Journal of Jilin University(Medicine Edition) ›› 2021, Vol. 47 ›› Issue (4): 874-881.doi: 10.13481/j.1671-587X.20210408
• Research in basic medicine • Previous Articles Next Articles
Yingying SHI1,Shitong CHEN2,Bo HU2,Duo SHEN2,Kuang ZHU2,Siwei YE2,Jinmei FENG3()
Received:
2020-12-12
Online:
2021-07-28
Published:
2021-07-22
Contact:
Jinmei FENG
E-mail:fengjm@jhun.edu.cn
CLC Number:
Yingying SHI,Shitong CHEN,Bo HU,Duo SHEN,Kuang ZHU,Siwei YE,Jinmei FENG. Induction of 3C protein of Coxsackievirus A16 in apoptosis of human rhabdomyosarcoma cells and its mechanism[J].Journal of Jilin University(Medicine Edition), 2021, 47(4): 874-881.
Tab. 1
Primer sequences of PCR"
Gene | Primer sequence(5'-3') |
---|---|
Bak | Forward AGGGCTTAGGACTTGGTTTG |
Reverse GGGATTCCTAGTGGTGTTGATAG | |
Bad | Forward GGAGGATGAGTGACGAGTTTG |
Reverse GGAGCTTTGCCGCATCT | |
PUMA | Forward GTGACCACTGGCATTCATTTG |
Reverse TCCTCCCTCTTCCGAGATTT | |
β-actin | Forward CACGATGGAGGGGCCGGACTCATC |
Reverse TAAAGACCTCTATGCCAACACAGT |
Tab.4
Expression levels of PARP,cleaved-PARP and cleaved-caspase-3 proteins in RD cells in various groups"
Group | PARP | Cleaved- PARP | Cleaved- caspase-3 |
---|---|---|---|
Control pCMV-HA-3C | 0.88±0.01 | 0.71±0.01 | 0.55±0.01 |
0.1 μg | 0.86±0.01 | 0.75±0.01* | 0.60±0.01* |
0.2 μg | 0.82±0.07 | 0.77±0.01* | 0.68±0.01* |
0.4 μg | 0.70±0.02** | 0.99±0.03** | 0.88±0.03** |
Tab. 5
Expression levels of AKT signaling pathway proteins in RD cells in various groups in AKT activator SC79 treatment experiment (n=3,x±s)"
Group | Cleaved-PARP | Cleaved-caspase-3 | AKT | p-AKT |
---|---|---|---|---|
Control | 0.82±0.01 | 0.81±0.01 | 1.02±0.02 | 0.98±0.01 |
0.2 μg pCMV-HA-3C | 1.07±0.01* | 1.16±0.02* | 1.10±0.01* | 0.82±0.01** |
SC79 treatment | 0.84±0.01ΔΔ | 0.97±0.04Δ | 0.94±0.01ΔΔ | 1.10±0.01ΔΔ |
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