不同剂量电离辐射对小鼠脾脏调节性T细胞及TGF-β1表达的影响

J4 ›› 2011, Vol. 37 ›› Issue (3): 398-402.

不同剂量电离辐射对小鼠脾脏调节性T细胞及TGF-β1表达的影响

孟凡旭¹, 单玉兴², 董娟聪¹, 金顺子¹

（1. 吉林大学公共卫生学院卫生部放射生物学重点实验室|吉林长春 130021；2. 吉林大学第一医院骨科| 吉林长春 130021)

收稿日期:2010-11-18 出版日期:2011-05-28 发布日期:2011-05-28
通讯作者: 金顺子 E-mail:E-mail：shunzij@yahoo.com.cn
作者简介:孟凡旭（1984-）|男|河南省濮阳市人|在读医学博士|主要从事辐射免疫学机制方面的研究。
基金资助:
国家自然科学基金资助课题（30870584）

Effects of ionizing radiation with different doses on |regulatory T cells and |expression of TGF-β1 in mouse spleen


MENG Fan-Xu¹, CHAN Yu-Xin², DONG Juan-Cong¹, JIN Shun-Zi¹

（1. Key Laboratory of Radiobiology,Ministry of Health,School of Public Health,Jilin University,Changchun 130021,China|2. Department of Orthopedics,First Hospital,Jilin University,Changchun 130021,China）

Received:2010-11-18 Online:2011-05-28 Published:2011-05-28

摘要/Abstract

摘要：

目的：观察不同剂量X射线照射后小鼠脾脏调节性T细胞（Treg）和转化生长因子-β1(TGF-β1)表达的变化，探讨X射线对Treg阳性率及TGF-β1表达水平的影响。方法：雄性ICR小鼠，用X.S.S.205（FZ）型固定式X射线深部治疗机进行不同剂量的全身照射（低剂量组为0.050、0.075、0.100和0.200 Gy；高剂量组为0.500、1.000、2.000、4.000 和6.000 Gy），同时设立假照组，于照射后16 h处死小鼠，取脾脏，分别采用流式细胞术、RT-PCR及Western blotting法检测Treg和TGF-β1的表达水平。结果：与假照组比较，低剂量照射组小鼠脾脏Treg阳性率无明显变化（P>0.05）；高剂量照射组，1.000 Gy照射后开始脾脏Treg阳性率明显高于假照组（P<0.05,P<0.01），并呈剂量依赖性升高。与假照组比较，小鼠脾脏细胞TGF-β1 mRNA表达水平从0.500 Gy剂量组开始升高， 2.000 Gy组达到假照组的2倍以上，但随后略有下降；在低剂量照射范围时，0.100 Gy组小鼠TGF-β1蛋白表达水平明显高于假照组(P<0.05)，而在大剂量组呈递增趋势，4.000 Gy和6.000 Gy组与假照组比较差异有统计学意义(P<0.05)。结论：在高剂量X射线诱导下小鼠脾脏Treg阳性率增高，同时TGF-β1分泌增加，这种变化可能是高、低剂量X射线诱导不同免疫效应的又一原因。

关键词: 电离辐射；调节性T细胞；转化生长因子；免疫效应

Abstract:

Abstract:Objective
To observe the changes of the regulatory T cells（Treg）and the expression of TGF-β1 in mouse spleen induced by different doses of X-ray irradiation at different time and study the effects of the X-ray on the Treg and TGF-β1. Methods The male ICR mice were irradiated with different doses of X-ray (low dose:0,0.050 Gy,0.075 Gy,0.100 Gy,0.200 Gy;high dose: 0,0.500 Gy,1.000 Gy,2.000 Gy,4.000 Gy,6.000 Gy) respectively by the XSS205 (FZ)-type stationary X-ray deep therapy machine all over the body. Then the mice were killed 16 h after irradiation and the Treg and the expression level of TGF-β1 in the mouse spleen were detected by flow cytometry,RT-PCR and Western blotting.Results Compared with sham-irradiation group,the percentage of Treg had no significant changes in mouse spleen in low dose irradiation groups (P<0.05); in high dose irradiation groups,the percentage of Treg was increased as the dose rose from 1.000 Gy to the higher levels(P< 0.05,P< 0.01),which showing a dose-dependent phenomenon. Compared with sham-irradiaton group,the level of TGF-β1 mRNA in mouse spleen had a trend to increase sustainedly as the dose was increased from 0.500 Gy to the higher doses,and then was increased to 2.000 times higher in 2 Gy group,following with a slight decrease. The level of TGF-β1 protein in mouse spleen in 0.100 Gy group was significantly higher than that in sham-irradiation group,and had an increasing tendency in high dose irradiation groups.There were significantly differences of the expression of TGF-β1 protein between 4.000 Gy group,6.000 Gy group and sham-irriadiation group(P<0.05).Conclusion The percentage of Treg is increased after induced by high dose X-ray,as well as the increasing secretion of the TGF-β1,which might be another reason for high and low dose X-rays inducing different immune responses.

Key words: ionizing radiation;regulatory T cells;transforming growth factor;immune response

中图分类号:

孟凡旭, 单玉兴, 董娟聪, 金顺子. 不同剂量电离辐射对小鼠脾脏调节性T细胞及TGF-β1表达的影响[J]. J4, 2011, 37(3): 398-402.

MENG Fan-Xu, CHAN Yu-Xin, DONG Juan-Cong, JIN Shun-Zi. Effects of ionizing radiation with different doses on |regulatory T cells and |expression of TGF-β1 in mouse spleen
[J]. J4, 2011, 37(3): 398-402.

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