关键词: miR-205, YES1, 肺肿瘤, 细胞增殖

Abstract:

To detect the expression levels of the miR-205 in lung cancer tissue and A549 cells and its targeted gene YES1 using qRT-PCR and  dual fluorescence protein repoter assay system,and to explore the possible mechanism of miR-205 to inhibit the proliferation of lung cancer A549 cells.Methods The expression levels of miR-205 in 10 cases of lung cancer tissue and adjacent normal lung tissue were detected with qRT-PCR.The cell growth curve and colony formation assay were used to determine the proliferation rate of A549 cells after transfected by miR-205 mimics and control mimics.The sequences of YES1 3′UTR (untranslated region) and mutation target sites of YES1 3′UTR were inserted into the plasmid which expressed green fluorescence protein (pcDNA3/EGFP) respectively to construct the green fluorescence protein plasmids of YES1-3′UTR and mut-YES1- 3′UTR.There were six groups in the study: YES1-3′UTR,YES1-3′UTR and miR-205 mimics,YES1-3′UTR and control mimics,mut-YES1-3′UTR,mut-YES1-3′UTR and miR-205 mimics,mut-YES1- 3′UTR and control mimics;after the plasmids expressed red fluorescent protein (pDsRed2 -N1 ) were cotransfected into A549 cells,the extracted protein was detected with  fluorescence spectrophotometer.Results Compared with adjacent normal lung tissue,the expression levels of miR-205 in lung cancer tissue and A549 cells were decreased (P<0.05); the proliferation rate of A549 cells in miR-205 mimics group was lower than that in control mimics group (P<0.05).The fluorescence protein expression level in YES1-3′UTR and miR-205 mimics co-transfected group was lower than that in  YES1-3′UTR and control mimics co-transfected group,the difference was statistically significant (P<0.01). The number of cell colony formation of A549 cells in highly expressed YES1  group  was higher than that in cell control group (P<0.05).Conclusion MiR-205 may inhibit the proliferation of A549 cells  through regulating of the expression of YES1 directly.miR-205 and YES1 are potential therapeutic targets for the biological trea
tment of tumor.

Key words: miR-205, v-yes-1 yamaguchi sarcoma viral oncogene homolog 1, lung neoplasms, cell proliferation