Abstract:

Objective: To explore clinical significance of alpha 1 microglobulin (α1-MG) in the evaluation on liver function in the patients with chronic hepatitis B (CHB) through detecting the serum levels of α1-MG in the CHB patients with different liver function injuries.Methods: Total 243 CHB patients were divided into normal liver and renal function group(n=94),normal liver function and abnormal renal function group(n=20),abnormal liver function and normal renal function group(n=100),abnormal liver and renal function group(n=29),while 67 cases of healthy persons were selected as control group.The serum α1-MG levels of the subjects in various groups were measured by immune turbidimetry; the liver function indexes such as alanine aminotransferase (ALT),aspartate transaminase (AST), alkaline phosphatase (ALP),and the renal function indexes such as creatinine (CREA),blood urea nitrogen (BUN) were determinedby ultraviolet lactate dehydrogenase method,UV-malate dehydrogenase method,AMP buffer method,sarcosine oxidase method, and UV-glutamic acid dehydrogenase method.Results: Compared with control group,the level of α1-MG in serum of the patients in abnormal liver function and nomal renal function group was significantly decreased (P<0.05),the serum α1-MG levels of the patients in normal liver function and renal function group and abnormal liver and renal function group had no significant changes (P>0.05),and the serum α1-MG level of the patients in normal liver function and abnormal renal function group was significantly increased (P<0.01).According to α1-MG <10 mg·L^-1 as the standard for judging abnormal liver function,the positive rate of α1-MG in the patients in abnormal liver function and normal renal function group was 36% and the positive rate in abnormal liver and renal function group was 24%.As to α1-MG <15 mg·L^-1 as the standard,the positive rate of α1-MG in abnormal liver function and normal renal function group was 68%.The serum α1-MG level had negatively correlated with the ALT,AST and ALP of the patients in abnormal liver function and nomal renal function group (r=-0.934,r=-0.916,r=-0.847,P<0.01),and its values were decreased with the severity of liver injury.Conclusion: α1-MG detection has certain clinical significance in judging liver function in the patients with CHB,but it is necessary to exclude the interference caused by renal damage.

Key words: alpha 1-microglobulin, serological detection, liver function, renal function, hepatitis B