关键词:  红景天苷；大鼠；肾脏；缺血再灌注损伤；热休克蛋白72；核转录因子κB

Abstract:

Abstract:Objective To study the effect of salidroside on rat renal ischemia reperfusion injury,and to clarify  its mechanism of action.Methods 54 healthy SD rats were randomly divided into nine groups:control group,ischemia-reperfusion groups by clamping 15,30,45 and 60 min,intraperitoneal injection of salidroside solution groups by clamping 15,30,45 and 60 min.The serum creatinine (Scr) in various groups were detected.The pathological changes of rat kidney tissues were observed by light microscope.The expressions of HSP72 and NF-κB in kidney tissue were determined by Western blotting and immunohistochemistry.Results Compared with ischemia-reperfusion groups,the levels of  Scr in salidroside solution groups(clamping 15,30 and 45 min) were significantly decreased (P<0.01),the  pathological damage was significantly reduced,and the expressions of HSP72 and NF-κB in kidney tissue were also decreased significantly.Compared with  control group,the  Scr levels in salidroside groups were increased slightly,especially in  clamping 60 min group,it was increased significantly(P<0.01),but in  clamping 15,30 and 45 min groups, the changes were not obvious.Conclusion Application of salidroside can significantly reduce renal ischemia-reperfusion injury in kidney tissue,the mechanisms may be related to  enhancing the antioxidant capacity of the kidneys,reducing inflammatory cell infiltration  and apoptosis in kidney tissue and inhibiting  the HSP72 and NF-κB expressions in kidney tissue.

Key words: Abstract:Objective To study the effect of salidroside on rat renal ischemia reperfusion injury,and to clarify