Abstract:

To find the most effective way to obtain highly homogenous and undifferentiated human adipose-derived mesenchymal stem cells（hADSCs）,and induce the hADSCs to differentiate into adipocytes.Methods hADSCs were isolated from human adipose tissue by collagenase digestion and adherence to flasks.hADSCs were differentiated into adipocytes by using dexamethasone,indomethacin,IBMX and insulin.Results hADSCs had unique immunophenotypes and they were positive for CD73,CD44,CD166,CD105 and CD29,but negative for CD31,CD34,CD45 and HLA-DR.hADSCs had the typical proliferative characteristics of stem cells，most of them were in the resting phase ，only a few cells were in the active proliferative phase.They could be induced to differentiate into adipocytes.Conclusion  It is successful to isolate and obtain highly homologous hADSCs,and it was  also successful  in inducing the hADSCs to differentiate into adipocytes in vitro.

Key words: stem cells, flow cytometry, adipocytes