寻常型银屑病患者血清中IL-17的表达及紫草素对IL-17刺激HaCaT细胞分泌IL-6和IL-23的影响

doi:10.13481/j.1671-587x.20170519

Abstract

Abstract: Objective: To explore the expression level of serum interleukin-17 (IL-17) in the patients with psoriasis vulgaris and the changes of interleukin-6 (IL-6) and interleukin-23 (IL-23) levels secrered by the HaCaT cells after stimulated with IL-17, and to clarify its clinical significance and the intervention effect of shikonin. Methods: Twenty-five normal controls, 29 patients with psoriasis vulgaris and different groups of HaCaT cells (blank control group,IL-17-24 h group,IL-17-36 h group,IL-17-48 h group,shikonin+IL-17 group,CsA+IL-17 group and IL-17 group) were used as the subjects. The levels of serum IL-17 in the patients with psoriasis vulgaris and the levels of IL-6 and IL-23 in supernatant of HaCaT cells in various groups were measured by double antibody sandwich enzyme-linked immunosorbent assay(ELISA) and real-time polymerase chain reaction RT-PCR was used to detect the expression levels of IL-6 and IL-23 p19 mRNA in HaCaT cells in various groups.At the same time,Cell Counting Kit-8 (CCK-8) method was used to detect the viabilities of HaCaT cells in various groups. Results: The level of serum IL-17 of the patients in psoriasis vulgaris group was increased compared with nomral control group, especially in the severe skin lesion group(P<0.05). The levels of IL-6 and IL-23 in HaCaT cells and supernatant and their mRNA expression levels in IL-17-24 h,IL-17-36 h and IL-17-48 h groups were significantly higher than those in blank control group (P<0.01). The expression levels of IL-6 and IL-23 in HaCaT cells and supernatant and their mRNA expression levels in shikonin+IL-17 and CsA+IL-17 groups were lower than those in IL-17 groups (P<0.05). No statistical differences were found in the cell viabilities between each drug treatment group and blank control group (P>0.05). Conclusion: The expression level of IL-17 in the patients with psoriasis vulgaris is significantly increased,especially in the patients with severe psoriasis vulgaris.IL-17 can promote the secretion of IL-6 and IL-23 in HaCaT cells in a time-dependent manner. Shikonin can inhibit the proinflammatory effect of IL-17.

Key words: psoriasis, HaCaT cell, interleukin-17, interleukin-6, interleukin-23, shikonin

CLC Number:

R758.63

HANG Min, REN Hongwei, QU Huiming, GENG Long. Clinical significance of IL-17 expression in serum of patients with psoriasis vulgaris and effects of shikonin on IL-6 and IL-23 production in IL-17-stimulated HaCaT cells[J].Journal of Jilin University Medicine Edition, 2017, 43(05): 958-962.

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Clinical significance of IL-17 expression in serum of patients with psoriasis vulgaris and effects of shikonin on IL-6 and IL-23 production in IL-17-stimulated HaCaT cells

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