针刺单穴和腧穴配伍对胃溃疡大鼠的干预效应及其机制

doi:10.13481/j.1671-587x.20200604

Abstract

Abstract: Objective

To investigate the intervention effects of single acupoint and acupoint compatibility on the gastric mucosal injury index， the morphology of gastric mucosa and the expression of epidermal growth factor receptor （EGFR ）protein in gastric tissue of the rats with gastric ulcer， and to clarify the effect of single acupoint and acupoint compatibility and the mechanism of acupuncture for treatment of gastric ulcer in the rats.

Methods

A total of 100 SD rats were randomly divided into blank group， model group， Zusanli group， Zhongwan group， and acupoint compatibility group，and there were 20 rats in each group. Except for blank group， the rats in the other groups were subjected to restraint-water immersion stress method to establish the stress gastric ulcer rat models.After the models were successfully established， no intervention was given to the rats in blank group and model group，and the rats in Zhongwan group， Zusanli group，and acupoint compatibility group were respectively treated with Zhongwan， Zusanli， and Zusanli+Zhongwan electroacupuncture treatment. After 10 d， the gastric ulcer of the rats in various groups was observed， the gastric mucosal injury indexes of the rats in various groups were detected， the pathomorphology of gastric tissue of the rats in various groups was observed by HE staining， and the expression levels of EGFR protein in gastric mucosa tissue of the rats in various groups was detected by immunohistochemistry.

Results

The gastric ulcer index of the rats in model group was higher than that in blank group （P<0.05）， and the gastric ulcer indexes of the rats in Zhongwan group， Zusanli group and acupoint compatibility group were lower than that in model group （P<0.05）. The HE staining results showed that the gastric mucosa of the rats in model group was destroyed，and the gastric mucosa of the rats in Zusanli group， Zhongwan group， and acupoint compatibility group had varying degrees of repair and healing，especially in acupoint compatibility group. The expression level of EGFR protein in gastric mucosa tissue of the rats in model group was higher than that in blank group （P<0.05）；compared with Zhongwan group，the expression level of EGFR protein in gastric mucosa tissue of the rats in Zusanli group was increased （P<0.05），and the expression level of EGFR protein in gastric mucosa tissue of the rats in acupoint compatibility group was increased （P<0.05）； compared with Zusanli group， the expression level of EGFR protein in gastric mucosa tissue of the rats in acupoint compatibility group was increased （P<0.05）.

Conclusion

Electroacupuncture has therapeutic effect on the gastric ulcer by promoting the expression of EGFR， and its mechanism may be related to that EGFR can control the proliferation， differentiation and survival of the gastric mucosal cells.

Key words: compatibility of acupoints, gastric ulcer, epidermal growth factor receptor, immunohistochemical method, gastric mucosal injury index

CLC Number:

R245

Lijuan HA,Fuchun WANG,Xiaona LIU. Intervention effects of single acupoint and acupoint compatibility in gastric ulcer rats and their mechanisms[J].Journal of Jilin University(Medicine Edition), 2020, 46(6): 1131-1136.

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Intervention effects of single acupoint and acupoint compatibility in gastric ulcer rats and their mechanisms

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