D-半乳糖对仔鼠脂质过氧化、听力和耳蜗毛细胞的损伤及其机制

J4 ›› 2012, Vol. 38 ›› Issue (3): 419-422.

D-半乳糖对仔鼠脂质过氧化、听力和耳蜗毛细胞的损伤及其机制

郝帅¹|于飞²|杨博¹|闫艾慧¹|姜学钧¹

（1. 中国医科大学第一临床医院耳鼻咽喉科|辽宁沈阳 110001;2. 中国医科大学公共卫生学院营养与食品卫生学教研室|辽宁沈阳 110001）

收稿日期:2011-06-24 出版日期:2012-05-28 发布日期:2012-05-28
通讯作者: 姜学钧 E-mail:（Tel：024-83283566,E-mail：xjjiang_cmu@yahoo.cn）
作者简介:郝帅（1980-）|女|辽宁省大连市人|主治医师|医学博士|主要从事耳鼻发育相关疾病的研究。
基金资助:
国家自然科学基金资助课题（30901209）

Injury of lipid peroxidation, hearing and |cochlear hair cells in duced by D-galactose |in newborn rats　and its mechanism

HAO Shuai¹,YU Fei²,YANG Bo¹,YAN Ai-hui¹,JIANG Xue-jun¹

(1. Department of Otolaryngology,First Affiliated Hospital,China Medical University,Shenyang 110001,China;2. Department of Nutrition and Food Hygiene,School of Public Health,China Medical University,Shenyang 110001,China)

Received:2011-06-24 Online:2012-05-28 Published:2012-05-28

摘要/Abstract

摘要：

目的：观察孕期和哺乳期D-半乳糖暴露对仔鼠脂质过氧化、听力功能和耳蜗毛细胞形态结构的影响，阐明其与仔鼠听力障碍-机体脂质过氧化增强之间的关系。方法： 12只成功受孕大鼠随机分为D-半乳糖组和对照组，每组6只。自妊娠6 d起，对照组孕鼠给予普通饲料，D-半乳糖组孕鼠给予普通饲料配30% D-半乳糖的混合饲料。仔鼠出生后21 d，检测仔鼠血清D-半乳糖、全血超氧化物歧化酶（SOD）、还原型谷胱甘肽（GSH）、谷胱甘肽过氧化物酶（GSH-Px）和血浆丙二醛（MDA）含量变化，检测畸变产物耳声发射(DPOAE)情况，应用硝酸银染色全耳蜗铺片观察仔鼠毛细胞形态学变化。结果：孕期和哺乳期D-半乳糖暴露致仔鼠全血SOD、GSH-Px和GSH水平明显低于对照组（P<0.05），D-半乳糖组仔鼠血浆MDA水平高于对照组（P<0.05）；D-半乳糖组仔鼠在各频率上DPOAE幅值均低于对照组（P<0.05）；D-半乳糖组仔鼠耳蜗外毛细胞结构受损，且数目缺失。结论：Ｄ-半乳糖可诱导仔鼠氧化损伤和听觉发育损伤。

关键词: 脂质过氧化；耳毒性；D-半乳糖

Abstract:

To observe the influence of pregnant and lactational D-galactose exposure in lipid peroxidation,hearing function and morphology and structures of cochlear hair cells in newborn rats,and to clarify the relation with cochlear hair cell injury,hearing loss and increased lipid peroxidation.Methods 12 pregnant rats were randomly devided into control group (n=6) and D-galactose group (n= 6) from gestational day 6,the rats in control group were fed on standard rodent diet,and the rats in D-galactose group were fed on standard rodent diet supplemented with 30% D-galactose.On postnatal day 21,the blood levels of galactose,superoxide dismutase(SOD),glutathion(GSH),glutathione peroxidase(GSH-Px) and malondialdethyde(MDA) in newborn rats were measured. Then the distortion product otoacoustic emission (DPOAE) was examined.The temporal bones were then removed and the morphological changes were detected by silver nitrate staining.Results The levels of blood SOD,GSH-Px and GSH were lower in newborn rats after using D-galactose during pregnancy and lactation compared with control group (P<0.05),and the plasma MDA level was higher than that in control group (P<0.05).In addition,the DPOAE threshold shifts at different frequencies were lower after using D-galactose during pregnancy and lactation compared with control group (P<0.05).The structures of cochlea hair cells were impaired and the number of hair cells was decreased in D-galactose group.Conclusion D-galactose exposure may cause oxidative and cochlear function damage in newborn rats.

Key words: lipid peroxidation;ototoxicity;D-galactose

中图分类号:

郝帅|于飞|杨博|闫艾慧|姜学钧. D-半乳糖对仔鼠脂质过氧化、听力和耳蜗毛细胞的损伤及其机制[J]. J4, 2012, 38(3): 419-422.

HAO Shuai,YU Fei,YANG Bo,YAN Ai-hui,JIANG Xue-jun. Injury of lipid peroxidation, hearing and |cochlear hair cells in duced by D-galactose |in newborn rats　and its mechanism[J]. J4, 2012, 38(3): 419-422.

[1]	周颖,高孟飞,朱慧勇. 间充质干细胞干性维持信号通路的研究进展[J]. 吉林大学学报(医学版), 2013, 39(3): 638-642.
[2]	李红，陈永浩，龚红君. 右美托咪定对肾癌根治术患者围术期炎性细胞因子和肾功能的影响[J]. 吉林大学学报(医学版), 2013, 39(3): 588-591.
[3]	李海波，刘行，孙美花,梁建民. 孕期至幼年期低水平铅暴露仔鼠海马锌离子平衡的变化及其发育期海马神经元的损伤[J]. 吉林大学学报(医学版), 2013, 39(3): 521-528.
[4]	谢波，沈梅，叶文鑫，舒彦，梁臻，张心男. 依帕司他联合行为疗法治疗女性糖尿病膀胱的临床疗效评价[J]. 吉林大学学报(医学版), 2013, 39(3): 597-600.
[5]	李辉,赵丽晶,杜宇,杨铁铮，李国峰,陈亮,支晨阳,李娜,周建华. 结肠安栓联合舒血宁对溃疡性结肠炎大鼠的治疗作用及其机制[J]. 吉林大学学报(医学版), 2013, 39(3): 488-493.
[6]	张洪长，王恩鹏，陈新，唐燕，王洪鑫，王炫策，许新. 人参皂苷Re对UVC辐射损伤细胞的保护作用[J]. 吉林大学学报(医学版), 2013, 39(3): 507-511.
[7]	武国东，王素侠，及志勇，张舒岩. 急性心力衰竭大鼠B型钠尿肽水平与肝功能的关系[J]. 吉林大学学报(医学版), 2013, 39(3): 517-520.
[8]	陈宣男，全首祯. NDM1基因常规PCR与荧光定量PCR检测方法的建立及其检测结果比较[J]. 吉林大学学报(医学版), 2013, 39(3): 625-629.
[9]	周超熙,于滨,赵斌,马志强,赵文河,于跃明. 直肠癌环周切缘病理学检测判断中低位直肠癌患者预后的临床价值[J]. 吉林大学学报(医学版), 2013, 39(3): 601-604.
[10]	周广宇，韩雪梅，金玲. 神经精神狼疮患者影像学表现与自身抗体水平和预后的关系[J]. 吉林大学学报(医学版), 2013, 39(3): 609-614.
[11]	周建英，田勇韩青,贺庆,程凯亮,李幼琼. 影像学三维重建技术在寰椎立体模型建立及性别鉴定中的应用[J]. 吉林大学学报(医学版), 2013, 39(3): 605-608.
[12]	沈煜，沈惠良，方秀统. 老年股骨颈骨折患者关节置换术后死亡危险因素分析[J]. 吉林大学学报(医学版), 2013, 39(3): 574-577.
[13]	王宗谦，卢新星. 终末期肾病患者抑郁程度与生存质量各个维度之间的关系[J]. 吉林大学学报(医学版), 2013, 39(3): 615-619.
[14]	李树春，王槐. 影响美托洛尔治疗效果的药物基因组学研究进展[J]. 吉林大学学报(医学版), 2013, 39(2): 423-426.
[15]	胡源，贾卉，姜瑞，李京，刘涛涛. 不同验光方法对中年患者屈光状态的检测及其临床意义[J]. 吉林大学学报(医学版), 2013, 39(2): 363-366.