Journal of Jilin University(Medicine Edition) ›› 2022, Vol. 48 ›› Issue (6): 1498-1509.doi: 10.13481/j.1671-587X.20220616
• Research in clinical medicine • Previous Articles Next Articles
Yun LIU,Linqi ZHU,Shihe SHAO()
Received:
2022-02-21
Online:
2022-11-28
Published:
2022-12-07
Contact:
Shihe SHAO
E-mail:shaoshihe2006@163.com
CLC Number:
Yun LIU,Linqi ZHU,Shihe SHAO. Effects of circular RNA hsa_circ_0009735 on epithelial mesenchymal transformation, cell cycle, and autophagy of gastric cancer cells[J].Journal of Jilin University(Medicine Edition), 2022, 48(6): 1498-1509.
Tab.3
Percentages of cells at different cell cycles in various groups"
Group | Percentage of cells | ||
---|---|---|---|
G1 | S | G2 | |
Negative control | 49.470±2.341 | 25.070±0.933 | 23.300±1.612 |
si-hsa_circ_0009735 | 69.120±2.539* | 15.540±0.955** | 11.820±0.290* |
Vector plasmid | 45.610±1.004 | 20.080±0.566 | 28.200±1.273 |
OE-hsa_circ_0009735 | 25.720±0.690△△ | 31.500±1.838△ | 34.200±1.414△ |
Tab. 4
Ratios of LC3Ⅱ/LC3Ⅰ and expression levels of E-Cadherin, Cyclin D1, Vimentin,and N-Cadherin proteins in cells in various groups"
Group | LC3Ⅱ/LC3Ⅰ | E-Cadherin | Cyclin D1 | Vimentin | N-Cadherin |
---|---|---|---|---|---|
Negative control | 0.435±0.086 | 0.951±0.060 | 0.982±0.026 | 0.851±0.018 | 0.967±0.036 |
si-hsa_circ_0009735 | 1.273±0.001** | 1.066±0.073 | 0.368±0.118* | 0.470±0.034** | 0.619±0.016** |
Vector plasmid | 1.254±0.001 | 1.240±0.009 | 0.609±0.040 | 0.587±0.005 | 0.567±0.060 |
OE-hsa_circ_0009735 | 1.007±0.015△△ | 1.025±0.058△ | 0.994±0.078△ | 0.969±0.067△ | 1.052±0.046△ |
1 | BRAY F, FERLAY J, SOERJOMATARAM I, et al. Global cancer statistics 2018: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries[J]. CA Cancer J Clin, 2018, 68(6): 394-424. |
2 | LIU Y, SHAO C, ZHU L Q, et al. High expression of ABL2 suppresses apoptosis in gastric cancer[J]. Dig Dis Sci, 2018, 63(9): 2294-2300. |
3 | SMYTH E C, NILSSON M, GRABSCH H I, et al. Gastric cancer[J]. Lancet, 2020, 396(10251): 635-648. |
4 | WANG F, MENG W B, WANG B Y, et al. Helicobacter pylori-induced gastric inflammation and gastric cancer[J]. Cancer Lett, 2014, 345(2): 196-202. |
5 | ALIPOUR M. Molecular mechanism of helicobacter pylori-induced gastric cancer[J]. J Gastrointest Cancer, 2021, 52(1): 23-30. |
6 | JOHNSTON F M, BECKMAN M. Updates on management of gastric cancer[J]. Curr Oncol Rep, 2019, 21(8): 67. |
7 | WEI L, SUN J J, ZHANG N S, et al. Noncoding RNAs in gastric cancer: implications for drug resistance[J]. Mol Cancer, 2020, 19(1): 62. |
8 | LI R, JIANG J J, SHI H, et al. CircRNA: a rising star in gastric cancer[J]. Cell Mol Life Sci, 2020, 77(9): 1661-1680. |
9 | YIN Y T, LONG J L, HE Q L, et al. Emerging roles of circRNA in formation and progression of cancer[J]. J Cancer, 2019, 10(21): 5015-5021. |
10 | SANGER H L, KLOTZ G, RIESNER D, et al. Viroids are single-stranded covalently closed circular RNA molecules existing as highly base-paired rod-like structures[J]. Proc Natl Acad Sci U S A,1976,73(11): 3852-3856. |
11 | GUARNERIO J, BEZZI M, JEONG J C, et al. Oncogenic role of fusion-circRNAs derived from cancer-associated chromosomal translocations[J]. Cell, 2016, 165(2): 289-302. |
12 | 郑蓓怡,李之印,叶月芳.胃蛋白酶原及胃泌素-17对筛查胃癌及胃上皮内瘤变的价值[J].中国实用内科杂志,2021,41(6):545-549. |
13 | 张 辉, 梁 鸿, 白军伟, 等. 环状RNA circFOXO3在胃癌组织中的表达及其对胃癌细胞增殖和侵袭转移能力的影响[J].中华实验外科杂志,2020,37(3):408-411. |
14 | LIU J H, QIU G L, WANG H J, et al. CircRNA-ABCB10 promotes gastric cancer progression by sponging miR-1915-3p to upregulate RaC1 [J]. Dig Liver Dis, 2022, 54(7): 896-904. |
15 | PENG L, SANG H M, WEI S C, et al. circCUL2 regulates gastric cancer malignant transformation and cisplatin resistance by modulating autophagy activation via miR-142-3p/ROCK2[J]. Mol Cancer, 2020, 19: 156. |
16 | MA L, WANG Z, XIE M,et al. Silencing of circRACGAP1 sensitizes gastric cancer cells to apatinib via modulating autophagy by targeting miR-3657 and ATG7 [J]. Cell Death Dis,2020,11(3):169-187. |
17 | BAJ J, KORONA-GŁOWNIAK I, FORMA A, et al. Mechanisms of the epithelial-mesenchymal transition and tumor microenvironment in helicobacter pylori-induced gastric cancer[J]. Cells, 2020, 9(4): 1055. |
18 | YU Z B, HUANG H, YI X, et al. EMT (Epithelial-mesenchymal transition) in gastric cancer identifies prognostic and immunotherapeutically relevant gene signatures[J]. Asian J Surg, 2021, 44(5): 792-793. |
19 | 赵德贞, 孔 超, 李印虎. circ_0092306靶向miR-411调控胃癌细胞增殖、凋亡及上皮间质转化的机制研究[J]. 河北医药, 2022, 44(3): 330-335. |
20 | ZHOU L H, YANG Y C, ZHANG R Y, et al. CircRNA_0023642 promotes migration and invasion of gastric cancer cells by regulating EMT[J]. Eur Rev Med Pharmacol Sci, 2018, 22(8): 2297-2303. |
21 | WU J M, CHEN Z H, SONG Y H, et al. CircRNA_0005075 suppresses carcinogenesis via regulating miR-431/p53/epithelial-mesenchymal transition axis in gastric cancer[J]. Cell Biochem Funct, 2020, 38(7): 932-942. |
22 | LIU J, DAI X, GUO X, et al. Circ-OXCT1 suppresses gastric cancer EMT and metastasis by attenuating TGF-β pathway through the circ-OXCT1/miR-136/SMAD4 axis[J]. Onco Targets Ther, 2020, 13: 3987-3998. |
23 | MONTALTO F I, DE AMICIS F. Cyclin D1 in cancer: a molecular connection for cell cycle control, adhesion and invasion in tumor and stroma[J]. Cells, 2020, 9(12): 2648. |
24 | 刘 璐, 张天夫, 王晓峰, 等. 多配体蛋白聚糖1对舌鳞状细胞癌CAL27细胞迁移、侵袭和细胞周期的调控作用[J].吉林大学学报(医学版),2021,47(1):59-65. |
25 | 刘岚清, 李瑞林, 周晓敏, 等. CDC14B在小鼠1-细胞期受精卵各细胞周期的动态表达及其定位[J].吉林大学学报(医学版),2020,46(4):675-679. |
26 | KUMARI S, Puneet, PRASAD S B, et al. Cyclin D1 and cyclin E2 are differentially expressed in gastric cancer[J]. Med Oncol, 2016, 33(5): 40. |
27 | IBRAHIM H M, ABDELBARY A M, MOHAMED S Y,et al. Prognostic value of cyclin D1 and CD44 expression in gastric adenocarcinoma[J]. J Gastrointest Cancer, 2019, 50(3): 370-379. |
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