Abstract:

Abstract:Objective To observe the distribution of oligodexynucleotide (ODN) MT01 in main organ tissues  of the rats at different time points and to discuss the regularity of the distribution of MT01 preliminarily.Methods 60 male Wistar rats were randomly divided into experimental group(n=30) and control group(n=30).The rats in experimental group was locally injected  with Cy5 labeled MT01 in gingival mucosa,whereas the rats in control group were injected  with MTO1.The  samples of rat lung,liver spleen,kidney,heart,and brain tissues  were collected at 15 min,1 h,4 h,8 h,16 h,1 d,2 d,3 d,4 d,and 5 d after injection, and the distribution of MT01 fluorescence was observed by laser scanning confocal microscope.The ratio of fluorescence positive cells indicated the amount of MT01 that had been taken up by different organs.Results No positive fluorescence cells were observed in control group.Whereas，in experimental group ，the positive fluorescence cells were detected in the tissue samples of lung,liver,spleen and kidney but not in the tissue samples of heart and brain.The positive fluorescence cells  distributed focally in kidney tissue and presented primarily in the cytoplasm of renal tubular epithelial cells.The ratios of positive fluorescence cells changed regularly with time in liver,spleen and kidney tissues and the highest level was detected at 4，3 and 4 d after injection.No distinct regularity of the ratio of positive fluorescence cells was observed in lung tissue.Conclusion MT01 can be taken up by liver，spleen and lung tissue and prima
rily by kidney with regularity in distribution.

Key words: oligodeoxynucleotide, MT01, Cy5-labeled, rats,Wistar