关键词: 骨髓间充质干细胞；肾缺血-再灌注；修复

Abstract:

Abstract:Objective To study the capability of bone marrow mesenchymal stem cells (BMSCs) in promoting the recovery of renal ischemia-reperfusion injury and provide a new method for the treatment of renal diseases. Methods BMSCs were cultivated and amplified in vitro from bone marrow cell suspension of healthy 3-week-old C57BL/6J mice.8-week-old C57BL/6J mice were randomly divided into sham operation group (n=16),parallel control group (n=16) and cell transplantation group (n=16).The renal ischemia-reperfusion injury model was established.BMSCs were transplanted into the mice in cell transplantation group via the tail vein and saline was injected into the mice in parallel control group.Renal function,such as the level of serum urea nitrogen (BUN) and creatinine (Cr),the indexes of renal oxidative stress,such as the levels of hydroxyl radical and superoxide anion,morphologic changes and renal pathological changes were detected. Results ①BMSCs not only had a high purification,homogeneity and stability,but also had unlimited proliferation.②The mice in parallel control group appeared the poor state.The indexes of renal function and oxidative stress were obviously increased.And there were collapse of renal glomerulus,disappearance of renal tubule and infiltration of inflammatory cells in renal tissue.Transplanted mice appeared the good state.The indexes of renal function and indexes of oxidative stress had been significantly improved (P<0.05).The renal pathological changes were not obvious. Conclusion BMSCs are easily isolated and cultivated in vitro.BMSCs can improve renal ischemia-reperfusion injury via vein transplantation by inhibiting the production and attacking of oxygen free radicals.

Key words: bone marrow mesenchymal stem cells;renal ischemia- reperfusion;repair