lncRNA-CCAT2对宫颈癌CaSki细胞凋亡的影响及其机制

doi:10.13481/j.1671-587x.20200622

Abstract

Abstract: Objective

To investigate the effect of long non-coding RNA （lncRNA）-colon cancer-associated transcript 2 （CCAT2） on the apoptosis of cervical cancer CaSki cells and its mechanism.

Methods

The human cervical cancer CaSki cells were divided into control group， over-expression CCAT2 group， sh-CCAT2 group， over-expression NC group and sh-NC group. The over-expression and interference vectors of CCAT2 were constructed and transfected into the cervical cancer CaSki cells in over-expression CCAT2 and sh-CCAT2 groups， respectively. The expression levels of CCAT2 mRNA in cervical cancer CaSki cells in various groups were detected by qRT-PCR method； MTT assay was used to detect the viability of cervical cancer CaSki cells in various groups；flow cytometry was used to detect the apoptotic rates of cervical cancer CaSki cells in various groups； qRT-PCR method was used to detect the expression levels of phosphatase and tensin homolog deleted on chromosome ten （PTEN） mRNA in cervical cancer CaSki cells in various groups；Western blotting method was used to detect the expression levels of PTEN， phosphoinositide 3-kinase （PI3K），phosphorylated PI3K（ p-PI3K）， protein kinase B （AKT），phosphorylated AKT （p-AKT）， B-cell lymphoma-2 （Bcl-2）， and Bcl-2 associated X protein （Bax） proteins in the cervical cancer CaSki cells in various groups.

Results

Compared with control group， the cell proliferation rate and the expression levels of CCAT2 mRNA and Bcl-2， p-PI3K，and p-AKT proteins in the cervical cancer CaSki cells in over-expression CCAT2 group were significantly increased（P<0.05）， while the apoptotic rate and the expression levels of Bax protein， PTEN mRNA and protein were significantly decreased （P<0.05）. Compared with control group， the proliferation rate of cervical cancer CaSki cells and the expression levels of CCAT2 mRNA and Bcl-2， p-PI3K， and p-AKT proteins in the cervical cancer CaSki cells in sh-CCAT2 group were significantly decreased （P<0.05）； the apoptotic rate and the expression levels of Bax protein， PTEN mRNA and protein were significantly increased （P<0.05）. Compared with control group， the cell proliferation rate， apoptotic rate， and expression levels of CCAT2 mRNA and PTEN， Bcl-2， Bax， p-PI3K，and p-AKT proteins in the cervical cancer CaSki cells in over-expression NC group and sh-NC group had no significant differences（P>0.05）.

Conclusion

LncRNA-CCAT2 can inhibit the apoptosis of cervical cancer cells， and its mechanism may be related to the PTEN/PI3K/AKT pathway.

Key words: colon cancer-associated transcript 2, cervical cancer, cell apoptosis, phosphatase and tensin homolog deleted on chromosome ten, phosphoinositide 3-kinase, protein kinase B

CLC Number:

R737.33

Xia WANG,Yujun YAO,Jing TANG,Kaihui LI. Effect of lncRNA-CCAT2 on apoptosis of cervical cancer CaSki cells and its mechanism[J].Journal of Jilin University(Medicine Edition), 2020, 46(6): 1247-1253.

Figures/Tables 6

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References 23

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Effect of lncRNA-CCAT2 on apoptosis of cervical cancer CaSki cells and its mechanism

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