Abstract:

Abstract:Objective
To investigate the effect of rhein lysinate (RHL) on the proliferation and apoptosis of human lung cancer cell line H460 and the role of human epidermal growth factor receptor-2 (HER2) signal pathway in apoptosis,and to provide theoretical foundation for clinical research on RHL against lung cancer. Methods The H460 cells in which HER2 was over-expressed in logarithm growth phase were selected and randomly divided into blank control group and different concentrations (25,50,75,100,125,and 150 μmol•L^-1) of RHL groups. MTT assay was used to detect the proliferation abilities of H460 cells in various groups. The H460 cells were randomly divided into blank control group and 50,100,and 150 μmol•L^-1 RHL groups; flow cytometry was used to analyze apoptosis in various groups after 48 h. The expression levels of  apoptosis-related proteins and   HER2 signal pathway proteins were detected after 48 h by Western blotting method. Results  After 48 h cell culture,the cell viability rates in RHL groups were lower than that in blank control group (P<0.05),whereas the apoptotic rates in RHL groups were higher than that in blank control group (P<0.05). The inhibition of cell proliferation of RHL presented a dose-dependent manner in H460 cells,and so the apoptosis induced by RHL. The expressions of cleaved poly ADP-ribose polymerase (PARP) and caspase-3/7 in RHL groups were higher than those in blank control group (P<0.05) after 48 h cell culture in H460 cells,whereas the expressions of phosphorylation of HER2 and AKT and NF-κB were lower than those in control group (P<0.05).Conclusion RHL can induce apoptosis by inhibiting HER2/AKT/NF-κB signal pathway in H460 cells.

Key words: rhein lysinate, lung neoplasms, apoptosis, HER2 signal pathway