赖氨藤黄酸对乳腺癌MCF-7细胞增殖和凋亡的影响及其作用机制

doi:10.13481/j.1671-587x.20150308

Abstract

Abstract:

Objective To investigate the effect of gambogic acid lysinate (GAL) on the proliferation and apoptosis of human breast cancer MCF-7 cells and its mechanism, and to provide theoretical foundation for clinical trial and use of GAL against breast cancer. Methods The human breast cancer MCF-7 cells in logarithm growth phase were selected and randomly divided into blank control group, different doses (0.25, 0.50, 1.00, 2.00, 4.00, 8.00 μmol·L^-1) of GAL groups, and different doses(0.25, 0.50, 1.00, 2.00, 4.00, 8.00 μmol·L^-1) of gambogic acid groups.After the MCF-7 cells were treated with GAL or gambogic acid for 48 h, MTT assay was used to detect the proliferation activities of MCF-7 cells.After the MCF-7 cells in logarithm growth phase were treated with 2 μmol·L^-1 GAL for different time (0, 6, 12, 24, 36, 48, 60 h), MTT assay was also used to detect the proliferation activities of MCF-7 cells in various groups.The flow cytometry method and Hoechst 33258 staining were used to analyze the apoptosis of MCF-7 cells in various groups. The expression levels of apoptosis-associated proteins were detected by Western blotting method. Results After 48 h cell culture, the cell viabilities in GAL groups were lower than those in control group (P< 0.05) and the cell viability was inhibited in a dose-dependent manner (P<0.05).After the MCF-7 cells were treated by 2 μmol·L^-1 GAL for different time, the cell proliferation was inhibited in a time-dependent manner (P<0.05).The flow cytometry method and Hoechst 33258 staining Results showed that the apoptotic rates of MCF-7 cells in GAL groups were higher than those in control group (P<0.05).The expressions levels of SIRT1 in GAL groups were significantly lower than that in control group (P<0.05), while the expression amounts of cleaved caspase-3 were higher than that in control group (P<0.05). Conclusion GAL can induce the apoptosis of breast cancer MCF-7 cells by inhibiting the expression level of SIRT1 and up-regulating the expression amount of cleaved caspase-3.

Key words: gambogic acid lysinate, breast neoplasms, apoptosis, silent information regulator 1

CLC Number:

R737.9

WEI Jie, LI Kaiji, WEI Jingbo, ZHAO Yufang, YAN Feng, LYU Cuiping, ZHEN Yongzhan. Influence of gambogic acid lysinate in proliferation and apoptosis in breast cancer MCF-7 cells and its mechanism[J].Journal of Jilin University Medicine Edition, 2015, 41(03): 476-480.

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Influence of gambogic acid lysinate in proliferation and apoptosis in breast cancer MCF-7 cells and its mechanism

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