吉林大学学报(医学版) ›› 2019, Vol. 45 ›› Issue (06): 1320-1326.doi: 10.13481/j.1671-587x.20190622

• 基础研究 • 上一篇    下一篇

YAP对非小细胞肺癌细胞增殖和迁移能力的影响

周宁1, 郭纪伟2, 代娟娟2, 李雪琳2, 席思川2, 宫凯凯2, 武艳2   

  1. 1. 滨州医学院附属医院耳鼻咽喉头颈外科, 山东 滨州 256600;
    2. 滨州医学院附属医院肿瘤研究中心, 山东 滨州 256600
  • 收稿日期:2019-01-17 出版日期:2019-12-05 发布日期:2019-12-05
  • 通讯作者: 武艳,讲师(Tel:0543-3258276,E-mail:wuyan55@126.com);宫凯凯,讲师(Tel:0543-3258276,E-mail:gongkaikai1005@163.com) E-mail:wuyan55@126.com;gongkaikai1005@163.com
  • 作者简介:周宁(1986-),男,山东省滨州市人,医师,医学硕士,主要从事肿瘤发病机制方面的研究。
  • 基金资助:
    山东省科技厅重点研发计划项目资助课题(2019GSF107099);山东省卫计委医药卫生科技发展计划项目资助课题(2017WS154);山东省科技厅自然科学基金资助课题(ZR2016HM57,ZR2017PH028,ZR2018BB024)

Effect of YAP on proliferation and migration of non-small cell lung cancer cells

ZHOU Ning1, GUO Jiwei2, DAI Juanjuan2, LI Xuelin2, XI Sichuan2, GONG Kaikai2, WU Yan2   

  1. 1. Department of Otolaryngologyand Head-Neck Surgery, Affiliated Hospital, Binzhou Medical University, Binzhou 256603, China;
    2. Cancer Research Center, Affiliated Hospital, Binzhou Medical University, Binzhou 256603, China
  • Received:2019-01-17 Online:2019-12-05 Published:2019-12-05

摘要: 目的:探讨长链非编码RNA (lncRNA) MALAT1在YAP调控的非小细胞肺癌(NSCLC)细胞增殖和迁移中的作用,并阐明其作用机制。方法:选择A549和H1299细胞系分为Scramble siRNA组(转染Scramble siRNA)、siYAP-1组(转染siYAP-1)和siYAP-2组(转染siYAP-2)。RT-PCR和Western blotting法检测各组细胞中YAP和MALAT1mRNA及YAP蛋白表达水平;在A549和H1299细胞中分别转染pcDNA3.1-YAP质粒(pcDNA3.1-YAP组)、共转染pcDNA3.1-YAP质粒和siMALAT1-2(pcDNA3.1-YAP+siMALAT1-2组)及对照质粒(pcDNA3.1-YAP+Scramble siRNA组),CCK-8法检测各组细胞增殖能力,细胞划痕实验检测各组细胞迁移能力。结果:siYAP-1组和siYAP-2组A549和H1299细胞中YAP mRNA及蛋白表达水平均明显低于Scramble siRNA组(P<0.05),siYAP-1组和siYAP-2组A549和H1299细胞中MALAT1mRNA表达水平低于Scramble siRNA组(P<0.05);与pcDNA3.1-YAP组比较,pcDNA3.1-YAP+siMALAT1-2组A549和H1299细胞增殖能力降低(P<0.05),H1299细胞迁移能力降低(P<0.05)。结论:在NSCLC细胞中,YAP通过调控lncRNA MALAT1的表达促进细胞的增殖和迁移。

关键词: YAP, MALAT1, 肺肿瘤, 细胞增殖, 迁移

Abstract: Objective: To investigate the role of long non-coding RNA(lncRNA) MALAT1 in the proliferation and migration of YAP-mediated non-small cell lung cancer (NSCLC) cells,and to elucidate its mechanism. Methods: The A549 and H1299 cell lines were selected and divided into Scramble siRNA group(transfected with Scramble siRNA),siYAP-1 group(transfected with siYAP-1)and siYAP-2 group(transfected with siYAP-2). RT-PCR and Western blotting methods were used to detect the expression levels of YAP and MALAT1 mRNA and YAP protein in the cells in variouis groups. The pcDNA3.1-YAP plasmid(pcDNA3.1-YAP group), pcDNA3.1-YAP plasmid plus siMALAT1-2 (pcDNA3.1-YAP+siMALAT1-2 group)and the control plasmid (pcDNA3.1-YAP+Scramble siRNA group)were transfected into the A549 and H1299 cells, respectively.CCK-8 method and scratch assay were used to detect the proliferation and migration abilities of the cells in various groups. Results: The expression levels of YAP mRNA and protein in the A549 and H1299 cells in siYAP-1 and siYAP-2 groups were significantly lower than those in Scramble siRNA group(P<0.05), and the expression levels of MALAT1 mRNA in the cells in siYAP-1 and siYAP-2 groups were significantly lower than those in Scramble siRNA group (P<0.05).Compared with pcDNA3.1-YAP group, the proliferation abilities of the A549 and H1299 cells in pcDNA3.1-YAP+siMALAT1-2 group were decreased(P<0.05),the migration ability of the H1299 cells in pcDNA3.1-YAP+siMALAT1-2 group was decreased(P<0.05). Conclusion: In the NSCLC cells, YAP promotes the cell proliferation and migration by regulating the expression of lncRNA MALAT1.

Key words: YAP, MALAT1, lung neoplasms, cell proliferation, migration

中图分类号: 

  • R734.2