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An Enzyme from a Thermophilic Bacteria and the Identification of the Strain
TENG Lirong, GAO Chaohui,, WANG Jiapeng,, MENG Qingfan,LU Jiahui, ZHANG Yingjiu,
J4. 2006, 44 (05):
829-833.
A thermophilic strain CW2, which can produce a type of starchdegrading enzyme, was isolated from the soil around a hot spring in the Changbai Mountains. The products from starch by the enzyme were determined by thin layer chromatography (TLC) and the results show that the enzyme is a kind of ectoenzyme that could catalyze the
degradation of starch to produce isomaltooligosaccharide including isomaltose, isomaltotriose, and panose as dominant products. The 16S rDNA of the strain was amp
lified by PCR and was sequenced (873bp), and then was compared with those available in the GenBank databases. The result shows that CW2 16S rDNA sequence is most similar to that of Bacillus licheniformis ATCC 14580,Bacillus licheniformis DSM 13 and Bacillus subtilis strain JM4,
there are only 9,9,13 different bases between every pair of sequences, the sequence similarities are 98.97%,98.97%,98.51%, respectively. The phylogenetic trees including CW2 were derived with neighbor joining and analyzed with Bootstraping. The results demonstrate that CW2 strain
is close consanguinity to Bacillus licheniformis and Bacillus sonorensiss,
they belong to the same sister cluster in Bacillus group.
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