TRIM24蛋白在人肾透明细胞癌组织中的表达及其临床意义

doi:10.13481/j.1671-587X.20250223

Abstract

Abstract:

Objective To discuss the expression of tripartite motif-containing protein 24 （TRIM24） in the clear cell renal cell carcinoma （ccRCC） tissue， and to clarify its relationships with the clinicopathological features and prognosis of the ccRCC patients. Methods The cancer and paracancer normal tissues were collected from 90 ccRCC patients who had not undergone preoperative radiotherapy or chemotherapy. Tissue microarray and immunohistochemistry were used to detect the expression levels of TRIM24 protein in ccRCC tissue. The differences in TRIM24 protein expression between ccRCC and paracancer normal tissues were analyzed. The score of TRIM24 protein expression and the average value were calculated， and based on the average value， the patients were classified into TRIM24 protein low-expression and TRIM24 protein high-expression groups. The associations between the TRIM24 protein expression and different clinicopathological features of the patients were analyzed， and the relationship between the TRIM24 protein expression and the prognosis of the patients was analyzed. Results The immunohistochemistry results showed that the TRIM24 protein was expressed in both the nucleus and cytoplasm of the ccRCC tumor cells， and there were significant differences in the TRIM24 protein expression level in ccRCC tissue when compared with paracancer normal tissue （P<0.05）. The TRIM24 protein expression in the nucleus of ccRCC tissue was associated with the patient’s age， gender， and tumor size （P<0.05）. The Kaplan-Meier survival analysis results showed that the overall survivals of the patients with high TRIM24 protein expression in the cytoplasm of ccRCC tissue， older age， and higher pathological grade were shorter than those with low TRIM24 protein expression， younger age， and lower pathological grade （P<0.05）. The multivariate Cox regression analysis results showed that the prognosis of the patients with high TRIM24 protein expression in the cytoplasm and higher pathological grade were poorer compared with the patients with low TRIM24 protein expression and lower pathological grade （P<0.05）. Conclusion The ccRCC patients with high TRIM24 expression in the cytoplasm of ccRCC tumor tissue and higher pathological grade have the lower postoperative survival rates and poorer prognosis.

Key words: Clear cell renal cell carcinoma, Triple motif protein 24, Immunohistochemistry, Pathological grade, Prognosis

CLC Number:

R737.11

Haiying GENG,Yan YU,Chunmei DAI,Youfeng WEN,Ning LI. Expression of TRIM24 protein in human clear cell renal cell carcinoma tissue and its clinical significance[J].Journal of Jilin University(Medicine Edition), 2025, 51(2): 486-492.

Figures/Tables 5

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References 25

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Parameter	n	Expression of TRIM24（Cytoplasm）		χ²	P	Expression of TRIM24（Nucleus）		χ²	P
Parameter	n	Low	High	χ²	P	Low	High	χ²	P
Age (year)
<60	41	23	18	0.294	0.587	16	25	4.054	0.044
≥60	38	19	19	0.294	0.587	7	31	4.054	0.044
Sex
Female	31	17	14	0.057	0.811	5	26	4.168	0.041
Male	48	25	23	0.057	0.811	18	30	4.168	0.041
Grade
Ⅰ/Ⅱ	68	39	29	3.441	0.064	21	47	0.253	0.615^a
Ⅲ/Ⅳ	11	3	8	3.441	0.064	2	9	0.253	0.615^a
T stage
T1/T2	75	41	34	0.415	0.519^a	23	52	-	0.316^b
T3	4	1	3	0.415	0.519^a	0	4	-	0.316^b
N stage
N0	78	42	36	-	0.468^b	23	55	-	1.000^b
N1	1	0	1	-	0.468^b	0	1	-	1.000^b
M stage
M0	77	42	35	-	0.216^b	23	54	-	1.000^b
M1	2	0	2	-	0.216^b	0	2	-	1.000^b
TNM stage
Ⅰ	58	33	25	1.220	0.269	19	39	1.404	0.236
Ⅱ/Ⅲ/Ⅳ	21	9	12	1.220	0.269	4	17	1.404	0.236
Tumor size (cm)
<6	48	25	23	0.057	0.811	18	30	4.168	0.041
≥6	31	17	14	0.057	0.811	5	26	4.168	0.041

Parameter (Cytoplasm)	Univariate analysis				Multivariate analysis
	HR	95%CI		P	HR	95%CI		P
	HR	Lower	Upper	P	HR	Lower	Upper	P
Expression of TRIM24 （High vs low）	3.353	1.552	7.243	0.002	3.523	1.520	8.168	0.003
Gender （Male vs female）	0.623	0.285	1.364	0.237	-	-	-	-
Age （≥60 years old vs <60 years old）	16.040	7.260	35.440	<0.001	0.975	0.221	4.304	0.974
Grade （Ⅲ/Ⅳ vs Ⅰ/Ⅱ）	25.040	5.859	107.000	<0.001	3.233	1.026	10.189	0.045
TNM stage （Ⅱ/Ⅲ/Ⅳ vs Ⅰ）	1.184	0.252	5.553	0.831	0.383	0.106	1.383	0.143
T stage （T3 vs T1/T2）	1.735	0.299	10.060	0.539	-	-	-	-
N stage （N1 vs N0）	22 . 929	33.870	15 .520 054	0.003	-	-	-	-
M stage （M1 vs M0）	2.723	0.167	44.380	0.482	-	-	-	-
Tumor size （≥6 cm vs <6 cm）	0.893	0.412	1.935	0.775	0.981	0.178	5.409	0.983

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Expression of TRIM24 protein in human clear cell renal cell carcinoma tissue and its clinical significance

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