菩人丹超微粉对糖尿病大鼠视网膜血流动力学和细胞因子表达的影响

doi:10.13481/j.1671-587x.20190111

Abstract

Abstract: Objective: To investigate the effects of Purendan Superfine Powder(PRD) on the retinal hemodynamics and the expressions of cytokines in the diabetic rats, and to elucidate its protective effect on diabetic retinopathy(DR).Methods: Thirty-six male SD rats were randomly divided into control group, diabetes group, and PRD group(n=12). The diabetes rat models were established by intraperitoneal injecting streptozotocin(STZ) in diabetes group and PRD group. After successful modeling, the diabetic rats in PRD group were given PRD (1.8 g ·kg^-1) by gavage for 3 months. The hemodynamics parameters of central retinal artery(CRA) of the rats were measured with color Doppler ultrasound. The expression levels of vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF) mRNA in retina tissue were detected by reverse transcription polymerase chain reaction (RT-PCR). Immunohistochemical staining and Western blotting methods were used to detect the expression levels of VEGF and bFGF proteins in retina tissue of the rats.Results: Compared with control group, the blood glucose, urine volume, liver index and kidney index of the rats in diabetes group were significantly increased(P<0.05), the peak systolic velocity(PSV), end diastolic velocity(EDV) and mean velocity(MV) of CRA were significantly decreased(P<0.05), the resistant index(RI) and pulsatility index(PI) of CRA were significantly increased(P<0.05), and the expression levels of VEGF and bFGF mRNA and protein in retina tissue were significantly increased(P<0.05). Compared with diabetes group, the blood glucose, urine volume, liver index and kidney index of the rats in PRD group were significantly decreased(P<0.05), the PSV, EDV and MV of CRA were increased(P<0.05), the RI and PI of CRA were decreased(P<0.05), the expression levels of VEGF and bFGF mRNA and protein in retina tissue were significantly reduced(P<0.05).Conclusion: PRD can protect the diabetic retinal microvascular injury by reducing the blood glucose level, increasing the blood supply of retinal tissue, increasing the amount of microcirculation blood perfusion, reducing the vascular bed perfusion resistance, and reducing the expression of VEGF and bFGF.

Key words: diabetes mellitus,experiment, diabetic retinopathy, Purendan Superfine Powder, hemodynamics, vascular endothelial growth factor, basic fibroblast growth factor

CLC Number:

R285.5

WANG Haibing, DONG Zhijun, GUO Litao, SHI Jing, DONG Weili. Effects of Purendan Superfine Powder on retinal hemodynamics and expressions of cytokines in diabetic rats[J].Journal of Jilin University(Medicine Edition), 2019, 45(01): 57-62.

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Effects of Purendan Superfine Powder on retinal hemodynamics and expressions of cytokines in diabetic rats

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