吉林大学学报(医学版) ›› 2020, Vol. 46 ›› Issue (01): 61-65.doi: 10.13481/j.1671-587x.20200111

• 基础研究 • 上一篇    下一篇

食源性原花青素对SH-SY5Y细胞生长的抑制作用及其机制

钟越1, 李海超2, 冯馨锐2, 崔雨舒2, 郑中华2, 王玮瑶2, 齐玲2,3   

  1. 1. 吉林医药学院管理学院, 吉林 吉林 132013;
    2. 吉林医药学院病理生理学教研室, 吉林 吉林 132013;
    3. 广州医科大学附属第六医院 广东省清远市人民医院, 广东 清远 511518
  • 收稿日期:2019-01-21 出版日期:2020-01-28 发布日期:2020-02-03
  • 通讯作者: 齐玲,教授,硕士研究生导师(Tel:0432-64560465,E-mail:qiling1718@163.com) E-mail:qiling1718@163.com
  • 作者简介:钟越(1973-),女,吉林省吉林市人,教授,医学硕士,主要从事抗肿瘤药物方面的研究。
  • 基金资助:
    吉林省科技厅自然科学基金资助课题(20160101195JC,20180101306JC,20190101006JH);吉林省卫健委科研项目资助课题(2016J102)

Inhibitory effect of foodborne procyanidins on growth of SH-SY5Y cells and its mechanism

ZHONG Yue1, LI Haichao2, FENG Xinrui2, CUI Yushu2, ZHENG Zhonghua2, WANG Weiyao2, QI Ling2,3   

  1. 1. Faculty of Management, Jilin Medical University, Jilin 132013, China;
    2. Department of Pathophysiology, Jilin Medical University, Jilin 132013, China;
    3. Sixth Affiliated Hospital, Guangzhou Medical University, Qingyuan People's Hospital, Guangdong Province, Qingyuan 511518, China
  • Received:2019-01-21 Online:2020-01-28 Published:2020-02-03

摘要: 目的:探讨食源性原花青素对人神经母细胞瘤SH-SY5Y细胞生长的抑制作用,并阐明其作用机制。方法:培养SH-SY5Y细胞,将细胞分为对照组及10、20和40mg·L-1食源性原花青素组,各组细胞中加入含不同浓度(0、10、20和40mg·L-1)食源性原花青素的培养基,采用噻唑蓝(MTT)法检测药物作用24、48和72h时SH-SY5Y细胞增殖率,流式细胞术检测药物作用72 h时不同细胞周期SH-SY5Y细胞百分率,Annexin Ⅴ凋亡试剂盒检测药物作用72 h时SH-SY5Y细胞的凋亡率。结果:与对照组比较,各时间点10、20和40mg·L-1食源性原花青素组SH-SY5Y细胞增殖率均降低,作用48和72h时20mg·L-1食源性原花青素组细胞增殖率差异有统计学意义(P<0.05或P<0.01),作用24、48和72h时40mg·L-1食源性原花青素组细胞增殖率差异有统计学意义(P<0.01)。与对照组比较,40mg·L-1组食源性原花青素组细胞中G0/G1期细胞百分率升高(P<0.01),G2/M期细胞百分率降低(P<0.01)。与对照组比较,10、20和40mg·L-1食源性原花青素组细胞凋亡率均明显升高(P<0.01)。结论:食源性原花青素可抑制人神经母细胞瘤SH-SY5Y细胞的生长,其机制主要是阻滞细胞周期和诱导细胞凋亡。

关键词: 食源性原花青素, SH-SY5Y细胞, 细胞周期, 细胞凋亡

Abstract: Objective: To investigate the inhibitory effect of foodborne procyanidins on the growth of human neuroblastoma SH-SY5Y cells, and to elucidate its mechanism. Methods: The SH-SY5Y cells were cultured and divided into control group, 10 mg·L-1 foodborne procyanidins group, 20 mg·L-1 foodborne procyanidins group and 40 mg·L-1 foodborne procyanidins group;the medium containing different concentrations (0, 10, 20 and 40 mg·L-1) of foodborne procyanidins was added into each group. The proliferation rates of SH-SY5Y cells were measured by MTT method at 24, 48 and 72 h after the drug treatment. Flow cytometry was used to detect the cell cycle of SH-SY5Y cells at 72 h after the drug treatment, and the apoptotic rate of SH-SY5Y cells was detected by Annexin Ⅴ apoptotic assay kit at 72 h after the drug treatment. Results: Compared with control group, the proliferation rates of SH-SY5Y cells at 24, 48 and 72 h in 10, 20 and 40 mg·L-1 foodborne procyanidins groups were decreased; there were significant differences at 48 and 72 hours in 20 mg·L-1 foodborne procyanidins group (P<0.05 or P<0.01);there were also significant differences at 24, 48 and 72 h in 40 mg·L-1 foodborne procyanidins group (P<0.01). Compared with control group, the percentage of cells in G0/G1 phase in 40 mg·L-1 foodborne procyanidins group were increased(P<0.01) and the percentage of cells in G2/M phase were decreased (P<0.01). Compared with control group, the apoptotic rates of cells in 10, 20 and 40 mg·L-1 foodborne procyanidins groups were increased significantly (P<0.01). Conclusion: Foodborne procyanidins can inhibit the growth of human neuroblastoma SH-SY5Y cells, and its mechanism is mainly to block the cell cycle and induce the apoptosis.

Key words: foodborne procyanidins, SH-SY5Y cells, cell cycle, apoptosis

中图分类号: 

  • R739.4