吉林大学学报(医学版) ›› 2017, Vol. 43 ›› Issue (04): 752-756.doi: 10.13481/j.1671-587x.20170418

• 基础研究 • 上一篇    下一篇

MT01对实验性牙移动大鼠牙周组织中TLR9、TRAF6和IL-6表达水平的影响

杨竹君1,2, 侯旭1, 于文雯1, 闫阔3, 申玉芹4, 孙新华1   

  1. 1. 吉林大学口腔医院正畸科, 吉林 长春 130021;
    2. 吉林省牙发育及颌骨重塑与再生重点实验室, 吉林 长春 130021;
    3. 吉林大学口腔医院儿童口腔科, 吉林长春 130021;
    4. 吉林大学口腔医院牙周病科, 吉林 长春 130021
  • 收稿日期:2016-09-19 出版日期:2017-07-28 发布日期:2017-08-01
  • 通讯作者: 孙新华,教授,博士研究生导师(Tel:0431-88796023,E-mail:xinhuasun8@163.com) E-mail:xinhuasun8@163.com
  • 作者简介:杨竹君(1992-)女,陕西省蓝田县人,在读医学硕士,主要从事牙齿移动生物学方面的研究。
  • 基金资助:
    吉林省科技厅自然科学基金资助课题(20150101173JC);吉林大学白求恩青年基金资助课题(2013208062)

Effects of MT01 on expressions of TLR9,TRAF6 and IL6 in periodontal tissue during experimental tooth movement of rats

YANG Zhujun1,2, HOU Xu1, YU Wenwen1, YAN Kuo3, SHEN Yuqin4, SUN Xinhua1   

  1. 1. Department of Orthodontics, Stomatology Hospital, Jilin University, Changchun 130021, China;
    2. Jilin Provincial Key Laboratory of Tooth Development and Bone Remodel, Changchun 130021, China;
    3. Department of Pediatric Dentistry, Stomatology Hospital, Jilin University, Changchun 130021, China;
    4. Department of Periodontology, Stomatology Hospital, Jilin University, Changchun 130021, China
  • Received:2016-09-19 Online:2017-07-28 Published:2017-08-01

摘要: 目的:探讨Toll样受体9(TLR9)及其下游炎症因子白细胞介素6(IL-6)在实验性大鼠牙移动过程中牙周组织中表达水平的变化,以及MT01对牙周组织TLR9、肿瘤坏死因子受体相关因子6(TRAF6)和IL-6表达水平的影响,阐明其相关机制。方法:30只雄性Wistar大鼠分为无MT01干预组(n=24)和MT01干预组(n=6)。其中,无MT01干预大鼠右侧加力为实验组,左侧不加力为对照组;MT01干预大鼠左侧上颌第一磨牙颊侧黏膜注射MT01+加力为实验组,右侧上颌第一磨牙颊侧黏膜注射PBS+加力作为对照组。0.49 N力近中移动大鼠上颌第一磨牙,无MT01干预大鼠于加力后第3、7、14和21天处死,MT01干预组于加力后第7天处死。分别获取各组大鼠上颌第一磨牙近远中侧牙槽骨,采用实时荧光定量PCR(RT-PCR)法检测张力侧和压力侧牙周组织中TLR9、TRAF6和IL-6mRNA的表达水平。结果:无MT01干预大鼠中,实验组张力侧和压力侧牙周组织中IL-6和TLR9表达水平均明显高于对照组(P<0.05),且加力7d后两者表达水平达高峰;MT01干预大鼠中,加力7d后实验组张力侧及压力侧牙周组织中TLR9表达水平较对照组降低(P<0.01),实验组压力侧牙周组织中TRAF6表达水平较对照组降低(P<0.01)。结论:MT01能够抑制实验性牙移动大鼠牙周组织中TLR9及下游相关因子TRAF6的表达,进而产生抑制牙移动过程中牙周组织炎症反应的作用。

关键词: 特定序列寡核苷酸MT01, Toll样受体9, 牙移动, 肿瘤坏死因子受体相关因子6, 白细胞介素6, 聚合酶链式反应

Abstract: Objective: To investigate the changes of expression levels of TLR9 and IL-6 in the periodontal tissue during the experimental tooth movement of the rats, and the effecst of the MT01 on the expression of TLR9,TRAF6 and IL-6 in periodontal tissue,and to clarify its related mechanisms.Methods: Thirty Wistar rats were randomly divided into MT01 intervention group(n=6) and non-MT01 group(n=24). Force of 0.49 N was applied to move the upper first molars mesially. The rats in Non-MT01 intervention group were sacrificed on the days 3,7,14 and 21, and the rats in MT01 intervention group were all sacrificed on the day 7. RT-qPCR was used to detect the expression levels of TLR9,TRAF6 and IL-6 mRNA in maxillary first molar alveolar bone in each group.Results: The expression levels of IL-6 and TLR9 mRNA in loaded side were higher than those in control side(P<0.05), and reached the maximum levels on the day 7(P<0.01); with the interpose of MT01, the expression levels of TLR9 and TRAF6 mRNA were lower than control side(P<0.01).Conclusion: MT01 could down-regulate the expression levels of TLR9 and TRAF6 during orthodontic tooth movement and eventually resists the inflammation during the tooth movement.

Key words: MT01, tooth movement, tumor necrosis factor receptor associated factor 6, toll like receptors 9, interleukin-6, polymerase chain reaction

中图分类号: 

  • R783.5