PRDX6过表达对肝癌细胞增殖、侵袭和迁移的影响及其分子机制

doi:10.13481/j.1671-587X.20210304

Abstract

Abstract: Objective

To discuss the effect of over-expression of peroxiredoxin 6 （PRDX6） gene on the proliferation， invasion and migration of liver cancer cells， and to clarify its possible mechanism.

Methods

The HepG2 and Hep3B cells were established and divided into PRDX6 over-expression group（tansfected with over-expression vector PIRES-EGFP-PRDX6） and empty vector group（tansfected with control vector PIRES）.The expression levels of PRDX6 mRNA and protein in the liver cancer cells in two groups were detected by Real-time fluorescence quantitative（RT-qPCR） and Western blotting methods；the proliferation rates of the liver cancer cells in two groups were detected by MTT method in vitro；scratch assay and Transwell chamber assay were used to evaluate the wound healing rate and the invasion number of liver cancer cells in two groups； Western blotting method was used to detect the expression levels of PRDX6， matrix matalloproteinases-2（MMP-2）and matrix matalloproteinases-9（MMP-9）proteins in the liver cancer cells in two groups.

Results

The RT-qPCR and Western blotting results showed that compared with empty vector group， the expression levels of PRDX6 mRNA and protein in PRDX6 over-expression group were significantly increased （P<0.05）.The MTT results showed that compared with empty vector group， the proliferation activities of the liver cancer cells in PRDX6 over-expression group were significantly increased （P<0.05）. The scratch assay and Transwell chamber assay results showed that compared with empty vector group， the wound healing rates and the number of invasion liver cancer cells in PRDX6 over-expression group were significantly increased （P<0.05）.The Western blotting results showed that that compared with empty vector group， the expression levels of MMP-2 and MMP-9 proteins in the liver cancer cells in PRDX6 over-expression groups were increased（P<0.05）.

Conclusion

PRDX6 could significantly promote the proliferation， invasion， and migration of liver cancer cells， and its mechanism may be related to its regulatory effect on the expression levels of MMP- 2 and MMP- 9 proteins.

Key words: liver neoplasms, peroxiredoxin 6, cell proliferation, cell invasion, cell migration

CLC Number:

R735.7

Runhong MU, Xinzhu LIU, Rui LIN, Yupeng LI, Luyao WANG, Chunyu WANG, Xiao GUO. Effect of PRDX6 over-expression of proliferation, invasion and migration of liver cancer cells and its molecular mechanism[J].Journal of Jilin University(Medicine Edition), 2021, 47(3): 559-565.

Figures/Tables 6

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Tab.1

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Group		Proliferation activity
Group		(t/h) 4	24	48	72	96
Empty vector	HepG2 cells	0.183±0.023	0.224±0.029	0.371±0.017	0.515±0.033	0.857±0.048
Empty vector	Hep3B cells	0.133±0.018	0.227±0.023	0.326±0.025	0.437±0.033	0.763±0.042
PRDX6 over-expression	HepG2 cells	0.180±0.026	0.312±0.031^*	0.558±0.024^*	0.765±0.038^*	0.987±0.043^*
PRDX6 over-expression	Hep3B cells	0.139±0.021	0.312±0.027^*	0.488±0.032^*	0.655±0.035^*	0.887±0.039^*

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Effect of PRDX6 over-expression of proliferation, invasion and migration of liver cancer cells and its molecular mechanism

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