吉林大学学报(医学版) ›› 2023, Vol. 49 ›› Issue (4): 905-912.doi: 10.13481/j.1671-587X.20230411

• 基础研究 • 上一篇    下一篇

大鼠神经病理性疼痛模型的建立及其引起抑郁样状态伴失眠的机制

姜政男1,2,张溢珈1,刘臻1,陈云飞1()   

  1. 1.上海中医药大学附属岳阳中西医结合医院针灸科,上海 200437
    2.上海市宝山区淞南镇 社区卫生服务中心康复科,上海 200441
  • 收稿日期:2023-01-13 出版日期:2023-07-28 发布日期:2023-07-26
  • 通讯作者: 陈云飞 E-mail:icyf1968@163.com
  • 作者简介:姜政男(1989-),男,吉林省延吉市人,主治医师,在读硕士研究生,主要从事针灸治疗失眠基础方面的研究。
  • 基金资助:
    国家自然科学基金青年科学基金项目(81904300);上海市科学技术委员会科技创新行动计划自然科学基金项目(21ZR1464300)

Construction of neuropathic pain model in rats and its mechanism of depressive state complicated with insomnia

Zhengnan JIANG1,2,Yijia ZHANG1,Zhen LIU1,Yunfei CHEN1()   

  1. 1.Department of Acupuncture and Moxibustion,Affiliated Yueyang Hospital of Integrated Traditional Chinese and Western Medicine,Shanghai University of Traditional Chinese Medicine,Shanghai 200437,China
    2.Department of Rehabilitation,Community Healthcare Center,Songnan Town,Baoshan District,Shanghai City,Shanghai 200441,China
  • Received:2023-01-13 Online:2023-07-28 Published:2023-07-26
  • Contact: Yunfei CHEN E-mail:icyf1968@163.com

摘要:

目的 建立神经病理性疼痛致抑郁伴失眠大鼠模型,阐明慢性压迫性损伤(CCI)对大鼠情绪和睡眠-觉醒行为的影响。 方法 16只雄性SD大鼠随机分为假手术组和CCI组,每组8只。CCI组大鼠采用4-0丝线结扎右侧坐骨神经干,结扎以维持坐骨神经外膜供血为度,假手术组大鼠仅将坐骨神经干进行暴露和分离,不进行结扎,术后每周检测2组大鼠机械刺激缩足反射阈值(PWMT)评估大鼠痛阈变化。糖水偏好实验检测2组大鼠糖水偏好百分率,悬尾实验检测2组大鼠悬尾静止时间,旷场实验检测2组大鼠水平运动总距离,评价大鼠是否存在抑郁样行为。通过植入式脑电/肌电评价大鼠睡眠-觉醒行为。 结果 造模后第1~4周,与假手术组比较,CCI组大鼠PWMT和糖水偏好百分率明显降低(P<0.01),悬尾静止时间明显延长(P<0.01),水平运动总距离明显缩短(P<0.01)。造模后第2~4周,与假手术组比较,CCI组大鼠主观黑夜觉醒量明显增加(P<0.01),非快速眼动(NREM)睡眠量明显减少(P<0.01),快速动眼(REM)睡眠量明显增加(P<0.05或P<0.01);与假手术组比较,CCI组大鼠主观黑夜觉醒累积量和REM睡眠累积量明显增加(P<0.05或P<0.01),NREM睡眠累积量明显减少(P<0.01)。 结论 CCI大鼠存在抑郁状态伴失眠,且症状呈慢性化趋势维持至造模后第4周,该模型可用于抑郁状态伴失眠的长期效应观察和相关机制研究。

关键词: 神经病理性疼痛, 失眠, 抑郁状态, 疾病模型,动物

Abstract:

Objective:To establish the rat model of depressive state complicated with insomnia caused by neuropathic pain, and to clarify the effect of chronic constrict injury(CCI) on the mood and sleep-wake behaviors of the rats. Methods Sixteen male SD rats were divided into sham operation group (n=8) and CCI group (n=8), respectively. In CCI group, 4-0 silk thread was used to ligate the right sciatic nerve trunk of the rats, and the intensity was to maintain the blood supply of the sciatic nerve adventitia. In sham operation group, the sciatic nerve trunk of the rats was exposed and separated without ligation. The paw withdraw mechanical threshold (PWMT) of rats in two groups was measured weekly after operation to evaluate the pain threshold changes. Sucrose preference test was used to detect the percentages of sucrose preference of rats in two groups; tail suspension test was used to detect the immobility time in tail suspension of rats in two groups;open field test was used to detect the total distance of horizontal movement of rats in two groups;whether or not the depression-like behaviors of the rats existed were assessed;the implantable electro encepha logram/myoelectricity was used to analyze the sleep-wake behaviors of the rats. Results Compared with sham operation group, during 1-4 weeks after modeling, the PWMT and the percentage of sucrose preference of the rats in CCI group were significantly decreased(P<0.01), the immobility time in tail suspension of the rats was increased (P<0.01), and the total distance of horizontal movement of the rats was decreased (P<0.01). Compared with sham operation group,during 2-4 weeks after modeling,the subjective night wake amount of the rats in model group was increased significantly (P<0.01),the non-rapid eye movement (NREM) sleep amount was decreased (P<0.01), and the rapid eye movement (REM) sleep amount was increased (P<0.05 or P<0.01).Compared with sham operation group,the subjective night cumulative amount of sleep-wake states and the REM sleep cumulative amount of the rats in CCI group were increased (P<0.05 or P<0.01),and the NREM sleep cumulative amount was decreased(P<0.01). Conclusion The CCI rats have the depressive state complicated with insomnia,and these symptoms show a chronic trend and persist until the 4th week after modeling; this model can be used for observation of long-term effect and studying of related mechanism of the depressive state comlicated with insomia.

Key words: Neuropathic pain, Insomnia, Depression, Disease model,animal

中图分类号: 

  • R338