MCM10沉默对乳腺癌MDA-MB-231细胞的增殖抑制作用及其机制

doi:10.13481/j.1671-587X.20210315

Abstract

Abstract: Objective

To investigate the inhibitory effect of mini-chromosome maintenance protein 10（MCM10） silencing on the proliferation of breast cancer cells，and to elucidate its mechanism.

Methods

The human triple negative breast cancer （TMBC）MDA-MB-231 cells were divided into control group，MCM10 interference group 1 （shMCM10-1 group）and MCM10 interference group 2（shMCM10-2 group）. The lentivirus interference vectors of MCM10-1 group and MCM10-2 were constructed and infected into the MDA-MB231 cells in shMCM10-1 group and shMCM10-2 group，respectively. The MDA-MB-231 cells in control group were infected with the negative control virus. The expression levels of MCM10 in the MDA-MB-231 cells in various groups were measured by Real-time fluorescence quantitative PCR（RT-qPCR） and Western blotting methods； the proliferation of the MDA-MB-231 cells in various groups was monitored by Cellomics Count assay；the apoptotic rates of the MDA-MB-231 cells in various groups were detected by Flow cytometry， and the activities of Caspase3/7 in the MDA-MB-231 cells in various groups were measured by Caspase 3/7 activity detection kit.

Results

The expression levels of MCM10 mRNA in the MDA-MB-231 cells in shMCM10-1 group and shMCM10-2 group were significantly lower than that in control group （P<0.01）， and the expression of MCM10 protein was not found.Compared with control group， the relative proliferation multiples of the MDA-MB-231 cells in shMCM10-1 group and shMCM10-2 group were significantly decreased （P<0.01），and the apoptotic rates of the MDA-MB-231 cells in shMCM10-1 group and shMCM10-2 group were significantly increased （P<0.01），and the activities of Caspase 3/7 in the MDA-MB-231 cells in shMCM10-1 group and shMCM10-2 group were significantly increased （P<0.01）.

Conclusion

MCM10 gene silencing can inhibit the proliferation of the MDA-MB-231 cells， and promote the apoptosis of the MDA-MB-231 cells and increase the activities of Caspase3/7 in the MDA-MB-231 cells.

Key words: mini-chromosome maintenance protein 10, lentivirus vector, breast neoplasm, MDA-MB-231 cells, apoptosis

CLC Number:

R730.2

Pei GONG, Jingran LIU, Shimin ZHAO, Yuzhen WANG, Jiming XIE. Inhibitory effect of MCM10 silencing on proliferation of breast cancer MDA-MB-231 cells and its mechanism[J].Journal of Jilin University(Medicine Edition), 2021, 47(3): 652-659.

Figures/Tables 8

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References

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Inhibitory effect of MCM10 silencing on proliferation of breast cancer MDA-MB-231 cells and its mechanism

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