Journal of Jilin University(Medicine Edition) ›› 2022, Vol. 48 ›› Issue (6): 1614-1622.doi: 10.13481/j.1671-587X.20220630
• Methodology • Previous Articles Next Articles
Tingyu RUAN1,Weidi SHI1,Xun MA1(),Jing WANG1(),Lichao KANG2,Dongdong DU2,Yuelan YIN3
Received:
2022-02-03
Online:
2022-11-28
Published:
2022-12-07
Contact:
Xun MA,Jing WANG
E-mail:maxun779@126.com;wjtry100@163.com
CLC Number:
Tingyu RUAN,Weidi SHI,Xun MA,Jing WANG,Lichao KANG,Dongdong DU,Yuelan YIN. Construction of NDRG1 interference vector and establishment of human brain microvascular endothelial cells stably over-expressing NDRG1[J].Journal of Jilin University(Medicine Edition), 2022, 48(6): 1614-1622.
Tab. 1
Target sequences of shRNA vector of NDRG1 gene and annealed DNA Oligo sequences"
Name | Target sequence | Annealed DNA Oligo sequence(5'-3') |
---|---|---|
shRNA- NDRG1-236 | GGAGCAGGACATCG-AGACTTT | F:CACCGGAGCAGGACATCGAGACTTTCAAGAGAAGTCTCGATGTCCT-GCTCCTTTTTTG |
R:GATCCAAAAAAGGAGCAGGACATCGAGACTTCTCTTGAAAGTCTCG-ATGTCCTGCTCC | ||
shRNA- NDRG1-242 | GGACATCGAGACT- TTACATGG | F:CACCGGACATCGAGACTTTACATGGTTCAAGAGACCATGTAAAGTC-TCGATGTCCTTTTTTG R:GATCCAAAAAAGGACATCGAGACTTTACATGGTCTCTTGAACCATG-TAAAGTCTCGATGTCC |
shRNA- NDRG1-251 | GACTTTACATGGC- TCTGTTCA | F:CACCGACTTTACATGGCTCTGTTCATTCAAGAGATGAACAGAGCCA-TGTAAAGTCTTTTTTG R:GATCCAAAAAAGACTTTACATGGCTCTGTTCATCTCTTGAATGAAC-AGAGCCATGTAAAGTC |
shRNA- NDRG1-613 | GCCTTGTCCTTAT- CAACGTGA | F:CACCGCCTTGTCCTTATCAACGTGATTCAAGAGATCACGTTGATAA-GGACAAGGCTTTTTTG R:GATCCAAAAAAGCCTTGTCCTTATCAACGTGATCTCTTGAATCACG-TTGATAAGGACAAGGC |
shRNA- NDRG1-239 | GCAGGACATCGAG- ACTTTACA | F:CACCGCAGGACATCGAGACTTTACATTCAAGAGATGTAAAGTCTCG-ATGTCCTGCTTTTTTG R:GATCCAAAAAAGCAGGACATCGAGACTTTACATCTCTTGAATGTAA-AGTCTCGATGTCCTGC |
shRNA- NDRG1-564 | GCCTACATCCTAA- CTCGATTT | F:CACCGCCTACATCCTAACTCGATTTCAAGAGAATCGAGTTAGGATGT-AGGCTTTTTTG R:GATCCAAAAAAGCCTACATCCTAACTCGATTCTCTTGAAATCGAGTT-AGGATGTAGGC |
shRNA- NDRG1-802 | GCAACCTGCACCT- GTTCATCA | F:CACCGCAACCTGCACCTGTTCATCATTCAAGAGATGATGAACAGGTG-CAGGTTGCTTTTTTG R:GATCCAAAAAAGCAACCTGCACCTGTTCATCATCTCTTGAATGATGA-ACAGGTGCAGGTTGC |
shRNA- NDRG1-1041 | GCTGAGGCCTTCA- AGTACTTC | F:CACCGCTGAGGCCTTCAAGTACTTCTTCAAGAGAGAAGTACTTGAAG-GCCTCAGCTTTTTTG R:GATCCAAAAAAGCTGAGGCCTTCAAGTACTTCTCTCTTGAAGAAGTA-CTTGAAGGCCTCAGC |
shRNA- NDRG1-NC | TTCTCCGAACGTG- TCACGT | F:CACCGTTCTCCGAACGTGTCACGTTTCAAGAGAACGTGACACGTTCGG-AGAATTTTTTG R:GATCCAAAAAATTCTCCGAACGTGTCACGTTCTCTTGAAACGTGACAC-GTTCGGAGAAC |
1 | 高 凡, 郑文亚, 刘 犇, 等. 单增李斯特菌穿越生理屏障机制的研究进展[J]. 中国人兽共患病学报, 2022, 38(1): 55-61. |
2 | MAURY M M, TSAI Y H, CHARLIER C, et al. Uncovering Listeria monocytogenes hypervirulence by harnessing its biodiversity[J]. Nat Genet, 2016, 48(3): 308-313. |
3 | 王 芳. N-myc下游调节基因1-NDRG1[J]. 昆明医科大学学报, 2014, 35(7): 1-4. |
4 | KITOWSKA A, PAWEŁCZYK T. N-myc downstream regulated 1 gene and its place in the cellular machinery[J]. Acta Biochim Pol, 2010, 57(1): 15-21. |
5 | BAE D H, JANSSON P J, HUANG M L, et al. The role of NDRG1 in the pathology and potential treatment of human cancers[J]. J Clin Pathol, 2013, 66(11): 911-917. |
6 | 徐冬冬, 王 韵, 章丽霞, 等. 小鼠宫颈癌组织放化疗前后NDRG1蛋白表达差异及预后意义[J]. 现代肿瘤医学, 2021, 29(20): 3522-3526. |
7 | 李成伟, 李圣青. N-myc下游调控基因1在呼吸系统疾病中的研究进展[J]. 复旦学报(医学版),2022,49(2): 282-288. |
8 | CAI K, EL-MERAHBI R, LOEFFLER M, et al. Ndrg1 promotes adipocyte differentiation and sustains their function[J]. Sci Rep, 2017, 7(1): 7191. |
9 | VILLODRE E S, GONG Y, HU X D, et al. NDRG1 expression is an independent prognostic factor in inflammatory breast cancer[J].Cancers, 2020,12(12): 3711. |
10 | 刘扬扬, 康立超, 马 勋, 等. 食源性单增李斯特菌LIPI-4基因的检测及序列分析[J]. 中国兽医学报, 2019, 39(12): 2380-2385. |
11 | ZHANG Y Y, DONG S L, CHEN H H, et al. Prevalence,genotypic characteristics and antibiotic resistance of Listeria monocytogenes from retail foods in bulk in Zhejiang Province, China[J]. Front Microbiol, 2019, 10: 1710. |
12 | CHEN Y T, CHEN M T, WANG J, et al. Heterogeneity, characteristics, and public health implications of Listeria monocytogenes in ready-to-eat foods and pasteurized milk in China[J]. Front Microbiol, 2020, 11: 642. |
13 | 刘扬扬, 康立超, 马 勋, 等. 食源性单核细胞增生李斯特菌毒力岛4携带株的分型、毒力及生物被膜形成能力的测定[J].中国预防兽医学报,2020,42(6):567-571. |
14 | 阮婷玉, 康立超, 刘扬扬, 等. 食源性单增李斯特菌毒力岛4基因缺失株的构建及其体外毒力研究[J/OL]. 中国动物传染病学报[2021-04-27].. |
15 | 李文艳, 陈滢滢, 董子怡, 等. 单增李斯特菌对小鼠滋养层细胞炎性体的激活[J]. 河北大学学报(自然科学版), 2022, 42(1): 73-77. |
16 | SALAMA P J, EMBAREK P, BAGARIA J, et al. Learning from listeria: safer food for all[J]. Lancet, 2018, 391(10137): 2305-2306. |
17 | LI W W, BAI L, FU P, et al. The epidemiology of Listeria monocytogenes in China[J]. Foodborne Pathog Dis, 2018, 15(8): 459-466. |
18 | LARKIN J, CHEN B S, SHI X H, et al. NDRG1 deficiency attenuates fetal growth and the intrauterine response to hypoxic injury[J]. Endocrinology, 2014, 155(3): 1099-1106. |
19 | SAHNI S, BAE D H, LANE D J R, et al. The metastasis suppressor, N-myc downstream-regulated gene 1 (NDRG1), inhibits stress-induced autophagy in cancer cells[J]. J Biol Chem, 2014, 289(14): 9692-9709. |
20 | 何宜静, 欧 琼, 李 婷, 等. 过表达NDRG1通过调控VEGF/sFlt-1轴增强缺氧条件下滋养细胞的促血管形成能力[J]. 安徽医科大学学报, 2020, 55(5): 745-750. |
21 | DAI X L, FU Y F, YE Y H. Increased NDRG1 expression suppresses angiogenesis via PI3K/AKT pathway in human placental cells[J]. Pregnancy Hypertens, 2020, 21: 106-110. |
22 | WATARI K, SHIBATA T, FUJITA H, et al. NDRG1 activates VEGF-A-induced angiogenesis through PLCγ1/ERK signaling in mouse vascular endothelial cells[J]. Commun Biol, 2020, 3(1): 107. |
23 | MERLOT A M, PORTER G M, SAHNI S, et al. The metastasis suppressor, NDRG1, differentially modulates the endoplasmic reticulum stress response[J]. Biochim Biophys Acta Mol Basis Dis, 2019, 1865(9): 2094-2110. |
24 | JIN W W, IBEAGHA-AWEMU E M, LIANG G X, et al. Transcriptome microRNA profiling of bovine mammary epithelial cells challenged with Escherichia coli or Staphylococcus aureus bacteria reveals pathogen directed microRNA expression profiles[J]. BMC Genomics, 2014, 15: 181. |
25 | LI X R, ZHANG P Y, WANG B B, et al. NDRG1 negatively regulates proliferation and Milk bio-synthesis of bovine epithelial cells via the mTOR signaling pathway[J]. Res Vet Sci, 2019, 124: 158-165. |
26 | 韩 帅, 陈 铭. 功能基因组学研究的技术方法[J]. 生命的化学, 2020, 40(3): 319-328. |
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