吉林大学学报(医学版) ›› 2021, Vol. 47 ›› Issue (5): 1244-1249.doi: 10.13481/j.1671-587X.20210523

• 临床研究 • 上一篇    下一篇

Tip60蛋白在子宫内膜腺癌组织中的表达及其临床意义

文爱平1,罗乐2,孟志伟3,金景姬4,周洪贵1(),朱继红5()   

  1. 1.川北医学院附属医院妇产科,四川 南充 637000
    2.医学影像四川省重点实验室,四川 南充 637000
    3.西藏军区总医院妇产科,西藏 拉萨 850007
    4.吉林大学生命科学院 艾滋病国家重点 实验室,吉林 长春 130012
    5.吉林大学第一医院生殖中心,吉林 长春 130021
  • 收稿日期:2021-02-01 出版日期:2021-09-28 发布日期:2021-10-26
  • 通讯作者: 周洪贵,朱继红 E-mail:hongguizhou@163.com;jhzhu@jlu.edu.cn
  • 作者简介:文爱平(1984-),男,四川省仪陇县人,主治医师,医学博士,主要从事妇科肿瘤微创和表观遗传学方面的研究。
  • 基金资助:
    国家自然科学基金项目(31070668);中央财政支持地方高校专项资金项目(2011JTD0030);四川省南充市市校合作课题-应用基础课题研究项目(18SXHZ0251);川北医学院附属医院科研项目(2017138-137)

Expression of Tip60 protein in endometrial adenocarcinoma tissue and its clinical significance

Aiping WEN1,Le LUO2,Zhiwei MENG3,Jingji JIN4,Honggui ZHOU1(),Jihong ZHU5()   

  1. 1.Department of Gynecology and Obstetrics,Affiliated Hospital,North Sichuan Medical College,Sichuan Province,Nanchong 637000,China
    2.Sichuan Key Laboratory of Medical Imaging,Affiliated Hospital,North Sichuan Medical College,Sichuan Province,Nanchong 637000,China
    3.Department of Gynecology and Obstetrics,General Hospital,Tibet Military Region,Lasha 850007,China
    4.School of Life Science,Jilin University,Changchun 130012,China
    5.Department of Reproductive Center,First Hospital,Jilin University,Changchun 130021,China
  • Received:2021-02-01 Online:2021-09-28 Published:2021-10-26
  • Contact: Honggui ZHOU,Jihong ZHU E-mail:hongguizhou@163.com;jhzhu@jlu.edu.cn

摘要: 目的

探讨子宫内膜腺癌组织中Tip60蛋白的表达与临床病理特征及预后之间的关系,初步阐明Tip60蛋白在子宫内膜腺癌发生发展过程中的作用。

方法

选取子宫内膜腺癌患者84例,采用免疫组织化学SP法检测84例子宫内膜腺癌组织和36例正常子宫内膜组织中Tip60蛋白表达水平,分析其与患者临床病理特征之间的关系;采用Kaplan-Meier曲线分析Tip60蛋白的表达与患者预后的关系;采用Western blotting法检测人子宫内膜癌细胞株ISHIKAWA和HEC-1A中Tip60蛋白表达水平。

结果

免疫组织化学检测,子宫内膜腺癌组织中Tip60蛋白阳性表达率为54.76%(46/84),明显低于正常子宫组织中Tip60蛋白阳性表达率[83.33%(30/36)](χ2=8.859,P=0.003)。Tip60蛋白表达与患者年龄无关(χ2=1.073,P=0.300); FIGO分期(临床病理分期)中,Ⅰ期子宫内膜腺癌患者癌组织中Tip60蛋白阳性表达率明显高于Ⅱ期和Ⅲ-Ⅳ期患者(χ2=13.481,P=0.001);高分化子宫内膜腺癌患者癌组织中Tip60蛋白阳性表达率明显高于低分化和中分化癌组织的患者(χ2=8.962,P=0.013)。子宫内膜腺癌患者的中位随访时间为58个月,Kaplan-Meier生存分析及Log-rank检验,Tip60蛋白高表达的患者生存率明显高于低表达患者(χ2=64.391,P=0.002)。高表达雌激素受体(ER)的ISHIKAWA细胞中Tip60蛋白表达水平明显高于低表达ER的HEC-1A细胞(P<0.05)。

结论

Tip60蛋白在子宫内膜腺癌患者癌组织中和高表达ER的ISHIKAWA细胞中呈低表达,提示其参与子宫内膜腺癌的发生发展,检测组织中Tip60蛋白表达水平有助于子宫内膜腺癌的诊断及评估预后。

关键词: Tip60蛋白, 子宫内膜癌, 临床病理特征, 预后, 免疫组织化学

Abstract: Objective

To investigate the relationships between the expression of Tip60 protein in the endometrial adenocarcinoma tissue and the clinicopathological parameters and prognosis of endometrial adenocarcinoma,and to clarify the effect of Tip60 protein in the occurrence and development of endometrial adenocarcinoma.

Methods

A toatal of 84 cases of endometrial adenocarcinoma patients were selected. The expression levels of Tip60 protein in 84 cases of endometrial adenocarcinoma tissue and 36 cases of normal endometrial tissue were selected with immunohistochemical SP method. The relationships between the expression of Tip60 protein and the clinicopathological parameters were analyzed. Kaplan-Meier curve was used to analyze the relationships between the expression of Tip60 protein and prognosis of the patients. Western blotting method was used to detect the expressions of Tip60 in the endometrial carcinoma cell lines ISHIKAWA and HEC-1A.

Results

The immunohistochemistry results showed that the positive expression rate of Tip60 protein in the endometrial adenocarcinoma tissue was 54.76% (46/84), which was significantly lower than that in the normal endometrial tissue (83.33%, 30/36) (χ2=8.859,P=0.003).The expression of Tip60 protein was not associated with age of the patients(χ2=1.073,P=0.300); the positive expression rate of Tip60 protein in endometrial adenocarcinoma tissue of the patients in grade Ⅰ of FIGO stage was significantly high than those of the patients in grade Ⅰ and grade Ⅲ(χ2=13.481,P=0.001);the positive expression rate of Tip60 protein in the endometrial adenocarcinoma patients with high differentiation was higher than that in the patients with low and middle differentiationχ2=8.962,P=0.013). The median follow-up time for 84 patients with endometrial adenocarcinoma was 58 months. The results of Kaplan-Meier survival analysis and Log-rank test showed that the survival rate of the patients with high expression of Tip60 protein was significantly higher than that of the patients with low expression(χ2=64.391, P=0.002). The expression level of Tip60 protein in the ISHIKAWA cells with high expression of estrogen receptor(ER) was significantly higher than that of HEC-1A cells with low expression of ER (P<0.05).

Conclusion

The expressions of Tip60 protein in endometrial adeno carcinoma tissue and Ishikawa cells with high expression of ER are lower which suggests that Tip60 protein is involved in the occurrence and development of endometrial adenocarcinoma. The detection of Tip60 protein expression level in endometrial tissue is helpful for the diagnosis and prognosis of endometrial adenocarcinoma.

Key words: Tip60 protein, endometrial carcinoma, pathological characteristic, prognosis, immunohistochemistry

中图分类号: 

  • R737.33