Journal of Jilin University(Medicine Edition) ›› 2021, Vol. 47 ›› Issue (2): 453-459.doi: 10.13481/j.1671-587X.20210226
• Research in clinical medicine • Previous Articles Next Articles
Received:
2020-07-17
Online:
2021-03-28
Published:
2021-03-25
Contact:
Yongchen LYU
E-mail:15543430749@126.com
CLC Number:
Weili HUANG,Yongchen LYU. Expressions of miR-1915-3p and Bcl-2 in human colon cancer and their clinical significances[J].Journal of Jilin University(Medicine Edition), 2021, 47(2): 453-459.
Tab.1
Primer sequences used in experiment"
Primer | Sequence (5'-3') |
---|---|
miR-1915-3p-RT | GCACTTCAGTGTCGTGGTCAGTGACGGC- AATTGAAGTGCCCCGCCGC |
miR-1915-3p | F:CAGACGACCATCAGCCCCAGGGCGA |
R:CGCGGATCCATGTCTATTATTACAG | |
U6 | F:GCTTCGGCAGCACATATACTAAAAT |
R:CGCTTCACGAATTTGCGTGTCAT | |
Bcl-2 | F:AGTGGGATGCGGGAGATGT |
R:CGGGCTGGGAGGAGAAGA | |
GAPDH | F:CATTCAAGACCGGACAGAGG |
R:ACATACTCAGCACCAGCATCACC |
Tab.2
The relationships between the expression levels of miR-1915-3p and Bcl-2 and clinicopathological features in patients with colon cancer"
Clinicopathological feature | n | miR-1915-3p | χ2 | P | Bcl-2 | χ2 | P | ||
---|---|---|---|---|---|---|---|---|---|
Low | High | Low | High | ||||||
Gender | 0.001 | 0.972 | 0.029 | 0.864 | |||||
Male | 26 | 17 | 9 | 9 | 17 | ||||
Female | 14 | 10 | 4 | 6 | 8 | ||||
Age (year) | 1.819 | 0.177 | 0.006 | 0.936 | |||||
<60 | 23 | 18 | 5 | 4 | 19 | ||||
≥60 | 17 | 9 | 8 | 4 | 13 | ||||
Tumor size(l/cm) | 0.459 | 0.498 | 0.001 | 0.974 | |||||
<5 | 12 | 7 | 5 | 6 | 6 | ||||
≥5 | 28 | 21 | 7 | 9 | 12 | ||||
Degree of differentiation | 6.567 | 0.038 | 0.688 | 0.709 | |||||
Low differentiation | 11 | 11 | 0 | 4 | 7 | ||||
Moderate differentiation | 16 | 14 | 2 | 5 | 11 | ||||
High differentiation | 13 | 8 | 5 | 6 | 7 | ||||
Lymph node metastasis | 5.260 | 0.022 | 3.472 | 0.062 | |||||
Yes | 24 | 23 | 1 | 3 | 21 | ||||
No | 16 | 10 | 6 | 7 | 9 | ||||
TNM stage | 1.284 | 0.257 | 4.167 | 0.041 | |||||
Ⅰ-Ⅱ | 15 | 8 | 7 | 6 | 9 | ||||
Ⅲ-Ⅳ | 25 | 19 | 6 | 2 | 23 |
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