“调脏通络”电针对糖尿病周围神经病变大鼠坐骨神经细胞中GRP78表达的影响及其神经保护作用

doi:10.13481/j.1671-587x.20180603

Abstract

Abstract: Objective: To investigate the expression of glucose regulating protein 78(GRP78) in the sciatic nerve cells of the rats with diabetic peripheral neuropathy (DPN), and to elucidate the clinical significance and mechanism of Adjust Zang Dredge Meridianelectroacupuncture in the treatment of DPN.Methods: A total of 86 adult male SD rats were divided into normal control group(n=5)and model group (n=81). The diabetic rat models were built through one-time injection of streptozocin(STZ), and then the diabetic rats were randomly divided into model control group(n=27),medicine intervention group(n=26), and electroacupuncture intervention group(n=26).The rats in normal control group and model control group were fed with the maintenance feed.During the course of experiment, no special treatment was done for the rats in normal control group and model control group.The rats in drug intervention group were treated with intragastric administration of mecobalamin,20 mg·kg^-1·d^-1,once per day. The rats in electroacupuncture intervention group were treated with Adjust Zang Dredge Meridian electroacupuncture in the bilateral Feishu, Pishu, Shenshu, Hegu, Zusanli,Sanyinjiao and Taichong, once per day, 30 min per time.The thermal sensitivity value, motor nerve conduction velocity(MCV)and sensory nerve conduction velocity(SCV)of the tibial nerve of the rats in various groups were measured, the ultrastructures of sciatic nerve of the rats in various groups were observed under transmission electron microscope, the apoptotic rates of sciatic nerve cells of the rats in various groups were detected by TUNEL method, the expressions of GRP78 in sciatic nerve cells of the rats in various groups were detected by immumofluorescence,and the expression levels of GRP78 in sciatic nerve of the rats in various groups were detected by Western blotting method.Results: Compared with medicine intervention group, the thermal sensitivity value of the rats in electroacupuncture intervention group was significantly decresed (P<0.01). Compared with model control group, the MCV and SCV of tibial nerve of the rats in medicine intervention group and electroacupuncture intervention group were increased after 12-week-intervention (P<0.01); compared with before intervention, the MCV and SCV of tibial nerve of the rats in various groups were decreased after 12-week-intervention (P<0.01).Under transmission electron microscope, some of the myelin sheath lamellae of sciatic nerve of the rats in model control group showed irregular membrane-like mass, and most of the thicker myelinated nerve fibers were seriously involved; the above changes were alleviated in medicine intervention group and electroacupuncture intervention group.The TUNEL assay results showed that compared with model control group, the apoptotic rates of sciatic nerve cells of the rats in medicine intervention group and electroacupuncture intervention group were significantly decreased (P<0.01). The Western blotting results showed that compared with model control group,the expression levels of GRP78 in sciatic nerve cells of the rats in medicine intervention group and electroacupuncture intervention group were significantly decreased (P<0.05).Conclusion: Adjust Zang Dredge Meridian electroacupuncture can reduce the expression level of GRP78 in sciatic nerve cells of the DPN rats, inhibit the occurrence of endoplasmic reticulum stress(ERS), decrease the apoptotic rate of cells, and protect the nerves.

Key words: diabetic peripheral neuropathy, Adjust Zang Dredge Meridian electroacupuncture, endoplasmic reticulum stress, glucose regulated protein 78

CLC Number:

R587.1

PAN Hong, WANG Yufeng, HUANG Haipeng, ZHANG Liying, ZHONG Zhen, MA Shiqi, DONG Li, WANG Hongfeng. Influence of Adjust Zang Dredge Meridian electroacupuncture in expression of GRP78 in sciatic nerve cells of rats with diabetic peripheral neuropathy and its neuroprotective effect[J].Journal of Jilin University(Medicine Edition), 2018, 44(06): 1124-1130.

References

[1] Boulton AJ, Vinik AI, Arezzo JC, et al. Diabetic neuropathies:a statement by the American Diabetes Association[J]. Diabetes Care, 2005, 28(4):956-962.
[2] Hamid HS, Mervak CM, Münch AE, et al. Hyperglycemia-and neuropathy-induced changes in mitochondria within sensory nerves[J]. Ann Clin Transl Neurol,2014, 1(10):799-812.
[3] Edwards JL, Vincent AM, Cheng HT, et al. Diabetic neuropathy:mechanisms to management[J]. Pharmacol Ther, 2008, 120(1):1-34.
[4] Sandireddy R, Yerra VG, Areti A, et al. Neuroinflammation and oxidative stress in diabetic neuropathy:futuristic strategies based on these targets[J]. Int J Endocrinol, 2014, 2014(1):674987.
[5] Tuttolomondo A, Maida C, Pinto A. Diabetic foot syndrome:Immune-inflammatory features as possible cardiovascular markers in diabetes[J]. WorldJ Orthop, 2015, 6(1):62-76.
[6] Lane JT. The role of retinoids in the induction of nerve growth factor:a potential treatment for diabetic neuropathy[J]. Transl Res,2014, 164(3):193-195.
[7] Lupachyk S, Watcho P, Stavnllchuk R, et al. Endoplasmic reticulum stress plays a key role in the pathogenesis of diabetic peripheral neuropathy[J]. Diabetes, 2013, 62(3):944-952.
[8] 杨欢,郑小兰,徐国海,等.电针治疗糖尿病神经病理性疼痛机制的研究进展[J].北京:中国疼痛医学杂志,2015,21(11):855-858.
[9] 郭义,方剑乔.实验针灸学[M]. 北京:中国中医药出版社, 2012:46-49.
[10] Yangihashi S,Kamijo M,Watanabe K.Reduced mychanted fiber size correlates with loss of axonal neurofilaments in peripheral nerve of chronically streptozotocin diabetic rats[J].Am J Pathol,1990,136(6):1363-1373.
[11] Paschen W, Mengesdorf T. Endoplasmic reticulum stress response and neurodegeneration[J]. Cell Calcium, 2005, 38(3/4):409-415.
[12] Liu H,Qian J,Wang F.Expression of two endoplasmic reticulum stress markers,GRP78 and GADD153,in rats retinal detachment model and its implication[J].Eye(Lond),2010,24(1):137.
[13] Lupachyk S, Watcho P, Stavniichuk R, et al. Endoplasmic reticulum stress plays a key role in the pathogenesis of diabetic peripheral neuropathy[J]. Diabetes, 2013,62(3):944-952.
[14] Yang GH, Li S, Pestka JJ. Down-regulation of the endoplasmic reticulum chaperone GRP78/BiP by vomitoxin (Deoxynivalenol)[J]. Toxicol Appl Pharmacol, 2000, 162(3):207-217.
[15] Hayashi T, Saito A, Okuno S, et al. Induction of GRP78 by ischemic preconditioning reduces endoplasmic reticulum stress and prevents delayed neuronal cell death[J]. J Cereb Blood Flow Metab, 2003, 23(8):949-961.
[16] Oida Y, Izuta H, Oyagi A, et al. Induction of BiP, an ER-resident protein, prevents the neuronal death induced by transient forebrain ischemia in gerbil[J]. Brain Res, 2008, 1208:217-224.
[17] Reddy RK,Mao C,Baumeister P,et al.Endoplasmic reticulum chaperone protein GRP78 protects cells from apoptosis induced by topoisomerase inhibitors:role of ATP binding site in suppression of caspase-7 activation[J]. Biol Chem,2003,278(23):20915-20924.
[18] Ermakova SP, Kang BS, Choi BY, et al. (-)-Epigallocatechin gallate overcomes resistance to etoposide-induced cell death by targeting the molecular chaperone glucose-regulated protein 78[J]. Cancer Res, 2006,66(18):9260-9269.
[19] Ranganathan AC, Zhang L, Adam AP, et al. Functional coupling of p38-induced up-regulation of BiP and activation of RNA-dependent protein kinase-like endoplasmic reticulum kinase to drug resistance of dormant carcinoma cells[J]. Cancer Res, 2006, 66(3):1702-1711.
[20] 陈晓蕾,贾农,柯亭羽,等.2型糖尿病合并亚临床甲状腺功能减低与肾脏损害相关分析[J].中国实用内科杂志,2018,38(1):65-68.

Influence of Adjust Zang Dredge Meridian electroacupuncture in expression of GRP78 in sciatic nerve cells of rats with diabetic peripheral neuropathy and its neuroprotective effect

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