Journal of Jilin University(Medicine Edition) ›› 2023, Vol. 49 ›› Issue (1): 22-30.doi: 10.13481/j.1671-587X.20230104
• Research in basic medicine • Previous Articles Next Articles
Yaping ZHANG,Haijuan SUI,Enzhi YAN()
Received:
2022-04-21
Online:
2023-01-28
Published:
2023-02-03
Contact:
Enzhi YAN
E-mail:geoge0416@163.com
CLC Number:
Yaping ZHANG,Haijuan SUI,Enzhi YAN. Protective effect of rosmarinic acid on Aβ1-42-induced astrocyte injury by activating Nrf2/HO-1 pathway[J].Journal of Jilin University(Medicine Edition), 2023, 49(1): 22-30.
Tab. 3
Levels of IL-1β, TNF-α, and NO in cell culture fluid in various groups"
Group | IL-1β[ρB/(ng?L-1)] | TNF-α[ρB/(ng?L-1)] | NO[cB/(μmol?L-1)] |
---|---|---|---|
Control | 10.71±0.13 | 2.75±0.36 | 6.86±0.24 |
Aβ1-42 | 126.09±0.75* | 16.35±0.56* | 17.84±0.39* |
RA(20 μmol?L-1)+Aβ1-42 | 94.73±2.44△ | 13.65±0.32△ | 16.04±0.53△ |
RA(50 μmol?L-1)+Aβ1-42 | 78.55±1.18△ | 9.34±0.36△ | 12.35±0.51△ |
RA(100μmol?L-1)+Aβ1-42 | 64.05±1.24△ | 7.17±0.31△ | 8.81±0.36△ |
ML385 | 11.98±0.46 | 3.51±0.30 | 7.79±0.82 |
ML385+RA+Aβ1-42 | 97.35±1.67# | 13.51±0.88# | 16.35±0.83# |
Tab. 4
Expression levels of GFAP, Nrf2, and HO-1 proteins in cells in various groups"
Group | GFAP | Nuc-Nrf2 | Cyt-Nrf2 | HO-1 |
---|---|---|---|---|
Control | 0.21±0.01 | 0.77±0.02 | 0.92±0.01 | 0.54±0.03 |
Aβ1-42 | 0.84±0.03* | 0.45±0.02* | 0.72±0.01* | 0.30±0.03* |
RA+Aβ1-42 | 0.50±0.02△ | 0.89±0.02△ | 0.95±0.01△ | 0.66±0.02△ |
ML385 | 0.24±0.01 | 0.74±0.02 | 0.91±0.01 | 0.52±0.02 |
ML385+RA+Aβ1-42 | 0.75±0.01# | 0.61±0.01# | 0.85±0.01# | 0.45±0.01# |
1 | TIWARI S, ATLURI V, KAUSHIK A, et al. Alzheimer’s disease: pathogenesis, diagnostics, and therapeutics[J]. Int J Nanomedicine, 2019, 14: 5541-5554. |
2 |
ZHU M Q, WANG X Z, SUN L, et al. Can inflammation be resolved in Alzheimer’s disease? [J]. Ther Adv Neurol Disord, 2018, 11.DOI:10.1177/1756286418791107 .
doi: 10.1177/1756286418791107 |
3 | VAISERMAN A, KOLIADA A, LUSHCHAK O. Neuroinflammation in pathogenesis of Alzheimer’s disease: phytochemicals as potential therapeutics[J]. Mech Ageing Dev, 2020, 189: 111259. |
4 | THINGORE C, KSHIRSAGAR V, JUVEKAR A. Amelioration of oxidative stress and neuroinflammation in lipopolysaccharide-induced memory impairment using Rosmarinic acid in mice[J]. Metab Brain Dis, 2021, 36(2): 299-313. |
5 | MIR R H, SHAH A J, MOHI-UD-DIN R, et al. Natural anti-inflammatory compounds as drug candidates in Alzheimer’s disease[J].Curr Med Chem, 2021, 28(23): 4799-4825. |
6 | MIRZA F J, AMBER S, SUMERA, et al. Rosmarinic acid and ursolic acid alleviate deficits in cognition, synaptic regulation and adult hippocampal neurogenesis in an Aβ1-42-induced mouse model of Alzheimer’s disease[J]. Phytomedicine, 2021, 83: 153490. |
7 | QU Z, SUN J C, ZHANG W N, et al. Transcription factor NRF2 as a promising therapeutic target for Alzheimer’s disease[J]. Free Radic Biol Med, 2020, 159: 87-102. |
8 | LUAN H Y, KAN Z C, XU Y, et al. Rosmarinic acid protects against experimental diabetes with cerebral ischemia: relation to inflammation response[J]. J Neuroinflammation, 2013, 10: 28. |
9 | MA Z J, LU Y B, YANG F G, et al. Rosmarinic acid exerts a neuroprotective effect on spinal cord injury by suppressing oxidative stress and inflammation via modulating the Nrf2/HO-1 and TLR4/NF-κB pathways[J]. Toxicol Appl Pharmacol, 2020, 397: 115014. |
10 | 王 卓, 周 瑾, 曾泌梅, 等. 阿魏酸钠对氧糖剥夺所致星形胶质细胞损伤的保护作用及机制研究[J]. 中药药理与临床, 2017, 33(5): 26-30. |
11 | SADICK J S, LIDDELOW S A. Don’t forget astrocytes when targeting Alzheimer’s disease[J]. Br J Pharmacol, 2019, 176(18): 3585-3598. |
12 | VALORI C F, GUIDOTTI G, BRAMBILLA L,et al. Astrocytes: emerging therapeutic targets in neurological disorders[J]. Trends Mol Med, 2019, 25(9): 750-759. |
13 | SCHIWECK J, EICKHOLT B J, MURK K. Important shapeshifter: mechanisms allowing astrocytes to respond to the changing nervous system during development, injury and disease[J]. Front Cell Neurosci, 2018, 12: 261. |
14 | COLOMBO E, BASSANI C, DE ANGELIS A, et al. Siponimod (BAF312) activates Nrf2 while hampering NF-κB in human astrocytes, and protects from astrocyte-induced neurodegeneration[J]. Front Immunol, 2020, 11: 635. |
15 | ONO K, LI L, TAKAMURA Y, et al. Phenolic compounds prevent amyloid β-protein oligomerization and synaptic dysfunction by site-specific binding[J]. J Biol Chem, 2012, 287(18): 14631-14643. |
16 | REN P, CHEN J W, LI B X, et al. Nrf2 ablation promotes Alzheimer’s disease-like pathology in APP/PS1 transgenic mice: the role of neuroinflammation and oxidative stress[J]. Oxid Med Cell Longev, 2020, 2020: 3050971. |
17 | BAHN G, PARK J S, YUN U J, et al. NRF2/ARE pathway negatively regulates BACE1 expression and ameliorates cognitive deficits in mouse Alzheimer’s models[J]. Proc Natl Acad Sci U S A, 2019, 116(25): 12516-12523. |
18 | YAMAMOTO S, KAYAMA T, NOGUCHI-SHINOHARA M, et al. Rosmarinic acid suppresses tau phosphorylation and cognitive decline by downregulating the JNK signaling pathway[J]. NPJ Sci Food, 2021, 5(1): 1. |
19 | WEI Y C, CHEN J X, HU Y H, et al. Rosmarinic acid mitigates lipopolysaccharide-induced neuroinflammatory responses through the inhibition of TLR4 and CD14 expression and NF-κB and NLRP3 inflammasome activation[J]. Inflammation, 2018, 41(2): 732-740. |
20 | SEO J Y, PYO E, AN J P, et al. Andrographolide activates Keap1/Nrf2/ARE/HO-1 pathway in HT22 cells and suppresses microglial activation by Aβ42 through Nrf2-related inflammatory response[J]. Mediators Inflamm, 2017, 2017: 5906189. |
21 | TOM S, RANE A, KATEWA A S, et al. Gedunin inhibits oligomeric Aβ1-42-induced microglia activation via modulation of Nrf2-NF-κB signaling[J].Mol Neurobiol, 2019, 56(11): 7851-7862. |
22 | OKSANEN M, HYÖTYLÄINEN I, TRONTTI K, et al. NF-E2-related factor 2 activation boosts antioxidant defenses and ameliorates inflammatory and amyloid properties in human Presenilin-1 mutated Alzheimer’s disease astrocytes[J]. Glia, 2020, 68(3): 589-599. |
23 | CUADRADO A, ROJO A I, WELLS G, et al. Therapeutic targeting of the NRF2 and KEAP1 partnership in chronic diseases[J]. Nat Rev Drug Discov, 2019, 18(4): 295-317. |
[1] | Mingliu GU,Fengyuan LIN,Xuefeng WU,Jianing ZHOU,Xuechun LU,Peige DU,Liping AN. Improvement effect of Phellinus igniarius acidic polysaccharide on liver fibrosis induced by bile duct ligation in mice and its mechanism [J]. Journal of Jilin University(Medicine Edition), 2022, 48(5): 1167-1174. |
[2] | Feng ZHAO,Shaoqing FAN,Xiaoyan CHENG,Xiaona LI,Changsheng LI,Haojie MA. Effect of intrathecal injection of transient receptor potential cation channel subfamily A member 1 shRNA on neuropathic pain in mice with partial sciatic nerve ligation and its mechanism [J]. Journal of Jilin University(Medicine Edition), 2021, 47(6): 1485-1494. |
[3] | Zehua GONG,Junjie LIU,Jiwei XU,Jianmin LI. Effects of ceftriaxone on activation of astrocytes and inflammation reaction in rats with subarachnoid hemorrhage [J]. Journal of Jilin University(Medicine Edition), 2021, 47(6): 1462-1468. |
[4] | Dimi ZHOU,Lu GAN,Lin CHEN,Chengfang ZHOU,Liang ZENG. Neuroprotective effect of Withaferin A on ischemic stroke rats and its mechanism [J]. Journal of Jilin University(Medicine Edition), 2021, 47(4): 919-925. |
[5] | Qi LIU,Xianchen ZHANG,Xiangyu LI,Yumiao LIN,Yanqi YANG,Enzhi YAN. Inhibitory effect of lithium chloride on Aβ1-42-induced astrocyte glutamate release and its protective effect on hippocampal neuronal injury [J]. Journal of Jilin University(Medicine Edition), 2021, 47(1): 35-43. |
[6] | PEI Dan, LIU Xue. Effect of inhibition of PDGFRα activation on glial cell proliferation and scar formation after brain injury in mice [J]. Journal of Jilin University(Medicine Edition), 2020, 46(05): 1023-1028. |
[7] | ZHANG Qian, FENG Qingxia, ZHOU Zhengyi, LI Ruiqi, FANG Lei, YANG Qiunan, SHENG Yu, LU Xuechun, DU Peige, AN Liping. Improvement effect of eleutheroside B on learning and memory abilities of fatigue mice and its mechanism of activating Keap1/Nrf2/ARE signaling pathway [J]. Journal of Jilin University(Medicine Edition), 2020, 46(04): 771-778. |
[8] | JIANG Ren, FENG Zhiying, LI Ping, LI Hong, LI Shuangyue. Effect of sciatic nervepulsed radiofrequency glial activation levels in spinal dorsal horn in chronic constriction injury rat models and its analgesia effect [J]. Journal of Jilin University(Medicine Edition), 2019, 45(01): 45-50. |
[9] | LIU Linna, CHEN Jieyu, LYU Fangyi, HUA Kouzhen, CAI Damin, YIN Guoli, WANG Junjuan. Effect of fingolimod on proliferation and migration of neural stem cells and astrocytes and its protective mechanism against spinal cord injury in mice [J]. Journal of Jilin University(Medicine Edition), 2018, 44(06): 1218-1222. |
[10] | HUANG Kaiwen, LI Arong, DENG Zhijun, LIU Ruoxuan, LI Changqing, ZHONG Shishun, GUO Jiewen, ZHANG Jie, CHEN Bin. Improvement effect of saponin of litchi seed on oxidative stress of pancreas tissue in impaired glucose regulation rats and its mechanism [J]. Journal of Jilin University Medicine Edition, 2018, 44(04): 746-752. |
[11] | LI Hong-xia,LIU Bo-feng,YAO Zhen-wei,SHAO Yong. Expressinons of heme oxygenase-1 in liver tissue of pregnant rats with intrahepatic cholestasis of pregnancy and brain tissue of fetal rats and significance [J]. Journal of Jilin University Medicine Edition, 2013, 39(4): 726-729. |
[12] | WANG Xiao-song,CHENG Xiu-mei,CAO Huan-min,YAO Xiao-guang,LIU Bin. Expression of HO/CO in kidney tissue of diabetic rats and its significance [J]. J4, 2011, 37(3): 479-482. |
[13] | SHEN Mo-han,WANG Quan-cai,LIU Jia-wei,WEI An-hui,ZHU Jie,YAN Wei-qun. Large scale fermentation and purification technologyof recombinant human β-amyloid peptide 1-42 [J]. J4, 2009, 35(1): 34-38. |
[14] | MA Li-na, YU Hong-mei,ZHAO Wen-jing, LUO Tao, CHEN Sen, NOMOTO Hiroshi, FURUKAWA Shoei. Construction and identification of astrocyte linesexpressing NGF, BDNF, and NT3 steadily [J]. J4, 2005, 31(2): 227-230. |
[15] | MA Li-na, ZHAO Wen-jing, LUO Tao, NOMOTO Hiroshi,FURUKAWA Shoei. Establishment of culture system of conditionalizationfor astrocytes of cerebral cortex in vitro [J]. J4, 2004, 30(5): 727-729. |
|