毛樱桃总黄酮对小鼠皮肤增生性瘢痕的抑制作用及其机制

doi:10.13481/j.1671-587X.20210203

Abstract

Abstract: Objective

To investigate the effect of prunus tomentosa thunb total flavone （PTTTF） on the formation of skin hypertrophic scar （HS） in the mice， and to elucidate its possible mechanism.

Methods

A total of 96 male ICR mice were randomly divided into blank control group， model control group （given same amount of blank matrix as the plastics）， low， middle and high doses of prunus tomentosa thunb plastics （PTTP） groups （given 0.4， 0.8， and 1.6 g·L^－1）PTTP and positive control （DXM） group； there were 16 mice in each group. Except for blank control group， the mice in other groups were used to set up two full-thickness skin defect models with diameter of 10 mm made on both sides of the back by using a skin punch with the spine as the midline. The drug was given once a day and smeared with 0.1 mL each time. The wound healing was observed every day and the wound healing rate was calculated. Four mice in each group were killed on the 10th day， 20th day， 30th day and 40th day， respectively， and the full-thickness skin tissue of the wound was taken.The pathomorphology of HS skin tissue was observed by HE staining. Western blotting method was used to detect the protein expression levels of cysteinyl aspartats specific proteinase-3（Cleaved caspase-3）， B cell lymphoma-2（Bcl-2） and Bcl-2 associated X protein（Bax） in skin tissue of the mice in various groups.

Results

Compared with model control group， on the 40th day， the healing rates of the mice in low， middle and high doses of PTTP groups were 98.94%， 99.14%， and 99.04%， respectively； there were significant differences （P<0.05）. The results of HE staining showed that compared with model control group， the wound in different doses of PTTP groups healed faster and gradually recovered to the normal skin structure， with the formation of skin appendages such as sebaceous glands， hair follicles， sweat glands and so on. The results of Western blotting method showed that compared with model control group， the expression levels of Cleaved caspase-3 and Bax proteins in different doses of PTTP groups were increased significantly （P<0.05 or P<0.01）， while the expression levels of Bcl-2 protein were decreased significantly （P<0.05 or P<0.01）.

Conclusion

PTTTF can promote wound healing and has a certain inhibitory effect on the formation of HS in the mice； and its mechanism may be related to inducing apoptosis.

Key words: prunus tomentosa thunb total flavones, wound healing, hypertrophic scar, apoptosis

CLC Number:

R285.5

Tianqi ZOU,Lin BAI,Zhengxu ZHANG,He LI,Chunmei WANG,Jinghui SUN,Jianguang CHEN,Xi CHEN,Chengyi ZHANG. Inhibitory effect of prunus tomentosa thunb total flavonoids on skin hypertrophic scar in mice and its mechanism[J].Journal of Jilin University(Medicine Edition), 2021, 47(2): 265-274.

Figures/Tables 8

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References 21

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Group	Dry weight of cotton ball granuloma (m/mg)	Inhibitory rate of inflammation (η/%)
Blank	38.88±6.67	0
DXM	24.57±6.88^*	36.80
PTTP
Low dose	28.06±8.54^*	27.83
Middle dose	27.08±6.47^*	30.35
High dose	25.16±3.55^*	35.29

Group	Healing rate of wound
Group	(t/d) 4	7	10	13
Model control	30.690±0.074	54.700±0.105	82.130±0.063	88.580±0.014
DXM	41.660±0.050^**	60.300±0.105	66.650±0.044^*	70.020±0.042^**
PTTP
Low dose	37.930±0.043^*	62.270±0.066	87.020±0.039^*	90.330±0.019^*
Middle dose	42.090±0.068^*	72.660±0.023^*	88.930±0.034^*	92.780±0.016^*
High dose	39.100±0.046^*	64.540±0.150	88.220±0.040	92.550±0.011^**
Group	Healing rate of wound
Group	(t/d) 16	19	22	25
Model control	90.300±0.023	92.690±0.009	93.900±0.007	95.000±0.007
DXM	81.730±0.001^*	87.030±0.007^*	88.650±0.010^*	90.170±0.007^*
PTTP
Low dose	92.910±0.016^*	94.650±0.014	95.730±0.014	96.540±0.009^*
Middle dose	94.180±0.015^*	95.560±0.009^**	96.650±0.007^*	97.080±0.008^*
High dose	93.470±0.011	95.100±0.013^*	95.940±0.012^*	96.780±0.012
Group	Healing rate of wound
Group	(t/d) 28	31	34	37	40
Model control	95.850±0.006	96.510±0.004	96.870±0.004	97.420±0.006	97.930±0.004
DXM	91.180±0.009^*	91.960±0.009^*	93.410±0.003^**	94.380±0.007^*	95.580±0.004^*
PTTP
Low dose	97.000±0.007^*	97.570±0.006	97.950±0.005^*	98.550±0.003^*	98.940±0.002^*
Middle dose	97.500±0.005^*	98.040±0.002^**	98.560±0.002^**	98.900±0.001^*	99.140±0.002^*
High dose	97.230±0.009	97.960±0.004^**	98.310±0.002^*	98.730±0.002^**	99.040±0.002^*

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Inhibitory effect of prunus tomentosa thunb total flavonoids on skin hypertrophic scar in mice and its mechanism

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