大鼠脂肪源性干细胞来源的施万样细胞的增殖能力

doi:10.13481/j.1671-587x.20170108

Abstract

Abstract:

Objective: To investigate the application and significance of Schwann-like cells in nerve regeneration field by detecting the proliferation ability of Schwann-like cells derived from rat adipose-derived stem cells(ADSCs).Methods: The ADSCs obtained from rat adipose tissue were isolated and cultured.The ADSCs at passage 4 were induced to differentiate into Schwann cells under the treatment of inducing factors,such as β-ME,ATRA,PDGF-AA,bFGF,Forskolin, and Heregulin-β. Immunofluorescence,Western blotting and Real-time PCR were performed to detect the expresson levels of the markers of Schwann cells(SCs) S100β,P75, and glial fibrillary acidic protein (GFAP).The proliferation ability of the Schwann like cells were analyzed using an MTT assay and the mitotic feature of Schwann like cells was also assessed.Results: After induction and differentiation,the Schwann-like cells were spindle and arranged as fence. S-100β,P75, and GFAP protein immunofluorescence were marked by Cy3/FITC,and the positive products were located in the cytoplasm and its processes.The Western blotting and Real-time PCR results showed that S-100β,P75, and GFAP protein and mRNA expression levels in the Schwann-like cells were obviously higher than those in ADSCs(P<0.01).Moreover,the Schwann-like cells possessed a strong proliferation ability of mitosis.Conclusion: ADSCs can be successfully induced to differentiate into Schwann-like cells and express the markers of SCs.In addition,the Schwann-like cells possess a strong proliferation ability of mitosis,revealing that they would be used as the seed cells for nerve tissue engineering.

Key words: adipose-derived stem cells, Schwann-like cells, cell proliferation, mitosis

CLC Number:

R322.8

FU Xiumei, YANG Zhenjiang, WANG Rongliang, WANG Jing, WANG Xiaojie, CHEN Zhihong, YANG Zhenjun. Proliferation ability of Schwann-like cells derived from rat adipose-derived stem cells and its significance[J].Journal of Jilin University Medicine Edition, 2017, 43(01): 36-41.

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Proliferation ability of Schwann-like cells derived from rat adipose-derived stem cells and its significance

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