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Screening Traditional Chinese Medicines for Inhibitorsof Protein Tyrosine Phosphatase SHP-1
LI Wannan, LI Ying, ZHUANG Yan, LI He, CHEN Yingli, ZHAO Zhizhuang,, FU Xueqi
J4. 2008, 46 (06):
1211-1216.
With pT7 as a vector, ΔSHP-1, a recombinant protein containing the catalytic domain of protein tyrosine phosphatase SHP-1, was highl
y expressed in E.coli cells. The enzyme was further purified to near homogeneity. With the purified recombinant enzyme as a target, aqueous extracts of 157 traditional Chinese herb medicines were analyzed for their abilities to inhibit SHP-1. Two most potent inhibitors, namely, cornel and dandelion, were identified, and their IC50 values and inhibitory types were further analyzed. This study thus established a good system to screen inhibitors of SHP-1 and demonstrated the potential of traditional Chinese medicines in treatment of immunological diseases and diabetes.
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