Journal of Jilin University(Medicine Edition) ›› 2022, Vol. 48 ›› Issue (2): 291-298.doi: 10.13481/j.1671-587X.20220204

• Research in basic medicine • Previous Articles     Next Articles

Regulatory effect of acupuncture intervention on “drift” and abnormal reorganization of visual cortical orientation columns in rat model of monocular visual deprivation and its mechanism of brain function

Yujuan YE,Juanjuan ZHANG,Chongbing MA,Xingke YAN()   

  1. Acumox and Tuina College,Gansu University of Traditional ChineseMedicine,lanzhou 730000,China
  • Received:2021-06-16 Online:2022-03-28 Published:2022-05-10
  • Contact: Xingke YAN E-mail:yanxingke@126.com

Abstract: Objective

To explore the effect of acupuncture intervention on the “drift” and channel reorganization of visual cortex azimuth column after monocular visual deprivation of the rats, and to preliminarily clarify mechanism.

Methods

A total of 70 healthy SD rats were randomly divided into blank control group (n=10) and right eye suture group (n=60).The rats in right eye suture group were used to establish the visual deprivation models by right eye suture.Then the successful model rats were randomly divided into model group, levodopa methyl ester (LDME) group and early(3 d after model replication), middle(12 d after model replication) and late(21 d after model replication) acupuncture groups(n=10). After the corresponding intervention, the concentrations of oxyhemoglobin(Oxy-Hb), deoxygenated hemoglobin(Deoxy-Hb) and total hemoglobin(Total-Hb) of the rats in various groups were detected by functional near infrared spectroscope (fNIRs) technique.

Results

Compared with blank control group, the concentrations of Oxy-Hb in bilateral visual cortex of 2-5 and 4-4 channels of the rats in model group were significantly decreased (P<0.05); compared with model group,the concentrations of Oxy-Hb and Total-Hb in 2-5 and 4-4 channels in early, middle and late acupuncture groups were significantly increased (P<0.05);the concentrations of Deoxy-Hb were decreased; there were no significant differences in the concentrations of Oxy-Hb, Deoxy-Hb and Total-Hb between the left and right sides of 2-5 and 4-4 channels in blank control group (P>0.05), but the concentration of Oxy-Hb in the right side of the rats in model group was higher than that in the left side (P<0.01). In LDME group, the concentrations of Oxy-Hb and Deoxy-Hb in the right side was higher than that in the left side (P<0.05), but there was no difference in the concentration of Total-Hb (P>0.05). In early acupuncture group, the Oxy-Hb concentration of rats had no difference between the left and right sides (P>0.05), while the concentrations of Deoxy-Hb and Total-Hb in the right side were higher than those in the left side (P<0.05). In middle and late acupuncture groups, the concentrations of Oxy-Hb, Deoxy-Hb and Total-Hb of rats in the right side were higher than those in the left side (P<0.05).

Conclusion

After monocular (right) visual deprivation, the sensitivity and selectivity of orientation information perception of neurons in the ipsilateral visual cortex are significantly inhibited, and the function of orientation column in the ipsilateral visual cortex is abnormal. Early acupuncture can effectively regulate the “drift” and abnormal reorganization of visual cortex orientation column after visual deprivation.

Key words: Amblyopia, Acupuncture, Monocular deprivation, Visual cortex azimuth column, Functional near infrared spectroscopy, Brain function

CLC Number: 

  • R246.9