Journal of Jilin University(Medicine Edition) ›› 2021, Vol. 47 ›› Issue (2): 390-396.doi: 10.13481/j.1671-587X.20210219
• Research in basic medicine • Previous Articles Next Articles
Lijun YAN,Shengquan TONG(),Jing LIU,Dongmei GAO,Nanfang CHEN,Jie HU
Received:
2020-08-30
Online:
2021-03-28
Published:
2021-03-25
Contact:
Shengquan TONG
E-mail:lijunyan84@163.com
CLC Number:
Lijun YAN,Shengquan TONG,Jing LIU,Dongmei GAO,Nanfang CHEN,Jie HU. Therapeutic effect of total glucosides of paeony in model rats with rheumatoid arthritis by mediating TLR4/NF-κB signaling pathway and its mechanisim[J].Journal of Jilin University(Medicine Edition), 2021, 47(2): 390-396.
Tab. 1
Primer sequences of PCR"
Primer | Sequence (5'-3') |
---|---|
NF-κB p65 | Forward:ACGATCTGTTTCCCCTCATC |
Reserse: TGCTTCTCTCCCCAGGAATA | |
TLR4 | Forward:TTGCCTTCATTACAGGGACTT |
Reserse: AGATACCGGTGGAGGCTGACT | |
Cleaved caspase-3 | Forward: AGATGGTTTGAGCCTGAGCA |
Reserse: CAGTGCGTATGGAGAAATGG | |
Bax | Forward: GAGACACCTGAGCTGACCTTG |
Reserse: GCTCCATGTTGTTGTCCAGTT | |
Bcl-2 | Forward: GGTGAACTGGGGGAGGATTG |
Reserse: AGAGCGATGTTGTCCACCAG | |
β-actin | Forward:GCCATGTACGTAGCCATCCA |
Reserse: GAACCGCTCATTGCCGATAG |
Tab. 1
Levels of TNF-α, IL-6 and IL-1β in serum of rats in various groups [n=10,x±s,ρB/(ng·L-1)]"
Group | TNF-α | IL-6 | IL-1β |
---|---|---|---|
Control | 49.31±4.82 | 28.64±3.06 | 21.17±1.29 |
CIA | 107.63±16.29* | 83.43±10.02* | 68.35±9.63* |
TGP-LD | 79.26±8.20△ | 64.57±7.79△ | 57.68±6.26△ |
TGP-MD | 64.11±7.13△# | 60.32±7.25△# | 42.44±5.03△# |
TGP-HD | 58.20±5.61△# | 42.64±5.87△# | 37.29±2.51△# |
Tab. 2
Expression levels of Cleaved caspase-3,Bax and Bcl-2 mRNA in joint synovial tissue of rats in various groups"
Group | Cleaved caspase-3 | Bax | Bcl-2 |
---|---|---|---|
Control | 1.00±0.02 | 0.99±0.04 | 1.01±0.03 |
CIA | 0.41±0.05* | 0.35±0.05* | 2.95±0.24* |
TGP-LD | 0.56±0.07△ | 0.54±0.07△ | 2.46±0.17△ |
TGP-MD | 0.75±0.04△# | 0.63±0.05△# | 2.07±0.15△# |
TGP-HD | 0.86±0.06△# | 0.82±0.09△# | 1.69±0.18△# |
Tab. 3
Expression levels of Cleaved caspase-3, Bax and Bcl-2 proteins in joint synovium tissue of rats in various groups"
Group | Cleaved caspase-3 | Bax | Bcl-2 |
---|---|---|---|
Control | 0.63±0.05 | 0.66±0.08 | 0.82±0.07 |
CIA | 0.38±0.04* | 0.26±0.04* | 1.98±0.32* |
TGP-LD | 0.47±0.06△ | 0.41±0.02△ | 1.67±0.21△ |
TGP-MD | 0.60±0.09△# | 0.47±0.04△# | 1.34±0.16△# |
TGP-HD | 0.69±0.12△# | 0.56±0.07△# | 0.97±0.12△# |
Tab. 4
Expression levels of TLR4,NF-κB p65 mRNA and protein in joint synovium tissue of rats in various groups"
Group | TLR4 mRNA | NF-κB p65 mRNA | TLR4 protein | NF-κB p65 protein |
---|---|---|---|---|
Control | 1.01±0.03 | 1.00±0.05 | 0.52±0.08 | 0.31±0.04 |
CIA | 3.65±0.38* | 2.78±0.21* | 1.02±0.11* | 0.78±0.06* |
TGP-LD | 3.18±0.29△ | 2.25±0.23△ | 0.89±0.08△ | 0.63±0.05△ |
TGP-MD | 2.74±0.20△# | 1.98±0.17△# | 0.77±0.05△# | 0.55±0.05△# |
TGP-HD | 1.73±0.15△# | 1.36±0.11△# | 0.48±0.04△# | 0.35±0.04△# |
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