Journal of Jilin University(Medicine Edition) ›› 2021, Vol. 47 ›› Issue (2): 390-396.doi: 10.13481/j.1671-587X.20210219

• Research in basic medicine • Previous Articles     Next Articles

Therapeutic effect of total glucosides of paeony in model rats with rheumatoid arthritis by mediating TLR4/NF-κB signaling pathway and its mechanisim

Lijun YAN,Shengquan TONG(),Jing LIU,Dongmei GAO,Nanfang CHEN,Jie HU   

  1. Department of Rheumatology Immunology,Tangshan Workers’ Hospital,Hebei Medical University,Hebei 063000,China
  • Received:2020-08-30 Online:2021-03-28 Published:2021-03-25
  • Contact: Shengquan TONG E-mail:lijunyan84@163.com

Abstract: Objective

To investigate the effects of total glucosides of paeony (TGP) on joint condition, inflammatory factors and apoptosis factors in the rheumatoid arthritis (RA) rats by regulating the Toll-like receptor 4(TLR4) /nuclear factor-κB(NF-κB) signaling pathway, and to clarify its mechanism.

Methods

The collagen-induced arthritis (CIA) rat models were established with bovine type Ⅱ collagen and complete Freund’s adjuvant, and the CIA model rats were randomly divided into CIA group, low dose (25 mg·kg-1)of TGP(TGP-LD) group, middle dose (50 mg·kg-1) of TGP(TGP-MD) group, and high dose (100 mg·kg-1) of TGP(TGP-HD), with 10 rats in each group.Another 10 healthy SD rats were selected as control group . The body weights, arthritis indexes and joint swelling degrees of the rats in each group were measured, and the levels of tumor necrosis factor-α (TNF-α),interleukin-6 (IL-6) and interleukin-1β (IL-1β) in serum of the rats in each group were detected by ELISA method. The mRNA and protein expression levels of TLR4, NF-κB, Cleaved caspase-3, B cell lymphoma -2 (Bcl-2) and Bcl-2 associated X protein (Bax) in joint synovium tissue of the rats in each group were detected by Real-time fluorescence quantitative PCR(RT-qPCR) and Western blotting methods.

Results

Compared with control group, the body weight and the expression levels of Cleaved caspase-3 and Bax mRNA and proteins in joint synovium tissue of the rats in CIA group were significantly decreased (P<0.05), while the arthritis index, the joint swelling degree, the expression levels of Bcl-2, TLR4,and NF-κB p65 mRNA and proteins in joint synovium tissue, and the TNF-α, IL-6, and IL-1β levels in serum of the rats in CIA group were significantly increased (P<0.05). Compared with CIA group,the body weights and the expression levels of Cleaved caspase-3 and Bax mRNA and protein in joint synovium tissue of the rats in different doses of TGP groups were significantly increased(P<0.05), while the arthritis indexes,the joint swelling degrees, the expression levels of Bcl-2, TLR4,and NF-κB p65 mRNA and proteins in joint synovium tissue, and the TNF-α, IL-6, IL-1β levels in serum of the rats were significantly decreased(P<0.05).Compared with TGP-LD group,the body weights and the expression levels of Cleaved caspase-3 and Bax mRNA and proteins in joint synovium tissue of the rats in TGP-MD group and TGP-HD group were significantly increased(P<0.05),while the arthritis indexes, the joint swelling degrees, the expression levels of TLR4,NF-κB,p65 and Bcl-2 in joint synovium tissue, and the TNF-α, IL-6, and IL-1β levels in serum of the rats were significantly decreased(P<0.05).

Conclusion

TGP can improve the symptoms of CIA, reduce the inflammatory reaction and promote the apoptosis of synovial tissue by inhibiting TLR4/NF- κB signal pathway.

Key words: total glucoside of paeony, Toll-like receptor 4, nuclear factor-κB, rheumatoid arthritis, inflammation, synovial

CLC Number: 

  • R684.3