Journal of Jilin University Medicine Edition ›› 2015, Vol. 41 ›› Issue (06): 1134-1138.doi: 10.13481/j.1671-587x.20150607

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Biocompatibility and brain-targeting ability of nano-micelles on glioma cells

HAN Hailing1,2, MIAO Zhuang3, YUE Jun4, ZHAO Changfu2, JING Xiabin4, JIN Sunzi1, WANG Zhanfeng2,3   

  1. 1. Key Laboratory of Radiobiology, Ministry of Health School of Public Health, Jilin University, Changchun 130021, China;
    2. Affliatted Hospital, Beihua University, Jilin 132011, China;
    3. Department of Neurosurgery, China-Japan Union Hospital, Jilin University, Changchun 130033, China;
    4. State Key Lab of Polymer Physics and Chemistry, Changchun Institute of Applied Chemistry, Changchun 130022, China
  • Received:2015-05-05 Published:2016-01-11

Abstract:

Objective To investigate thebiocompatibility and safety of nanoscale brain-targeting-carrier micelles[poly(ethylene)-b-poly(lactic acid) /OX26 conjugate micells(copolymer/OX26)],and to explore its possibility as brain-targeted-drug carrier for brain glioma. Methods The C6 glioma cells were cultured in vitro and divided into experimental groups with different concentrations (10,20,40,80 mg·L-1) of nano micelle,and the medium without micelle was used as control group.The inhibitory effect of nano-micelles on the rat brain glioma C6 cells was examined by Trypan blue cell counting assay.Flow cytometry (FCM) was used to detect the changes of apoptosis and cell cycle of C6 cells,and confocal laser scanning microscope (CLSM) was performed to analyze the distribution of copolymer/OX26 into C6 cells. Results The results of Trypan blue cell counting assay showed copolymer/OX26 didn't affect the growth of C6 cells,and there were no significant differences in the number of C6 cells between control group and expreimental groups(P>0.05).The results of FCM showed that the cell cycle and and the apoptotic rates of C6 cells had no changes compared with control group(P>0.05).The results of CLSM showed that the fluorescence intensities in experimental groups were higher than those in blank micelles group and blank control group(P<0.05),and they were increased in dose-and time-dependent manner(P<0.05). Conclusion Copolymer/OX26 has no effect on the growth and apoptosis of glioma cells.By bonding OX26, copolymer/OX26 can significantly increase the intake of C6 cells on the nano micelles.

Key words: glioma, brain-targeting-carrier micelles, transferrin receptor, histocompatibility

CLC Number: 

  • R739.4