吉林大学学报(医学版) ›› 2019, Vol. 45 ›› Issue (03): 621-626.doi: 10.13481/j.1671-587x.20190325

• 临床研究 • 上一篇    

人结直肠癌和肿瘤引流淋巴结组织中Foxp3+调节性T细胞和髓样树突状细胞的表达及其意义

王丹1, 宋紫绮1, 李怡飞2, 历春1, 董志恒1, 董营1, 盖晓东1   

  1. 1. 北华大学医学院病理教研室, 吉林 吉林 132013;
    2. 吉林省吉林市中心医院骨关节科, 吉林 吉林 132011
  • 收稿日期:2018-08-21 发布日期:2019-06-05
  • 通讯作者: 盖晓东,教授,医学博士,硕士研究生导师(Tel:0432-64608104,E-mail:bhdxgaixiaodong@126.com) E-mail:bhdxgaixiaodong@126.com
  • 作者简介:王丹(1975-),女,吉林省吉林市人,副教授,医学博士,主要从事肿瘤分子病理学方面的研究。
  • 基金资助:
    吉林省科技厅科研基金资助课题(20170414030GH)

Expressions of Foxp3+ regulatory T cells and myeloid dentritic cells in human colorectal cancer and tumor draining lymph node tissues and their significances

WANG Dan1, SONG Ziqi1, LI Yifei2, LI Chun1, DONG Zhiheng1, DONG Ying1, GAI Xiaodong1   

  1. 1. Department of Pathology, School of Medical Sciences, Beihua University, Jilin University, Jilin 132013, China;
    2. Department of Orthopaedics, Jilin Central Hospital, Jilin Province, Jilin 132011, China
  • Received:2018-08-21 Published:2019-06-05

摘要: 目的:探讨Foxp3+调节性T细胞(Tregs)和CD11c+髓样树突状细胞(mDCs)在结直肠癌(CRC)组织中的表达及在肿瘤引流淋巴结(TDLN)组织中的浸润,并探讨其临床意义。方法:收集CRC患者手术切除的标本52例,采用免疫组织化学方法检测CRC患者标本及TDLN组织样本中Foxp3+ Tregs及CD11c+mDCs的表达,并探讨其表达频数与CRC患者临床病理特征的关系。结果:CRC患者癌组织中Foxp3+Tregs的阳性表达频数高于癌旁正常组织(P<0.01);淋巴结转移阳性者癌组织中Foxp3+Tregs阳性表达频数高于淋巴结转移阴性者(P<0.01);TNM分期Ⅲ+Ⅳ期组患者癌组织中Foxp3+Tregs阳性表达频数高于TNM分期Ⅰ+Ⅱ期组患者(P<0.01);肿瘤引流阳性淋巴结(mTDLN)组织中Foxp3+ Tregs阳性表达频数明显高于肿瘤引流阴性淋巴结(mfTDLN)组织(P<0.01)。CRC患者癌组织中CD11c+ mDCs的阳性表达频数高于癌旁正常组织(P<0.01);淋巴结转移阳性者癌组织中CD11c+ mDCs阳性表达频数低于淋巴结转移阴性者(P<0.01);TNM分期Ⅲ+Ⅳ期组患者癌组织中CD11c+ mDCs阳性表达频数低于TNM分期Ⅰ+Ⅱ期组患者(P<0.01);不同浸润深度癌组织中CD11c+ mDCs阳性表达频数比较差异有统计学意义(P<0.05);mTDLN组织中CD11c+ mDCs阳性表达频数明显低于mfTDLN组织(P<0.01)。CRC和TDLN组织中Foxp3+ Tregs表达与CD11c+mDCs的表达无相关性(P>0.05)。结论:CRC的发生发展与癌组织局部微环境中Foxp3+ Tregs及CD11c+ mDCs的表达频数有关。Foxp3+ Tregs和CD11c+mDCs在抑制肿瘤微环境的细胞免疫中发挥作用。

关键词: 结直肠肿瘤, 肿瘤引流淋巴结, 肿瘤转移, 调节性T细胞, 髓样树突状细胞

Abstract: Objective:To explore the expressions of Foxp3+ Tregs and CD11c+ mDCs in the colorectal cancer (CRC) tissue and their infiltrations in the tumor draining lymph node (TDLN) tissue,and to explore the clinical significances. Methods:Fifty-two samples of surgical resection of the CRC patients were collected. The expressions of Foxp3+ Tregs and CD11c+ mDCs in the specimens of the CRC patients and TDLN tissue were detected by immunohistochemistry, and the relationship between the expression frequencies of Foxp3+ Tregs and CD11c+ mDCs and the clinicopathological characteristics of the patients were discussed. Results:The positive expression frequency of Foxp3+ Tregs in cancer tissue of the CRC patients was higher than that in adjacent normal mucosa tissue(P<0.01); The expression frequency of Foxp3+ Tregs in cancer tissue in the patients with positive lymph node metastasis was higher than that in the patients with negative lymph node metastasis(P<0.01); the expression frequency of Foxp3+ Tregs in the patients in TNM stage Ⅲ+Ⅳ was higher than that in TNM stage Ⅰ+Ⅱ(P<0.01); the positive expression frequency of Foxp3+ Tregs in metastatic TDLN (mTDLN) tissue was higher than that in metastatic free TDLN (mfTDLN) tissue(P<0.01). The positive expression frequency of CD11c+ mDCs in cancer tissue of the CRC patients was higher than that in adjacent normal mucosa tissue(P<0.01); the positive expression frequency of CD11c+ mDCs in the patients with positive lymph node metastasis was lower than that in the patients with negative lymph node metastasis(P<0.01);the positive expression frequency of CD11c+ mDCs in the patients in TNM stage Ⅲ+Ⅳ was lower than that in the patients in TNM stage Ⅰ+Ⅱ(P<0.01); the positive expression frequencies of CD11c+ mDCs in cancer tissue with different depths invasion were significantly different (P<0.05);the positive expression frequency of CD11c+ mDCs in mTDLN tissue was lower than that in mfTDLN tissue (P<0.01).There were no significant correlations between the expressions of Foxp3+ Tregs and CD11c+ mDCs in the CRC and TDLN tissues(P>0.05). Conclusion:The occurrence and development of CRC are related to the changes of Foxp3+ Tregs and CD11c+ mDCs expression frequencies in the local microenvironment of cancer tissue.Foxp3+ Tregs and CD11c+ mDCs play a role in inhibiting the cellular immunity in tumor microenvironment.

Key words: colorectal neoplasms, tumor draining lymph nodes, tumor metastasis, regulatory T cells, myeloid dentritic cells

中图分类号: 

  • R735.35