载VEGF/万古霉素的多层缓释微球对人胎盘源间充质干细胞增殖与成骨分化的影响

doi:10.13481/j.1671-587x.20170208

Abstract

Abstract: Objective: To investigate the influence of multilayer alginate chitosan sustained-release microspheres loading vascular endothelial growth factor(VEGF) and vancomycin in the proliferation and osteogenic differentiation of human plaacenta-derived mesen chymal stem cells(HPMSCs), and to provide theoretical basis for its clinical application in the repair of bone defect. Methods: The microspheres were prepared based on the previous research and HPMSCs were co-cultured with drug (VEGF/vancomycin)-loaded microspheres (drug-loaded microspheres+HPMSCs group), non-drug loaded microspheres (microspheres+HPMSCs group) and without any microspheres (HPMSCs group). Then the proliferation rate of HPMSCs was identified by CCK-8 kit. The osteogenic differentiation potential of HPMSCs was detected by Alizarin red staining and alkaline phosphatase (ALP) kit when the HPMSCs had been co-cultured with drug loaded microspheres in osteogenic medium (HPMSCs+ drug-loaded microspheres+induction group), non-drug loaded microspheres in osteogenic medium (HPMSCs+microspheres+induction group), without any microspheres in osteogenic medium (HPMSCs+induction group) and without any micropheres in normal medium (HPMSCs+PBS group) for 21 d. Results: Compared with HPMSCs group,the proliferation rates of HPMSCs in drug-loaded microspheres+HPMSCs and microspheres+HPMSCs groups had no significant changes (P>0.05).The calcium deposition in HPMSCs+drug-loaded microspheres+induction group was more than those in microspheress+HPMSCs+indution group and HPMSCs+induction group after Aalizarin red staining; the ALP activity in drug-loaded microspheres+HPMSCs+indution group was higher than those in microspheres+HPMSCs+indution group and HPMSCs+induction group (P<0.05),and the ALP activity in microspheres+HPMSCs+induction group was higher than that in HPMSCs+induction group(P<0.05). Conclusion: The sustained-release microspheres loading VEGF and vancomycin have no significant effect on the proliferation activity of HPMSCs and the microspheres could stimulate the osteogenic differentiation of HPMSCs.

Key words: vascular endothelial growth factor, alkalin phosphatase, vancomycin, stem cells, microsphere, osteogenesis, cell proliferation

CLC Number:

R318

LIU Kang, WANG Jinglong, YANG Junxing, SONG Lijie, XU Yichi, ZHAO Chuqiao, WANG Yao, LIU Zhihui, WANG Bowei. Influence of multilayersustained-release microspheres loading VEGF and vancomycin in proliferation and osteogenic differentiation of HPMSCs[J].Journal of Jilin University Medicine Edition, 2017, 43(02): 250-254.

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Influence of multilayersustained-release microspheres loading VEGF and vancomycin in proliferation and osteogenic differentiation of HPMSCs

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