力达霉素抑制K562细胞增殖和诱导凋亡作用及其机制

doi:10.13481/j.1671-587x.20160310

Abstract

Abstract:

Objective: To explore the inhibitory effect of lidamycin (LDM) on the cellproliferation and apoptosis induction effect in the human chronic myeloid leukemia (CML) K562 cells,and to discuss the mechanisms. Methods: The K562 cells at logarithmic growth phase were selected and treated with different concentrations (0.01,0.10 and 1.00 nmol·L^-1) of LDM for 48 h and they were used as 0.01,0.10,and 1.00 nmol·L^-1 LDM groups,and the normal cells without treatment were regarded as control group.Trypan blue staining was used to detect the inhibitory rates of growth of the K562 cells in various groups;the cell viability was determined by MTT assay;the apoptotic morphology of cells was investigated by acridine orange/ethidium bromide (AO/EB) staining;the apoptotic rate of cells was detected by Annexin Ⅴ-FITC/PI double staining and flow cytometry;the relative expression levels of caspase-3 and caspase-8 in K562 cells were measured by Western blotting analysis. Results: The Trypan blue staining results showed that LDM could inhibit the proliferation of K562 cells and the inhibitory rates of growth of the K562 cells in different concentrations of LDM groups were higher than that in control group (P<0.05).The MTT results showed that the cell viability was decreased with the increasing of LDM concentration and the 50% inhibitory concentration (IC₅₀) value was (0.1 ± 3.2) nmol·L^-1.The AO/EB double staining results showed that LDM could induce the apoptosis of K562 cells under fluorescence microscope (the cytoplasm was in buds and the nucleus were broken into points).The flow cytometry results showed that the apoptotic rates of cells in different concentrations of LDM groups were higher than that in control group (P<0.05).The Western blotting analysis results showed that the relative expression levels of caspase-8 and caspase-3 in the K562 cells in 0.10 nmol·L^-1 and 1.00 nmol·L^-1 LDM groups were higher than those in control group (P<0.01);the relative expression levels of caspase-8 and caspase-3 in the K562 cells in 0.01 nmol·L^-1 LDM group had no significant differences compared with control group(P＞0.05). Conclusion: LDM could inhibit the proliferation of K562 cells.Moreover,low concentration of LDM may induce the apoptosis by up-regulating the expressions of caspase-8 and caspase-3.

Key words: lidamycin, K562 cells, cell proliferation, apoptosis, caspase-8, caspase-3

CLC Number:

R329.28

CHEN Jing, YAN Zhenyu, LIU Zhiyong, ZHAO Jie, HE Baoling. Inhibitory effect of lidamycin on cell proliferation and induction of apoptosis in human leukemia K562 cells and their mechanisms[J].Journal of Jilin University Medicine Edition, 2016, 42(03): 467-472.

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Inhibitory effect of lidamycin on cell proliferation and induction of apoptosis in human leukemia K562 cells and their mechanisms

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