吉林大学学报(医学版)

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壳层隔绝纳米粒子增强光谱方法检测乳腺导管内癌组织的生物学特性

张海鹏1,付彤2,宋东2,吴迪2,范志民2,郑超2,韩冰2   

  1. 1.吉林大学第一医院产科,吉林 长春 130021;2.吉林大学第一医院乳腺外科,吉林 长春 130021
  • 收稿日期:2013-09-18 出版日期:2014-03-28 发布日期:2014-05-28
  • 通讯作者: 韩 冰(Tel:0431-81875672,E-mail:yintian77@126.com) E-mail:yintian77@126.com
  • 作者简介: 韩 冰(Tel:0431-81875672,E-mail:yintian77@126.com)
  • 基金资助:

    国家自然科学基金青年基金资助课题(81202078);吉林省科技厅科技发展计划项目青年基金资助课题(20130522030JH);吉林省科技厅科技发展计划项目资助课题(201015155)

Biological characteristics of breast ductal carcinoma in situ detected by shell-isolated nanoparticle-enhanced Raman spectroscopy

ZHANG Hai-peng1,FU Tong2,SONG Dong2,WU Di2,FAN Zhi-min2,ZHENG Chao2,HAN Bing2   

  1. 1.Department of Obstetrics,First Hospital,Jilin University,Changchun 130021,China;2.Department of Breast Surgery,First Hospital,Jilin University,Changchun 130021,China
  • Received:2013-09-18 Online:2014-03-28 Published:2014-05-28

摘要:

目的:采用壳层隔绝纳米粒子增强光谱(SHINERS)检测乳腺导管内癌(DCIS)组织和正常乳腺组织,探讨乳腺DCIS的生物学特征和鉴别方法。方法:收集行乳腺外科手术的17例患者的乳腺组织冰冻切片,包括乳腺DCIS组织8例,正常乳腺组织9例。冰冻切片解冻后先行普通拉曼光谱检测,加入壳层隔绝纳米粒子(SHINs)后再次检测。共收集223个拉曼光谱和231个SHINSERS,所有光谱均进行基线修正拟合,将所有光谱用Adjacent-Averaging算法进行15点平滑。结果:正常乳腺组织特征峰出现在1 090、1 157、1 262、1 300、1 442、1 658、1 745和1 874 cm-1;在加入SHINs后,少数特征峰的峰位出现2~3 cm-1位移,其中1 090、1 157 cm-1相对强度明显增加,出现1 496 cm-1特征峰。普通拉曼光谱可见乳腺DCIS组织光谱有多个核酸特征峰(包括878、1 086和1 157 cm-1);加入SHINs后,明显出现1 004、1 157、1 526和1 658 cm-1相对强度增加,脂类的特征峰1 745 cm-1和1 442 cm-1左右为C=O和CH2伸缩振动,乳腺DCIS组织相对于正常组织表现出2~3 cm-1蓝移;核酸的特征峰1 090 cm-1蓝移至1 086 cm-1。结论:拉曼光谱能够发现乳腺DCIS组织DNA和蛋白质与正常乳腺组织之间的差异,SHINERS可以用来区分乳腺DCIS和正常组织。

关键词:  , 乳腺肿瘤, 拉曼光谱, 表面增强拉曼光谱, 导管内癌

Abstract:

Objective To identify normal breast tissues and breast ductal carcinoma in situ (DCIS) tissue by shell-isolated nanoparticle-enhanced Raman spectroscopy (SHINERS),and to explore the biological characteristics of DCIS and the identification method.Methods The frozen section of breast tissue from 17 patients underwent surgical resection were obtained.9 cases of normal breast tissue and 8 cases of DCIS tissue  were detected by Raman spectroscopy first and then SHINERS technique was utilized after adding shell-isolated nanoparticles(SHINs).A total of 223 Raman spectra and 231 SHINERS  were obtained.All the spectra were dealt with baseline corrected by fitting and subtracting a third-order polynomial and then smoothed with a 15-point Adjacent-Averaging.Results  The characteristic peak of normal breast tissue  appeared at 1 090,1 157,1 262,1 300,1 442,1 658,1 745,and  1 874 cm-1.After adding SHINs,some peaks shifted  2-3 cm-1, and the relative strength of 1 090 and 1 157 cm-1 was significantly increased,and the characteristic peak at 1 496 cm-1  appeared.The Raman spectrocopy showed there were more nucleic acid characteristic peaks (including 878,1 086,and 1 157 cm-1) in breast DCIS tissue;after adding SHINs,the relative strength of 1 004,1 157,1 526,and 1 658 cm-1 was increased,the characteristics peak of lipid around 1 745 cm-1 and 1 442 cm-1 stemed   from the C=O and CH2 stretching vibration.Compared with normal breast tissue,DCIS tissue showed a blue shift of 2-3 cm-1.The characteristic peak of nucleic acid had the blue shift from 1 086 cm-1 to 1 090 cm-1.Conclusion Raman spectra can discover the differences of the characteristic peaks of DNA and protein between DCIS and normal breast tissues,and SHINERS  can distinguish  breast DCIS  tissue from  normal tissue successfully.

Key words: breast neoplasms, Raman spectroscopy, surface enhanced Raman spectroscopy, ductal carcinoma in situ

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