMP-9表达水平的影响及其意义

doi:10.13481/j.1671-587x.20160309

Abstract

Abstract:

Objective: To explore the effects of interleukin-17 (IL-17) on the expression levels of matrix metalloproteinase-9 (MMP-9) in the osteoclasts, and to provide the references for the study on the effect of IL-17 on the orthodontic force-induced root resorption. Methods: The mouse monocytes/macrophages RAW264.7 were cultured and divided into experiment group and control group; the culture medium in experiment group were added with the gradient concentrations of 0.1, 1.0, 10.0, and 100.0 μg·L^-1 IL-17, and set as 0.1,1.0,10.0, and 100.0 μg·L^-1 IL-17 groups,and the culture medium in control group was IL-17-free.The RAW264.7 cells in various groups were induced to form osteoclasts and identified by TRAP staining on the 5th day after induction. ELISA method was used to detect the expression levels of MMP-9 in the culture medium of RAW264.7 cells in various groups when the osteoclasts were induced for 5 d; RT-PCR method was used to detect the expression levels of MMP-9 mRNA in the culture medium of RAW264.7 cells in various groups when the osteoclasts were induced for 5 d. Results: The TRAP staining results showed TRAP staining positive cells were found, with large volume and large number of nucleus (nucleus≥3) on the 5th day after induction. The ELISA and RT-PCR results showed the expression levels of MMP-9 and MMP-9 mRNA in the culture medium of RAW264.7 cells in 1.0,10.0 and 100.0 μg·L^-1IL-17 groups were increased compared with control group(P<0.05); the expression levels of MMP-9 and MMP-9 mRNA in the culture medium of RAW264.7 cells in 10.0 and 100.0 μg·L^-1 IL-17 groups were increased compared with 1.0 μg·L^-1 IL-17 group(P<0.05); the expression levels of MMP-9 and MMP-9 mRNA in the culture medium of RAW264.7 cells in 100.0 μg·L^-1 IL-17 group were increased compared with 10.0 μg·L^-1 IL-17 group(P<0.05). Conclusion: IL-17 can promot the expressions of MMP-9 in the osteoclasts, and the promoting effect is enhanced with the increasing of IL-17 concentration.

Key words: interleukin-17, matrix metalloproteinase-9, RAW264.7, osteoclast

CLC Number:

R329.28

YAO Jindan, HAN Guanghong, HU Min. Effects of IL-17 on expressions of MMP-9 in osteoclasts and their significances[J].Journal of Jilin University Medicine Edition, 2016, 42(03): 462-466.

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Effects of IL-17 on expressions of MMP-9 in osteoclasts and their significances

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