Journal of Jilin University Medicine Edition ›› 2015, Vol. 41 ›› Issue (02): 383-388.doi: 10.13481/j.1671-587x.20150235

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Association between single nucleotide polymorphisms in 3'UTR of ERAP1 gene and essential hypertension in Jilin Han population

YANG Sibao, LIU Xueyan, LI Bing, DING Mei, HE Yuquan, YANG Ping   

  1. Department of Cardiology, China-Japan Union Hospital, Jilin University, Changchun 130033, China
  • Received:2014-09-25 Online:2015-03-28 Published:2015-04-04

Abstract:

Objective To explore the expression levels of ERAP1 gene in the peripheral blood of the essential hypertension patients, and to identify the single nucleotide polymorphisms(SNPs) in 3'UTR of ERAP1 gene associated with essential hypertension.Methods 300 patients with hypertension (hypertension group) and 233 normal controls (control group) were recruited to conduct a case-control study in Jilin Han population. The expression levels of ERAP1 in the peripheral blood of the objects were detected by ELISA method;the SNPs in 3' UTR of ERAP1 gene were identified by pool-sequencing method;the genotyping was performed for each participant by PCR sequencing method.Results The expression levels of ERAP1 in the peripheral blood of the patients in hypertension group were significantly lower than those in control group (P<0.05). Two SNPs,E20-790G >A and E20-816C >T,were identified in 3'UTR of ERAP1 gene. The distribution of the genotypic and allelic frequencies of E20-816C >T polymorphism was significantly different between hypertension group and control group (P<0.05). The Logistic regression results showed that adjusting for the confounding factors,the genotypic frequency of CT gene in hypertension group was decreased significantly(P<0.05,OR=0.145,95% CI:0.031-0.675).Conclusion E20-816C>T polymorphism may be related to essential hypertension in the Jilin Han population,and the decreasing genotypic frequency of CT gene may be related to the down-regulated expression of ERAP1.

Key words: essential hypertension, endoplasmic reticulum aminopeptidase 1, single nucleotide polymorphism, molecular genetics, association study

CLC Number: 

  • R544.11