四物汤对小鼠化疗所致贫血的恢复作用及其机制

doi:10.13481/j.1671-587x.20180601

摘要/Abstract

摘要： 目的：探讨四物汤对小鼠化疗所致贫血的恢复作用，并阐明其作用机制。方法：24只小鼠随机分为对照组（n=8，不给药）、模型组（n=8，给予5-氟尿嘧啶）和四物汤组（n=8，给予5-氟尿嘧啶+四物汤）。于给药15 d后，眼静脉取血，显微镜下观察红细胞形态和数量；应用全自动血细胞计数仪检测3组小鼠外周血红细胞数和血红蛋白水平；采用双染标记法对红细胞表面标志性抗原进行抗体结合并染色，采用流式细胞术检测3组小鼠外周血和脾脏标记的红细胞数。结果：与对照组比较，模型组小鼠外周血红细胞数明显减少（P<0.05），细胞皱缩；与模型组比较，四物汤组小鼠外周血红细胞数量明显增加（P<0.05），细胞变圆。与对照组比较，模型组小鼠血红蛋白水平明显降低（P<0.05）；与模型组比较，四物汤组小鼠血红蛋白水平明显升高（P<0.01）。流式细胞术检测，与对照组比较，模型组小鼠外周血CD71/Ter119表达水平明显降低（P<0.01），各象限红细胞数量降低（P<0.01）；与模型组比较，四物汤组小鼠外周血CD71/Ter119表达水平明显升高（P<0.01），各象限红细胞数量升高（P<0.01）；与对照组比较，模型组小鼠脾脏血CD71/Ter119表达水平明显降低（P<0.01），各象限红细胞数量降低（P<0.01）；与模型组比较，四物汤组小鼠脾脏血CD71/Ter119表达水平明显升高（P<0.01），UL象限红细胞数量降低（P<0.01），UR和LR象限红细胞数量升高（P<0.01）。结论：四物汤可以促进小鼠化疗贫血损伤的恢复，促进红细胞数量增加，改善细胞形态表现，提升外周血血红蛋白水平，促进外周血和脾脏红细胞表面标志性抗原表达量增加。

关键词: 四物汤, 红细胞, 血红蛋白, 表面标志性抗原CD71/Ter119

Abstract: Objective: To study the recovery effect of Siwutang on the anemia induced by chemotherapy in the mice, and to clarify its mechanism.Methods: A total of 24 mice were randomly divided into control group(n=8,not given any drugs),model group(n=8,given 5-fluorouracil),Siwutang group(n=8,given 5-fluorouracil +Siwutang). After administration for 15 d,the peripheral blood of mice was taken,and the morphology and the number of red cells were observed under microscope. The number of peripheral blood red cells and the levels of hemoglobin of the mice in various groups were calculated by full-automatic hemocytometer; Double staining was used to mark the surface antigens of red cells,then antibody binding and staining were performed.Flow cytometry was used to detect the number of peripheral blood-labelled and spleen-labelled red cells.Results: Compared with control group, the number of red cells in peripheral blood of the mice in model group was significantly decreased(P<0.05),and the cells shrinkaged. Compared with model group, the number of red cells in peripheral blood of the mice in Siwutang group was significantly increased(P<0.05) and the cells showed rounding. Compared with control group, the level of hemoglobin in peripheral blood of the mice in model group was significantly decreased (P<0.05); compared with model group, the level of hemoglobin in peripheral blood of the mice in Siwutang group was significantly increased (P<0.01). The flow cytometry results showed that compared with control group, the expression level of CD71/Ter119 in peripheral blood of the mice in model group was significantly decreased (P<0.01),and the number of red cells in various quadrants was decreased(P<0.01); compared with model group, the expression level of CD71/Ter119 in peripheral blood of the mice in Siwutang group was significantly increased (P<0.01),and the number of red cells in various quadrants was increased(P<0.01);compared with control group, the expression level of CD71/Ter119 in spleen blood of the mice in model group was significantly decreased (P<0.01),and the number of cells in various quadrants was decreased(P<0.01); compared with model group, the expression level of CD71/Ter119 in spleen blood of the mice in Siwutang group was significantly increased (P<0.01),the number of red cells in UL quadrant was decreased (P<0.01),and the number of red cells in UR and LR quadrants was increased(P<0.01).Conclusion: Siwutang can promote the recovery of anemia induced by chemotherapy in the mice, increase the number of red cells,improve the morphology of red blood cells,increase the level of hemoglobin in peripheral blood, and increase the expression levels of marker antigens on the surface of red cells in the peripheral blood and spleen.

Key words: Siwutang, red blood cells, hemoglobin, surface marker antigen CD71/Ter119

中图分类号:

R556

刘丹, 杨晓波, 王颖, 于丽红, 石焱, 张英杰, 周琳, 石磊. 四物汤对小鼠化疗所致贫血的恢复作用及其机制[J]. 吉林大学学报(医学版), 2018, 44(06): 1115-1119.

LIU Dan, YANG Xiaobo, WANG Ying, YU Lihong, SHI Yan, ZHANG Yingjie, ZHOU Lin, SHI Lei. Effect of Siwutang on recovery of anemia induced by chemotherapy in mice and its mechanism[J]. Journal of Jilin University(Medicine Edition), 2018, 44(06): 1115-1119.

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