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Rapid Determination of Rifampicin and Isoniazide Tablets UsingNear Infrared Diffuse Reflectance Spectroscopy Combined with Partial Least Square
LU Jiahui, WU Ling, GUO Weiliang, TIAN Xiaole, CHEN Yufei, SHI Jian, TENG Lirong
J4. 2007, 45 (03):
481-485.
Near infrared diffuse reflectance spectroscopy combined with partial least square (NIRSPLS) was applied to establishing calibration models for determining the contents of rifampicin and isoniazide in rifampicin and isoniazide tablets.The regression coefficient (R) by means of the model for determining the content of rifampicinis 0.992 77, the root mean square error of crosscalibration (RMSECV) is 0.006 65, the R by means of the model for determining the content of isoniazide is 0.989 01, and the RMSECV is 0.00423.Using these models for predicting the contents of rifampicin and iso
niazide in validation set, the root mean square error of prediction (RMSEP) are
0.005 73 and 0.003 79.These results demonstrate that this method possesses high precision of prediction. The average recoveries are 99.376% and
98.243%in the recovery experiments, and the relative standard deviation (RSD) are 0.679 3% and 0.639 8% in reappearance experiments. With its advantages of facility, rapid, nondestruction, no pollution, online detection and good reappearance, this method must be popularized in situ measurement and in the online quality control for rifampicin and isoniazide tablets production.
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