Abstract:

Abstract:Objective To study the influence of 3′-azido-3′-deoxythymidine (AZT) on the apoptosis of  gastric
cancer cell line SCG 7901 and the expressions of BAX protein and proliferation cell nuclear antigen (PCNA)  and explore themechanisms.Methods The gastric cancer cell line SCG 7901 treated with 1.0 mmol?L-1 AZT for 6 d were used as AZT group,at the same time,control group was set up.The apoptotic rates  of SGC 7901 in two groups weredetected with MG-P-Mstaining. The expressions of Bax protein and PCNA  of  gastric cancer cell line SCG 7901 6 d after drug administrationwere determined by  immunohistochemical technique(EnVision). Results The apoptotic rates in AZT proup and control group were(16.421±0.713)% and　(3.366±0.271)% respectively, the  apoptotic rate  in AZT group was  significantly higher thanthat in  control group(P<0.05). Compared with control group,the expression of Bax protein was significantly increased (16.851%±10.064% vs 47.389%±18.042%,P<0.05)　 and the expression of PCNA  was significantly decreased(23.446%±13.055% vs62.332±22.767%，P<0.05) in AZT group. Conclusion AZT can induce  the apoptosis of gastric cancer cell line SCG7901,promote the expression of Bax protein and inhibit the expression of PCNA.

Key words: apoptosis, Bax, proliferation cell nuclear antigen, 3′-azido-3′-deoxythymidin;gastric cancer cell line SCG7901