关键词: 山楂叶总黄酮；心肌缺血；缺血再灌注；脂质过氧化；一氧化氮

Abstract:

Objective To investigate the protective effects of Flavone mixture of crataegus leave (FMCL) on the rat myocardial ischemia reperfusion models and their mechanisms.Methods Fifty-six Wistar rats were randomly divided into control group,sham group,ischemia reperfusion model group,Xue-Sai-Tong(20 mg/kg) group and FMCL (30.0，15.0，7.5  mg/kg) groups(n=8).The myocardial ischemia reperfusion model was induced by 30 min left anterior descending coronary occulusion and 120 min reperfusion in openchest anesthetized rats to study the effects of FMCL on elegated ST-T of electrocardiogram ( ECG),the size of myocardial infarction,the morphological changes of cardiocytes and the contents of SOD,MDA,CK,AST,LDH and NO in serum were detected. Results Compared with model group,elegated ST-T of  ECG in FMCL（30.0,15.0 mg/kg）and Xue-Sai-Tong groups were decreased（P<0.01）,the sizes of myocardial infarction in FMCL（30.0,15.0,7.5 mg/kg）　and Xue-Sai-Tong groups were shrinked（P<0.05 or P<0.01）,FMCL could ameliorate the pathological lesion. Compared with model group,the contents of AST,CK,LDH and MDA in FMCL（30.0,15.0 mg/kg）and Xue-Sai-Tong groups were decreased（P<0.05 or P<0.01）,and the contents of SOD and NO were increased（P<0.01）.Conclusion FMCL  has the protective effects on myocardial ischemia reperfusion injury in rats,which may be related to inhibition of lipid oxidation.

Key words: Flavone mixture of crataegus leave；myocardial ischemia；ischemia reperfusion；lipid peroxidation；nitric oxide