关键词: 天然, 抗白血病活性, 疾病模型, 动物, 小鼠

Abstract: To Study the antileukemic activity of donor-derived NK c ell in mice in vivo. Methods CB6F₁H-2b/d mouse model of EL9611 (H-2d) erythroleukemia was developed by injection of EL9611 (H-2d) cells in tail vein. 30 mice with erythroleukemia were randomly divided into 5 groups (six mice in each group). Experimental groups(2 groups): 2×10⁵ and 5×10⁵ NK cells of C₅₇BL/6H-2b were injected into mice after injected EL9611(H-2d) cells for 5 d in the first and the second experimental groups. Control groups（3 groups）: the first group was the one without treatment; the second one was the Ara-c-treated group which Ara-c were injected into mouse at 50 mg•kg^-1×6 d followed by infusion of EL9611(H-2d) for 5 d; the third one was syngeneic NK cell treated group, CB6F₁H-2b/d NK cells(1×106) were injected into CB6F₁H-2b/d mice after injected EL9611(H-2d) cells for 5 d. The effect was assessed by blood routine test, survival time, body weight, and histopathology in the recipients. Results ①There was no significant difference of life span between two experimental groups (P>0.05). The survival time of the second control group and experimental groups was much longer than that of the first control group (P<0.01); the survival time of the second of control group was much longer than that of experimental groups (P<0.01); the survival time of experimental groups was much longer than that of the third control group (P<0.01). Conclusion NK cell (H-2b) can delay erythroleukemia (EL9611, H-2d) CB6F1H-2b/d death time in erythroleukemia (EL9611, H-2d) CB6F₁H-2b/d model.

Key words: natural, antileukemic activity, disease model, animal, mice