Galectin-3及整合素ανβ3 mRNA在脑胶质瘤细胞中的表达及其意义

J4 ›› 2012, Vol. 38 ›› Issue (5): 973-976.

Galectin-3及整合素ανβ3 mRNA在脑胶质瘤细胞中的表达及其意义

李云超1|邱虹1|阚志生1|韩依轩1|陈广1|于向东1|庄雅娟2|孙向辉3

LI Yun-chao¹|QIU Hong¹|KAN Zhi-sheng¹|HAN Yi-xuan¹|CHEN Guang¹|YU Xiang-dong¹|ZHUANG Ya-juan²|SUN Xiang-hui³

收稿日期:2012-05-08 出版日期:2012-09-28 发布日期:2012-09-28
通讯作者: 阚志生(Tel:0315-3726240,E-mail:liyunchao_lyc@hotmail.com) E-mail:liyunchao_lyc@hotmail.com
作者简介:李云超（1976-）|男|河北省唐山市人|主治医师|医学硕士|主要从事神经外科的基础及临床研究。
基金资助:
中国煤炭工业协会基金资助课题 (MTKJ2010-091)

Expressions |of Galectin-3 and integrin ανβ3 mRNA in glioma cells and their significances

1.Department of Neurosurgery|Affiliated Kailuan Hospital|Hebei United University| Tangshan 063000|China；2.Department of Pediatrics|Affiliated Hospital|Hebei United University|Tangshan 063000|China；3.Department of Internal Medicine|Qinggang Chinese Medicine Hospital|Heilongjiang Province,Qinggang 151600|China

Received:2012-05-08 Online:2012-09-28 Published:2012-09-28

摘要/Abstract

摘要：

目的：探讨半乳糖凝集素-3（Galectin-3）及整合素ανβ3 mRNA在胶质瘤细胞中的表达及二者在胶质瘤的发生及恶性进展中的作用。方法：采用原位杂交法检测5例正常脑组织及40例不同级别胶质瘤中Galectin-3 mRNA、整合素ανβ3 mRNA的表达情况，并分析二者之间的关系。结果：正常脑组织中Galectin-3 mRNA、整合素ανβ3 mRNA的表达均为阴性。Galectin-3 mRNA主要表达于脑胶质瘤细胞的胞浆中,在23例Ⅰ-Ⅱ级胶质瘤标本中阳性2例、弱阳性4例、阴性17例，阳性表达率为29.09%(6/23)，阳性指数(LI)值为(4.80±3.22)%;在17例Ⅲ-Ⅳ级胶质瘤标本中强阳性4例、阳性6例、弱阳性1例、阴性6例，阳性表达率为64.71%(11/17)，LI值为(27.30±22.72)%，Ⅰ-Ⅱ级胶质瘤Galectin-3 mRNA阳性表达率低于Ⅲ-Ⅳ级（P<0.05）。整合素ανβ3 mRNA主要表达于脑胶质瘤细胞的胞浆中，在23例Ⅰ-Ⅱ级胶质瘤标本中阳性3例、弱阳性4例、阴性16例，阳性表达率为34.43%(7/23)，LI值为(5.35±3.79)%；在17例Ⅲ-Ⅳ级胶质瘤标本中强阳性4例、阳性6例、弱阳性3例、阴性4例，阳性表达率为76.47%(13/17)，LI值为(29.24±21.10)%，Ⅰ-Ⅱ级胶质瘤整合素ανβ3 mRNA 阳性表达率低于Ⅲ-Ⅳ级（P<0.05）。直线相关分析结果显示，Galectin-3 mRNA与整合素ανβ3 mRNA的阳性细胞表达率呈正相关（r=0.718，P<0.05）。结论：Galectin-3 mRNA、整合素ανβ3 mRNA的表达在Ⅰ-Ⅱ级胶质瘤组明显低于Ⅲ-Ⅳ级胶质瘤组，提示Galectin-3及整合素ανβ3 mRNA检测可作为从分子水平判定人脑胶质瘤恶性生物学行为的指标。

关键词: 胶质瘤；Galectin-3；整合素ανβ3;原位杂交

Abstract:

Objective To study the expressions of galactose Galectin-3 and integrin ανβ3 mRNA in glioma cells and their roles in the occurrence and the malignancy progress of glioma.Methods In situ hybridization was used to detect the expressions of Galectin-3 and integrin ανβ3 mRNA in 5 normal brain tissues and 40 patients with different grades gliomas , and the relationship between them was analyed. Results The expressions of Galectin-3 and integrin ανβ3 mRNA in normal brain tissue were negative.Galectin-3 mRNA mainly expressed in the cytoplasm and membrane of tumor cells.In 23 cases ofⅠ, Ⅱ grade glioma specimens, 2 cases were positive,4 cases were weakly positive and 17 cases were negative, the positive rate was 29.09%(6/23), LI value was (4.80±3.22)%;in 17 cases of Ⅲ, Ⅳ grade glioma specimens,4 cases were strongly positive,6 cases were positive ，1 case was weakly positive and 6 cases were negative，the positive rate was 64.71%(11/17), LI value was (27.30±22.72)%; there was significant difference in the positive rate betweenⅠ，Ⅱ grade and Ⅲ, Ⅳgrade glioma(P<0.05). The integrin ανβ3 mRNA mainly expressed in the cytoplasm and membrane of tumor cells. In
23 cases of Ⅰ, Ⅱ grade glioma specimens 3 cases were positive expressin of integrin αυβ3,4 cases were weakly positive, 16 cases were negative, the positive rate was 34.43% (7 / 23), LI value was (5.35 ± 3.79)%; in 17 cases of Ⅲ, Ⅳ grade glioma specimens,4 cases were strongly positive, 6 cases were positive, 3 cases were weakly positive, and 4 cases were negative，the positive rate was 76.47% (13/17), and LI value was (29.24± 21.10)%; there was significant difference in the positive rate betweenⅠ，Ⅱ grade and Ⅲ, Ⅳgrade glioma(P<0.05).The linear correlation analysis showed that the expression rates of Galectin-3 and integrin ανβ3 mRNA were positively correlated (P<0.05).Conclusion The expressions of Galectin-3 and integrin ανβ3 mRNA in Ⅰ, Ⅱ grade gliomas are significantly lower than those of Ⅲ, Ⅳ grade glioma,which indicates that the detection of Galectin-3 and integrin ανβ3 mRNA can be used as an indicator to judge malignant biological behavior of human brain glioma at molecular level.

Key words: glioma, Galectin-3, integrin ανβ3, in situ hybridization

中图分类号:

李云超|邱虹|阚志生|韩依轩|陈广|于向东|庄雅娟|孙向辉. Galectin-3及整合素ανβ3 mRNA在脑胶质瘤细胞中的表达及其意义[J]. J4, 2012, 38(5): 973-976.

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