吉林大学学报(医学版) ›› 2023, Vol. 49 ›› Issue (5): 1290-1295.doi: 10.13481/j.1671-587X.20230523

• 临床研究 • 上一篇    

2型糖尿病患者外周血淋巴细胞中CDKAL1基因及其剪接异构体的表达水平和临床意义

王嘉欣1,王珍琦2,张萱3()   

  1. 1.吉林大学第二医院内分泌科,吉林 长春 130041
    2.吉林大学公共卫生学院 国家卫健委放射生物学重点实验室,吉林 长春 130021
    3.吉林大学第二医院科研部,吉林 长春 130041
  • 收稿日期:2022-10-08 出版日期:2023-09-28 发布日期:2023-10-26
  • 通讯作者: 张萱 E-mail:zhangxuan@jlu.edu.cn
  • 作者简介:王嘉欣(1985-),女,吉林省长春市人,主治医师,医学博士,主要从事人类重大疾病发病机制方面的研究。
  • 基金资助:
    吉林省财政厅卫生专项项目(201817275775);吉林省卫健委卫生健康科技能力提升项目(2021JC039)

Expressions of CDKAL1 gene and its splice isomers in peripheral blood lymphocytes of patients with type 2 diabetes mellitus and their clinical significances

Jiaxin WANG1,Zhenqi WANG2,Xuan ZHANG3()   

  1. 1.Department of Endocrinology,Second Hospital,Jilin University,Changchun 130041,China
    2.Key Laboratory of Radiobiology,National Health Commission,School of Public Health,Jilin University,Changchun 130021,China
    3.Department of Science and Technology,Second Hospital,Jilin University,Changchun 130041,China
  • Received:2022-10-08 Online:2023-09-28 Published:2023-10-26
  • Contact: Xuan ZHANG E-mail:zhangxuan@jlu.edu.cn

摘要:

目的 探讨2型糖尿病(T2DM)患者外周血淋巴细胞中细胞周期蛋白依赖性激酶5调节亚基相关蛋白1类似物1(CDKAL1)基因及其剪接异构体的表达水平,并探讨其临床意义。 方法 收集65例糖尿病患者,其中T2DM患者20例(T2DM组)、糖尿病肾病(DN)患者23例(DN组)和糖尿病视网膜病变(DR)患者22例(DR组),并选择同期健康体检者28名(健康对照组)。采用实时荧光定量PCR(RT-qPCR)法检测各组研究对象外周血淋巴细胞中CDKAL1基因及其2种剪接异构体CDKAL1-X1和CDKAL1-X2表达水平,分析其表达与T2DM及其糖尿病微血管并发症的关系。 结果 与健康对照组比较,T2DM组患者外周血淋巴细胞中CDKAL1基因表达水平升高(Z=4.705,P<0.01),CDKAL1-X1表达水平升高(Z=2.698,P=0.007)。与健康对照组比较,DR组和DN组患者外周血淋巴细胞中CDKAL1基因和CDKAL1-X1表达水平升高(P<0.05); DR组患者外周血淋巴细胞中CDKAL1基因表达水平高于DN组(P<0.05)。 结论 T2DM患者外周血淋巴细胞中CDKAL1基因和CDKAL1-X1表达水平升高可能参与糖尿病及其并发症的发生发展。

关键词: 糖尿病,2型, 细胞周期蛋白依赖性激酶5调节亚基相关蛋白1类似物1, 基因表达, 糖尿病肾病, 糖尿病视网膜病变

Abstract:

Objective To discuss the expression levels of the cyclin-dependent kinase 5 regulatory subunit associated protein 1-like 1(CDKAL1) gene and its splice isoforms in peripheral blood lymphocytes of the patients with type 2 diabetes mellitus (T2DM), and to clarify their clinical significances. Methods A total of 65 diabetes mellitus patients were enrolled, including 20 T2DM patients (T2DM group), 23 diabetic nephropathy(DN) patients (DN group), 22 diabetic retinopathy(DR)patients (DR group), and 28 healthy examiners at the same term(healthy control group). Real-time fluorescence quantitative PCR (RT-qPCR) method was used to detect the expression levels of CDKAL1 gene and its two splice isoforms, CDKAL1-X1 and CDKAL1-X2, in peripheral blood lymphocytes of the subjects in various groups. The relationships between the clinical significances of their expressions and T2DM and its microvascular complications were analyzed. Results Compared with healthy control group, the expression levels of CDKAL1 gene (Z=4.705, P<0.01) and CDKAL1-X1 (Z=2.698, P=0.007) in peripheral blood lymphocytes of the patients in T2DM group were increased. Compared with healthy control group, the expression levels of CDKAL1 gene and CDKAL1-X1 in peripheral blood lymphocytes of the patients in DR and DN groups were increased(P<0.05); the expression level of CDKAL1 gene in peripheral blood lymphocytes of the patients in DR group was higher than that in DN group (P<0.05). Conclusion The high expressions of CDKAL1 gene and CDKAL1-X1 in peripheral blood lymphocytes of the T2DM patients may play an important role in the occurence and development of diabetic mellitus and its complications.

Key words: Type 2 diabetes mellitus, Cyclin-dependent kinase 5 regulatory subunit associated protein 1-like 1, Gene expression, Diabetic nephropathy, Diabetic retinopathy

中图分类号: 

  • R587.1