TGFβ1/Smads信号通路相关蛋白在乳腺癌组织中的表达及其临床意义

J4 ›› 2012, Vol. 38 ›› Issue (1): 93-95.

TGFβ1/Smads信号通路相关蛋白在乳腺癌组织中的表达及其临床意义

孙凤丹¹|张金子^1,2|崔演³|程志芬¹|代博²|玄延花¹

1.延边大学基础医学院病理学教研部|吉林延吉133002；2.吉林博华医院内科|吉林吉林 132013；3.延边大学附属医院肿瘤科|吉林延吉 133000

收稿日期:2011-10-10 出版日期:2012-01-28 发布日期:2012-01-13
通讯作者: 玄延花（Tel: 0433-2435059，E-mail:xuanyh1@ybu.edu.cn） E-mail:xuanyh1@ybu.edu.cn
作者简介:孙凤丹（1964-）|女|吉林省九台市人|副教授|医学硕士|主要从事肿瘤病理学研究。
基金资助:
国家自然科学基金资助课题（30560047，81160315)

Expressions of TGFβ1/Smads proteins in breast cancer tissues and their clinical significances

SUN Feng-dan¹,ZHANG Jin-zi^1|2|CUI Yan³,CHENG Zhi-fen¹|DAI Bo²,XUAN Yan-hua¹

1.Department of Pathology|School of Basic |Medicine|Yanbian University,Yanji 133002| China; 2.Department of Medicine|Jilin Bohua Hospital|Jilin 132013|China;3.Department of Oncology,Affiliated Hospital,Yanbian University,Yanji 133000|China

Received:2011-10-10 Online:2012-01-28 Published:2012-01-13

摘要/Abstract

摘要：

目的：研究TGFβ1/Smads信号通路相关蛋白在乳腺癌组织中的表达，阐明其与乳腺癌临床病理学特征的关系。方法：采用免疫组化EnVision方法检测121例乳腺导管癌和21例正常乳腺导管上皮组织芯片中TGFβ1、TGFβRⅠ、 TGFβRⅡ、Smad2/3和Smad4蛋白的表达水平，分析其与乳腺癌临床病理学特征的关联性。结果：免疫组化染色，TGFβ1、TGFβRⅠ、Smad2/3和TGFβRⅡ在乳腺癌组织均呈高表达，Smad4在乳腺癌组织表达水平明显低于正常导管上皮中的表达水平(P<0.01）；≤55岁乳腺癌患者TGFβ1表达水平明显高于>55岁患者（P<0.05）；孕激素受体(PR)阴性乳腺癌患者TGFβ1表达水平明显高于PR阳性患者（P<0.05），未发生淋巴结转移乳腺癌患者TGFβ1、TGFβRⅠ和TGFβ RⅡ的表达水平明显高于淋巴结转移患者（P<0.05或P<0.01）；不同临床分期乳腺癌患者TGFβRⅡ和Smad2/3表达水平具有明显差异，Ⅱa、Ⅱb和Ⅲa期乳腺癌患者TGFβRⅡ表达水平明显低于0期（P<0.01），Ⅰ期和Ⅱb期乳腺癌患者Smad2/3表达明显低于在0期（P<0.01）。TGFβ1表达水平分别与TGFβRⅠ和TGFβRⅡ表达水平呈正相关关系（r=0.46,P<0.01；r=0.44,P<0.01）。结论：TGFβ1/Smads信号通路相关蛋白的高表达可以作为乳腺癌的辅助诊断指标，提示TGFβ1/Smads信号通路相关蛋白检测可作为判定乳腺癌预后的指标。

关键词: TGFβ1/Smads；乳腺肿瘤；免疫组织化学

Abstract:

Abstract:Objective To investigate the expressions of TGFβ1/Smads signaling pathway proteins in breast cancer tissues and clarify their relationship with the clinicopathological characteristics of breast cancer. Methods The expressions of TGFβ
1,TGFβRⅠ,TGFβRⅡ,Smad2/3 and Smad4 proteins in 121 cases of breast ductal carcinoma and 21 cases of normal breast ductal epithelium tissues were detected in tissue microarray by immunohistochemistry EnVision method,and the relationship between their expressions and clinicopathological characteristics of breast cancer was analyzed. Results The immunohistochemical staining results showed that the expressions of TGFβ1,TGFβRⅠ,TGFβRⅡ and Smad2/3 in breast cancer tissues were higher;and the expression of Smad4 in breast cancer tissues was lower than that in normal ductal epithelium tissue（P<0.01）. The expression level of TGFβ1 in less than 55 years of age patients was significantly higher than that in over 55 years of patients（P<0.05）;and the TGFβ1 expression in patients with breast cancer in PR negative group was significantly higher than that in positive group（P<0.05）. The expressions of TGFβ1,TGFβRⅠ and TGFβRⅡ in patients without lymph node metastasis were significantly higher than those in patients with metastasis（P<0.05 or P<0.01）. The expressions of TGFβRⅡ and Smad2/3 had significant differences between patients with different clinical stages. The expressions of TGFβRⅡ in the patients with breast cancer in stage Ⅱa,Ⅱb and Ⅲa were lower than that in stage 0 (P<0.01).The Smad2/3 expression in the patients with breast cancer in stageⅠ and Ⅱb were lower than that in stage 0(P<0.01). The expression of TGFβ1 was positively correlated to the expressions of TGFβRⅠand TGFβRⅡ（r=0.46,P<0.01；r=0.44,P<0.01）. Conclusion High expression of TGFβ1/Smads proteins might be helpful markers for the diagnosis of breast cancer.It indicates that TGFβ1/S
mads proteins can be used as indicators in judging the prognosis of breast cancer.

Key words: TGFβ1/Smads;breast neoplasms;immunohistochemistry

中图分类号:

孙凤丹|张金子|崔演|程志芬|代博|玄延花. TGFβ1/Smads信号通路相关蛋白在乳腺癌组织中的表达及其临床意义[J]. J4, 2012, 38(1): 93-95.

SUN Feng-dan,ZHANG Jin-zi|CUI Yan,CHENG Zhi-fen|DAI Bo,XUAN Yan-hua. Expressions of TGFβ1/Smads proteins in breast cancer tissues and their clinical significances[J]. J4, 2012, 38(1): 93-95.

[1]	周颖,高孟飞,朱慧勇. 间充质干细胞干性维持信号通路的研究进展[J]. 吉林大学学报(医学版), 2013, 39(3): 638-642.
[2]	李红，陈永浩，龚红君. 右美托咪定对肾癌根治术患者围术期炎性细胞因子和肾功能的影响[J]. 吉林大学学报(医学版), 2013, 39(3): 588-591.
[3]	李海波，刘行，孙美花,梁建民. 孕期至幼年期低水平铅暴露仔鼠海马锌离子平衡的变化及其发育期海马神经元的损伤[J]. 吉林大学学报(医学版), 2013, 39(3): 521-528.
[4]	谢波，沈梅，叶文鑫，舒彦，梁臻，张心男. 依帕司他联合行为疗法治疗女性糖尿病膀胱的临床疗效评价[J]. 吉林大学学报(医学版), 2013, 39(3): 597-600.
[5]	李辉,赵丽晶,杜宇,杨铁铮，李国峰,陈亮,支晨阳,李娜,周建华. 结肠安栓联合舒血宁对溃疡性结肠炎大鼠的治疗作用及其机制[J]. 吉林大学学报(医学版), 2013, 39(3): 488-493.
[6]	张洪长，王恩鹏，陈新，唐燕，王洪鑫，王炫策，许新. 人参皂苷Re对UVC辐射损伤细胞的保护作用[J]. 吉林大学学报(医学版), 2013, 39(3): 507-511.
[7]	武国东，王素侠，及志勇，张舒岩. 急性心力衰竭大鼠B型钠尿肽水平与肝功能的关系[J]. 吉林大学学报(医学版), 2013, 39(3): 517-520.
[8]	陈宣男，全首祯. NDM1基因常规PCR与荧光定量PCR检测方法的建立及其检测结果比较[J]. 吉林大学学报(医学版), 2013, 39(3): 625-629.
[9]	周超熙,于滨,赵斌,马志强,赵文河,于跃明. 直肠癌环周切缘病理学检测判断中低位直肠癌患者预后的临床价值[J]. 吉林大学学报(医学版), 2013, 39(3): 601-604.
[10]	周广宇，韩雪梅，金玲. 神经精神狼疮患者影像学表现与自身抗体水平和预后的关系[J]. 吉林大学学报(医学版), 2013, 39(3): 609-614.
[11]	周建英，田勇韩青,贺庆,程凯亮,李幼琼. 影像学三维重建技术在寰椎立体模型建立及性别鉴定中的应用[J]. 吉林大学学报(医学版), 2013, 39(3): 605-608.
[12]	沈煜，沈惠良，方秀统. 老年股骨颈骨折患者关节置换术后死亡危险因素分析[J]. 吉林大学学报(医学版), 2013, 39(3): 574-577.
[13]	王宗谦，卢新星. 终末期肾病患者抑郁程度与生存质量各个维度之间的关系[J]. 吉林大学学报(医学版), 2013, 39(3): 615-619.
[14]	李树春，王槐. 影响美托洛尔治疗效果的药物基因组学研究进展[J]. 吉林大学学报(医学版), 2013, 39(2): 423-426.
[15]	胡源，贾卉，姜瑞，李京，刘涛涛. 不同验光方法对中年患者屈光状态的检测及其临床意义[J]. 吉林大学学报(医学版), 2013, 39(2): 363-366.